Escherichia coli K-12 substr. MG1655 Protein: AtoS sensory histidine kinase

Gene: atoS Accession Numbers: EG11667 (EcoCyc), b2219, ECK2212

Regulation Summary Diagram: ?

Regulation summary diagram for atoS

Subunit composition of AtoS sensory histidine kinase = [AtoS]2
         AtoS monomer = AtoS

Alternative forms of AtoS sensory histidine kinase: AtoS sensory histidine kinase - phosphorylated (summary available)

AtoS is the sensor histidine kinase of the AtoS/AtoC two-component signal transduction pathway which is best characterised by its induction of the ato operon for metabolism of short chain fatty acids in response to the presence of acetoacetate. AtoS is a homo-dimeric transmembrane protein consisting of an amino-terminal periplasmic sensing domain coupled to a carboxy-terminal cytoplasmic kinase domain [Lioliou05, Filippou08]. AtoS autophosphorylates on a conserved histidine residue by trans-phosphorylation between the monomers of the homodimer [Filippou08]. AtoS tranfers a phosphoryl group to the cytoplasmic response regulator AtoC which controls transcriptional expression of the operon (atoDAEB) involved in short-chain fatty acid catabolism [Jenkins87, Lioliou04, Lioliou05].

Locations: inner membrane

Map Position: [2,318,065 -> 2,319,891] (49.96 centisomes, 180°)
Length: 1827 bp / 608 aa

Molecular Weight of Polypeptide: 67.79 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007341 , CGSC:37061 , EchoBASE:EB1618 , EcoGene:EG11667 , EcoliWiki:b2219 , ModBase:Q06067 , OU-Microarray:b2219 , PortEco:atoS , PR:PRO_000022185 , Pride:Q06067 , Protein Model Portal:Q06067 , RegulonDB:EG11667 , SMR:Q06067 , String:511145.b2219 , UniProt:Q06067

Relationship Links: InterPro:IN-FAMILY:IPR000014 , InterPro:IN-FAMILY:IPR000700 , InterPro:IN-FAMILY:IPR003594 , InterPro:IN-FAMILY:IPR003660 , InterPro:IN-FAMILY:IPR003661 , InterPro:IN-FAMILY:IPR004358 , InterPro:IN-FAMILY:IPR005467 , InterPro:IN-FAMILY:IPR009082 , InterPro:IN-FAMILY:IPR013767 , Pfam:IN-FAMILY:PF00512 , Pfam:IN-FAMILY:PF00672 , Pfam:IN-FAMILY:PF00989 , Pfam:IN-FAMILY:PF02518 , Prints:IN-FAMILY:PR00344 , Prosite:IN-FAMILY:PS50109 , Prosite:IN-FAMILY:PS50112 , Prosite:IN-FAMILY:PS50113 , Prosite:IN-FAMILY:PS50885 , Smart:IN-FAMILY:SM00091 , Smart:IN-FAMILY:SM00304 , Smart:IN-FAMILY:SM00387 , Smart:IN-FAMILY:SM00388

In Paralogous Gene Group: 122 (29 members)

Reactions known to consume the compound:

AtoSC Two-Component Signal Transduction System :
AtoS monomer + ATP → AtoS-P + ADP

Reactions known to produce the compound:

AtoSC Two-Component Signal Transduction System :
AtoS-P + AtoC → AtoS monomer + AtoC-P

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Filippou08, Lioliou05]
GO:0023014 - signal transduction by protein phosphorylation Inferred from experiment Inferred by computational analysis [GOA01a, Filippou08]
GO:0046777 - protein autophosphorylation Inferred from experiment [Filippou08]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [GOA01a]
GO:0007165 - signal transduction Inferred by computational analysis [GOA01a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0018106 - peptidyl-histidine phosphorylation Inferred by computational analysis [Gaudet10]
Molecular Function: GO:0000155 - phosphorelay sensor kinase activity Inferred from experiment Inferred by computational analysis [GOA01a, Filippou08]
GO:0005524 - ATP binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Filippou08]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0004673 - protein histidine kinase activity Inferred by computational analysis [GOA01]
GO:0004871 - signal transducer activity Inferred by computational analysis [GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005622 - intracellular Inferred by computational analysis Inferred from experiment [Filippou08, Lioliou05, UniProtGOA11a, GOA01a]
GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Larson96, Lioliou05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a, Filippou08]

MultiFun Terms: cell structure membrane
information transfer protein related posttranslational modification
metabolism carbon utilization fatty acids
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for atoS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of AtoS monomer with features indicated

Feature Class Location Citations Comment State
Transmembrane-Region 16 -> 36
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 190 -> 210
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 212 -> 259
UniProt: HAMP;
Conserved-Region 260 -> 325
UniProt: PAS;
Conserved-Region 326 -> 382
UniProt: PAC;
Conserved-Region 395 -> 602
UniProt: Histidine kinase;
Phosphorylation-Modification 398
UniProt: Phosphohistidine; by autocatalysis; Non-Experimental Qualifier: by similarity.
Transmembrane-Region 584 -> 604
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b2219 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11667; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Filippou08: Filippou PS, Kasemian LD, Panagiotidis CA, Kyriakidis DA (2008). "Functional characterization of the histidine kinase of the E. coli two-component signal transduction system AtoS-AtoC." Biochim Biophys Acta 1780(9);1023-31. PMID: 18534200

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jenkins87: Jenkins LS, Nunn WD (1987). "Regulation of the ato operon by the atoC gene in Escherichia coli." J Bacteriol 1987;169(5);2096-102. PMID: 2883171

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Larson96: Larson E (1996). "The soul of an HMO." Time 147(4);44-52. PMID: 10153782

Lioliou04: Lioliou EE, Kyriakidis DA (2004). "The role of bacterial antizyme: From an inhibitory protein to AtoC transcriptional regulator." Microb Cell Fact 3(1);8. PMID: 15200682

Lioliou05: Lioliou EE, Mimitou EP, Grigoroudis AI, Panagiotidis CH, Panagiotidis CA, Kyriakidis DA (2005). "Phosphorylation activity of the response regulator of the two-component signal transduction system AtoS-AtoC in E. coli." Biochim Biophys Acta 1725(3);257-68. PMID: 16153782

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Filippou07: Filippou PS, Lioliou EE, Panagiotidis CA, Athanassopoulos CM, Garnelis T, Papaioannou D, Kyriakidis DA (2007). "Effect of polyamines and synthetic polyamine-analogues on the expression of antizyme (AtoC) and its regulatory genes." BMC Biochem 8;1. PMID: 17224065

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sat Oct 3, 2015, biocyc13.