Escherichia coli K-12 substr. MG1655 Polypeptide: protein involved in cytokinesis

Gene: yceG Accession Numbers: EG11494 (EcoCyc), b1097, ECK1083

Regulation Summary Diagram: ?

Regulation summary diagram for yceG

A yceG deletion mutant shows impaired membrane invagination and cytokinesis and reduced sensitivity to ampicillin [Babu11b].

yceG may be part of a 5-gene operon consisting of the pabC, yceG, tmk, holB, and ycfH genes. A strain containing a miniTn10 insertion in yceG is described [Chaperon05].

Map Position: [1,153,335 -> 1,154,357] (24.86 centisomes, 89°)
Length: 1023 bp / 340 aa

Molecular Weight of Polypeptide: 38.247 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003712 , EchoBASE:EB1457 , EcoGene:EG11494 , EcoliWiki:b1097 , OU-Microarray:b1097 , PortEco:yceG , Pride:P28306 , Protein Model Portal:P28306 , RefSeq:NP_415615 , RegulonDB:EG11494 , SMR:P28306 , String:511145.b1097 , UniProt:P28306

Relationship Links: InterPro:IN-FAMILY:IPR003770 , InterPro:IN-FAMILY:IPR013087 , PDB:Structure:2R1F , Pfam:IN-FAMILY:PF02618

GO Terms:

Biological Process: GO:0000910 - cytokinesis Inferred from experiment [Babu11b]
Molecular Function: GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA01a]

MultiFun Terms: cell processes cell division

Essentiality data for yceG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 11-Oct-2012 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b1097 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11494; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Babu11b: Babu M, Diaz-Mejia JJ, Vlasblom J, Gagarinova A, Phanse S, Graham C, Yousif F, Ding H, Xiong X, Nazarians-Armavil A, Alamgir M, Ali M, Pogoutse O, Pe'er A, Arnold R, Michaut M, Parkinson J, Golshani A, Whitfield C, Wodak SJ, Moreno-Hagelsieb G, Greenblatt JF, Emili A (2011). "Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways." PLoS Genet 7(11);e1002377. PMID: 22125496

Chaperon05: Chaperon DN (2005). "A method for the construction of in frame substitutions in operons: Deletion of the essential Escherichia coli holB gene coding for a subunit of the DNA polymerase III holoenzyme." J Microbiol Methods NIL. PMID: 16083981

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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