Escherichia coli K-12 substr. MG1655 Transporter: RhtC threonine Rht Transporter

Gene: rhtC Accession Numbers: EG11468 (EcoCyc), b3823, ECK3817

Synonyms: yigJ

Regulation Summary Diagram: ?

Regulation summary diagram for rhtC

RhtC is a probable threonine efflux transporter. Expression of the rhtC gene conferred resistance to threonine [Zakataeva99]. RhtC is related to the RhtB homoserine efflux transporter and is a member of the Rht family of amino acid efflux proteins [Aleshin99]. It seems likely that RhtC functions as a threonine/proton antiport system.

E. coli rhtC has been heterologously expressed in Corynebacterium glutamicum resulting in maximal L-threonine excretion rates of 11.2 nmol min-1mg-1 as compared to 2.3 nmol nmol min-1 mg-1 obtained without rhtC expression which has been suggested to be of industrial significance [Diesveld08].

Locations: inner membrane

Map Position: [4,005,780 -> 4,006,400] (86.34 centisomes, 311°)
Length: 621 bp / 206 aa

Molecular Weight of Polypeptide: 22.474 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012487 , EchoBASE:EB1436 , EcoGene:EG11468 , EcoliWiki:b3823 , OU-Microarray:b3823 , PortEco:rhtC , PR:PRO_000023749 , Protein Model Portal:P0AG38 , RefSeq:YP_026264 , RegulonDB:EG11468 , String:511145.b3823 , UniProt:P0AG38

Relationship Links: InterPro:IN-FAMILY:IPR001123 , InterPro:IN-FAMILY:IPR004778 , Panther:IN-FAMILY:PTHR30086 , Pfam:IN-FAMILY:PF01810

In Paralogous Gene Group: 347 (4 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids threonine
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for rhtC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: Threonine Rht transporter (RhtC threonine Rht Transporter)

Transport reaction diagram for Threonine Rht transporter

Sequence Features

Protein sequence of RhtC threonine Rht Transporter with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 1 -> 21
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 44 -> 64
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 67 -> 87
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 150 -> 173
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Suzanne Paley on Thu Oct 21, 2004:
Position updated based on U00096.2 release of genome
10/20/97 Gene b3823 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11468.


Aleshin99: Aleshin VV, Zakataeva NP, Livshits VA (1999). "A new family of amino-acid-efflux proteins." Trends Biochem Sci 1999;24(4);133-5. PMID: 10322417

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Diesveld08: Diesveld R, Tietze N, Furst O, Reth A, Bathe B, Sahm H, Eggeling L (2008). "Activity of Exporters of Escherichia coli in Corynebacterium glutamicum, and Their Use to Increase L-Threonine Production." J Mol Microbiol Biotechnol NIL. PMID: 18594129

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zakataeva99: Zakataeva NP, Aleshin VV, Tokmakova IL, Troshin PV, Livshits VA (1999). "The novel transmembrane Escherichia coli proteins involved in the amino acid efflux." FEBS Lett 1999;452(3);228-32. PMID: 10386596

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Oct 4, 2015, biocyc13.