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Escherichia coli K-12 substr. MG1655 Polypeptide: flagellar biosynthesis protein FliT



Gene: fliT Accession Numbers: EG11389 (EcoCyc), b1926, ECK1925

Regulation Summary Diagram: ?

Summary:
FliT is involved in flagellar motility [Kawagishi92].

FliT function was primarily investigated in Salmonella typhimurium [Kutsukake94, Fraser99, Bennett01, Yamamoto06]. FliT, along with FliS and FlgN, may function as cytoplasmic, substrate-specific chaperones of the flagellar export system. They share several similarities with other type III cytoplasmic chaperone family members [Kutsukake94]. Affinity blot analysis showed that FliT binds in vitro to flagellar cap subunits (FliD) [Fraser99], suggesting that FliT may act as a chaperone which aids in the export and prevents premature aggregation of the FliD flagellar cap component [Bennett01]. Gel filtration chromatography indicate that FliT exists as a homodimer in the cytosol and that these homodimers bind to monomers of FliD to form heterotrimeric chaperone-substrate complexes [Bennett01].

Gene Citations: [Yokoseki95]

Locations: cytosol

Map Position: [2,003,737 -> 2,004,102] (43.19 centisomes)
Length: 366 bp / 121 aa

Molecular Weight of Polypeptide: 13.829 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006411 , CGSC:30739 , EchoBASE:EB1362 , EcoGene:EG11389 , EcoliWiki:b1926 , OU-Microarray:b1926 , PortEco:fliT , PR:PRO_000022669 , Protein Model Portal:P0ABY2 , RefSeq:NP_416436 , RegulonDB:EG11389 , SMR:P0ABY2 , String:511145.b1926 , UniProt:P0ABY2

Relationship Links: InterPro:IN-FAMILY:IPR008622 , Pfam:IN-FAMILY:PF05400

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0071973 - bacterial-type flagellum-dependent cell motility Inferred from experiment [Kawagishi92]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006457 - protein folding Inferred by computational analysis [GOA06]
GO:0044781 - bacterial-type flagellum organization Inferred by computational analysis [UniProtGOA11a]
GO:1902209 - negative regulation of bacterial-type flagellum assembly Inferred by computational analysis [GOA06]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14, Rajagopala09, Fraser99]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, GOA06]
GO:0005829 - cytosol Inferred by computational analysis [UniProtGOA11, DiazMejia09]

MultiFun Terms: cell processes motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
cell structure flagella
information transfer protein related chaperoning, repair (refolding)
metabolism biosynthesis of macromolecules (cellular constituents) flagellum

Essentiality data for fliT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 07-Jul-2014 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Protein-Segment 1 -> 50
[UniProt10]
UniProt: Required for homodimerization; Sequence Annotation Type: region of interest; Non-Experimental Qualifier: by similarity;
Protein-Segment 60 -> 98
[UniProt10]
UniProt: FliD binding; Sequence Annotation Type: region of interest; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b1926 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11389; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bennett01: Bennett JC, Thomas J, Fraser GM, Hughes C (2001). "Substrate complexes and domain organization of the Salmonella flagellar export chaperones FlgN and FliT." Mol Microbiol 39(3);781-91. PMID: 11169117

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Fraser99: Fraser GM, Bennett JC, Hughes C (1999). "Substrate-specific binding of hook-associated proteins by FlgN and FliT, putative chaperones for flagellum assembly." Mol Microbiol 32(3);569-80. PMID: 10320579

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kawagishi92: Kawagishi I, Muller V, Williams AW, Irikura VM, Macnab RM (1992). "Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes." J Gen Microbiol 1992;138 ( Pt 6);1051-65. PMID: 1527488

Kutsukake94: Kutsukake K (1994). "Excretion of the anti-sigma factor through a flagellar substructure couples flagellar gene expression with flagellar assembly in Salmonella typhimurium." Mol Gen Genet 243(6);605-12. PMID: 8028576

Rajagopala09: Rajagopala SV, Hughes KT, Uetz P (2009). "Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins." Proteomics 9(23);5296-302. PMID: 19834901

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yamamoto06: Yamamoto S, Kutsukake K (2006). "FliT acts as an anti-FlhD2C2 factor in the transcriptional control of the flagellar regulon in Salmonella enterica serovar typhimurium." J Bacteriol 188(18);6703-8. PMID: 16952964

Yokoseki95: Yokoseki T, Kutsukake K, Ohnishi K, Iino T (1995). "Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium." Microbiology 1995;141 ( Pt 7);1715-22. PMID: 7551038

Other References Related to Gene Regulation

Chilcott00: Chilcott GS, Hughes KT (2000). "Coupling of flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli." Microbiol Mol Biol Rev 64(4);694-708. PMID: 11104815

Helmann87: Helmann JD, Chamberlin MJ (1987). "DNA sequence analysis suggests that expression of flagellar and chemotaxis genes in Escherichia coli and Salmonella typhimurium is controlled by an alternative sigma factor." Proc Natl Acad Sci U S A 84(18);6422-4. PMID: 3306678

Ide99: Ide N, Ikebe T, Kutsukake K (1999). "Reevaluation of the promoter structure of the class 3 flagellar operons of Escherichia coli and Salmonella." Genes Genet Syst 74(3);113-6. PMID: 10586520

Ko00a: Ko M, Park C (2000). "Two novel flagellar components and H-NS are involved in the motor function of Escherichia coli." J Mol Biol 303(3);371-82. PMID: 11031114

Kundu97: Kundu TK, Kusano S, Ishihama A (1997). "Promoter selectivity of Escherichia coli RNA polymerase sigmaF holoenzyme involved in transcription of flagellar and chemotaxis genes." J Bacteriol 179(13);4264-9. PMID: 9209042

Park01: Park K, Choi S, Ko M, Park C (2001). "Novel sigmaF-dependent genes of Escherichia coli found using a specified promoter consensus." FEMS Microbiol Lett 2001;202(2);243-50. PMID: 11520622

Stafford05: Stafford GP, Ogi T, Hughes C (2005). "Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2." Microbiology 151(Pt 6);1779-88. PMID: 15941987


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.