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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted protein

Gene: yacC Accession Numbers: EG11089 (EcoCyc), b0122, ECK0121

Regulation Summary Diagram: ?

Regulation summary diagram for yacC

yacC is cotranscribed with speED [Xie89]. A deletion upstream of speE, extending into the yacC open reading frame, leads to loss of spermidine synthase and S-adenosylmethionine decarboxylase activity [Tabor86].

Gene Citations: [Xie93]

Map Position: [136,570 <- 136,917] (2.94 centisomes, 11°)
Length: 348 bp / 115 aa

Molecular Weight of Polypeptide: 12.854 kD (from nucleotide sequence), 12 kD (experimental) [Xie89 ]

Unification Links: ASAP:ABE-0000423 , EchoBASE:EB1081 , EcoGene:EG11089 , EcoliWiki:b0122 , OU-Microarray:b0122 , PortEco:yacC , Protein Model Portal:P0AA95 , RefSeq:NP_414664 , RegulonDB:EG11089 , SMR:P0AA95 , String:511145.b0122 , UniProt:P0AA95

Relationship Links: InterPro:IN-FAMILY:IPR019114 , Pfam:IN-FAMILY:PF09691

Gene Class: ORFs

Essentiality data for yacC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Last-Curated ? 01-Aug-2006 by Keseler I , SRI International

Sequence Features

Protein sequence of predicted protein with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 20
UniProt: Non-Experimental Qualifier: potential;
Chain 21 -> 115
UniProt: Uncharacterized protein yacC;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b0122 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11089; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Tabor86: Tabor CW, Tabor H, Xie QW (1986). "Spermidine synthase of Escherichia coli: localization of the speE gene." Proc Natl Acad Sci U S A 83(16);6040-4. PMID: 3526348

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

Xie89: Xie QW, Tabor CW, Tabor H (1989). "Spermidine biosynthesis in Escherichia coli: promoter and termination regions of the speED operon." J Bacteriol 1989;171(8);4457-65. PMID: 2666401

Xie93: Xie QW, Tabor CW, Tabor H (1993). "Deletion mutations in the speED operon: spermidine is not essential for the growth of Escherichia coli." Gene 1993;126(1);115-7. PMID: 8472951

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Sep 2, 2015, biocyc13.