Escherichia coli K-12 substr. MG1655 Enzyme: D-mannonate dehydratase

Gene: uxuA Accession Numbers: EG11066 (EcoCyc), b4322, ECK4313

Regulation Summary Diagram: ?

Regulation summary diagram for uxuA

D-mannonate dehydratase catalyzes the final reaction of the glucuronate branch of the hexuronate degradation pathway [Portalier80].

UxuA is monomeric in solution. Crystal structures of the enzyme alone and in complex with D-mannonate have been solved [Qiu12].

The enzyme is inducible by glucuronate and fructuronate [RobertBaudouy73]. Expression of uxuA may be repressed by OxyR [Zheng01].

UxuA: "utilization of hexuronate A"

Citations: [RobertBaudouy81]

Gene Citations: [Blanco86]

Locations: cytosol

Map Position: [4,549,659 -> 4,550,843] (98.06 centisomes, 353°)
Length: 1185 bp / 394 aa

Molecular Weight of Polypeptide: 44.838 kD (from nucleotide sequence), 40.0 kD (experimental) [Ritzenthaler80 ]

pI: 4.53

Unification Links: ASAP:ABE-0014173 , CGSC:14 , DIP:DIP-11108N , EchoBASE:EB1059 , EcoGene:EG11066 , EcoliWiki:b4322 , ModBase:P24215 , OU-Microarray:b4322 , PortEco:uxuA , PR:PRO_000024206 , Pride:P24215 , Protein Model Portal:P24215 , RefSeq:NP_418742 , RegulonDB:EG11066 , SMR:P24215 , String:511145.b4322 , UniProt:P24215

Relationship Links: InterPro:IN-FAMILY:IPR004628 , InterPro:IN-FAMILY:IPR013022 , PDB:Structure:4EAC , PDB:Structure:4EAY , Pfam:IN-FAMILY:PF03786

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for uxuA

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0042840 - D-glucuronate catabolic process Inferred from experiment [RobertBaudouy72, Portalier80]
GO:0006064 - glucuronate catabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0008198 - ferrous iron binding Inferred from experiment [RobertBaudouy73, Dreyer87]
GO:0008927 - mannonate dehydratase activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01, GOA01a, Ritzenthaler80, RobertBaudouy73, Dreyer87]
GO:0030145 - manganese ion binding Inferred from experiment [Qiu12]
GO:0016829 - lyase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds

Essentiality data for uxuA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Last-Curated ? 16-Aug-2012 by Keseler I , SRI International

Enzymatic reaction of: D-mannonate dehydratase

Synonyms: D-mannonate hydro-lyase, mannonate hydrolase, mannonate hydratase, ManD

EC Number:

D-mannonate <=> 2-dehydro-3-deoxy-D-gluconate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is irreversible in the direction shown.

Alternative Substrates [Comment 5]:

In Pathways: superpathway of hexuronide and hexuronate degradation , superpathway of β-D-glucuronide and D-glucuronate degradation , D-fructuronate degradation

Inactivation of the enzyme by p-chloromercuribenzoate was reported by [RobertBaudouy73], but no inhibition was seen by [Dreyer87].

The enzyme was first partially purified from E. coli W [Smiley60].

Activators (Unknown Mechanism): Fe2+ [RobertBaudouy73, Dreyer87] , Mn2+ [RobertBaudouy73, Dreyer87] , D-glucuronate [RobertBaudouy73] , D-galacturonate [RobertBaudouy73] , D-tagaturonate [RobertBaudouy73] , D-altronate [RobertBaudouy73]

Inhibitors (Competitive): D-sorbitol [RobertBaudouy73] , D-mannitol [RobertBaudouy73] , D-gluconate [RobertBaudouy73]

Inhibitors (Noncompetitive): glucuronamide [RobertBaudouy73] , mannonic amide [RobertBaudouy73]

Inhibitors (Unknown Mechanism): o-phenanthroline [Dreyer87] , p-chloromercuribenzoate [RobertBaudouy73]

Kinetic Parameters:

Km (μM)
[RobertBaudouy73, BRENDA14]

pH(opt) (forward direction): 7.5 [Dreyer87]

pH(opt): 7 [BRENDA14, RobertBaudouy82], 8.3 [BRENDA14, RobertBaudouy73]

Sequence Features

Protein sequence of D-mannonate dehydratase with features indicated

Feature Class Location Citations Comment
Extrinsic-Sequence-Variant 52
UniProt: In strain: MT78..

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b4322 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11066; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Blanco86: Blanco C, Ritzenthaler P, Kolb A (1986). "The regulatory region of the uxuAB operon in Escherichia coli K12." Mol Gen Genet 1986;202(1);112-9. PMID: 3083215

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014."

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dreyer87: Dreyer JL (1987). "The role of iron in the activation of mannonic and altronic acid hydratases, two Fe-requiring hydro-lyases." Eur J Biochem 166(3);623-30. PMID: 3038546

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

Portalier80: Portalier R, Robert-Baudouy J, Stoeber F (1980). "Regulation of Escherichia coli K-12 hexuronate system genes: exu regulon." J Bacteriol 143(3);1095-107. PMID: 6997263

Qiu12: Qiu X, Tao Y, Zhu Y, Yuan Y, Zhang Y, Liu H, Gao Y, Teng M, Niu L (2012). "Structural insights into decreased enzymatic activity induced by an insert sequence in mannonate dehydratase from Gram negative bacterium." J Struct Biol. PMID: 22796868

Ritzenthaler80: Ritzenthaler P, Mata-Gilsinger M, Stoeber F (1980). "Construction and expression of hybrid plasmids containing Escherichia coli K-12 uxu genes." J Bacteriol 143(3);1116-26. PMID: 6997264

RobertBaudouy72: Robert-Baudouy JM, Portalier RC, Stoeber FR (1972). "[Genetic mapping and biochemical characterization of mutations in the mannonic hydrolyase structural gene of Escherichia coli K 12]." Mol Gen Genet 118(4);351-62. PMID: 4570161

RobertBaudouy73: Robert-Baudouy JM, Stoeber FR (1973). "[Purification and properties of D-mannonate hydrolyase from Escherichia coli K12]." Biochim Biophys Acta 1973;309(2);473-85. PMID: 4581499

RobertBaudouy81: Robert-Baudouy J, Portalier R, Stoeber F (1981). "Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products." J Bacteriol 145(1);211-20. PMID: 7007313

RobertBaudouy82: Robert-Baudouy J, Jimeno-Abendano J, Stoeber F (1982). "D-Mannonate and D-altronate dehydratases of Escherichia coli K12." Methods Enzymol 1982;90 Pt E;288-94. PMID: 6759855

Smiley60: Smiley JD, Ashwell G (1960). "Uronic acid metabolism in bacteria. III. Purification and properties of D-altronic acid and D-mannonic acid dehydrases in Escherichia coli." J Biol Chem 235;1571-5. PMID: 13831814

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zheng01: Zheng M, Wang X, Templeton LJ, Smulski DR, LaRossa RA, Storz G (2001). "DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide." J Bacteriol 183(15);4562-70. PMID: 11443091

Other References Related to Gene Regulation

Bates04: Bates Utz C, Nguyen AB, Smalley DJ, Anderson AB, Conway T (2004). "GntP is the Escherichia coli Fructuronic acid transporter and belongs to the UxuR regulon." J Bacteriol 186(22);7690-6. PMID: 15516583

Klemm96: Klemm P, Tong S, Nielsen H, Conway T (1996). "The gntP gene of Escherichia coli involved in gluconate uptake." J Bacteriol 178(1);61-7. PMID: 8550444

Lynch96: Lynch AS, Lin EC (1996). "Transcriptional control mediated by the ArcA two-component response regulator protein of Escherichia coli: characterization of DNA binding at target promoters." J Bacteriol 1996;178(21);6238-49. PMID: 8892825

Ritzenthaler82: Ritzenthaler P, Mata-Gilsinger M (1982). "Use of in vitro gene fusions to study the uxuR regulatory gene in Escherichia coli K-12: direction of transcription and regulation of its expression." J Bacteriol 150(3);1040-7. PMID: 6281232

Ritzenthaler85: Ritzenthaler P, Blanco C, Mata-Gilsinger M (1985). "Genetic analysis of uxuR and exuR genes: evidence for ExuR and UxuR monomer repressors interactions." Mol Gen Genet 199(3);507-11. PMID: 3929016

Rodionov00: Rodionov DA, Mironov AA, Rakhmaninova AB, Gelfand MS (2000). "Transcriptional regulation of transport and utilization systems for hexuronides, hexuronates and hexonates in gamma purple bacteria." Mol Microbiol 38(4);673-83. PMID: 11115104

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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