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discounted EARLY registration ends Dec 31, 2014
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted semialdehyde dehydrogenase



Gene: usg Accession Numbers: EG11059 (EcoCyc), b2319, ECK2313

Synonyms: usg-1

Regulation Summary Diagram: ?

Summary:
Usg shows some similarity to aspartate β-semialdehyde dehydrogenase [Henikoff88]. The Usg protein has been purified [Marvel89].

A transposon insertion in usg has a polar effect on hisT [Arps87].

Usg: "upstream gene" [Arps85]

Gene Citations: [Schoenlein89, Arps87a, Pease02]

Locations: cytosol

Map Position: [2,433,658 <- 2,434,671] (52.45 centisomes)
Length: 1014 bp / 337 aa

Molecular Weight of Polypeptide: 36.364 kD (from nucleotide sequence), 45.0 kD (experimental) [Marvel85 ]

Unification Links: ASAP:ABE-0007660 , DIP:DIP-11095N , EchoBASE:EB1052 , EcoGene:EG11059 , EcoliWiki:b2319 , Mint:MINT-1222362 , ModBase:P08390 , OU-Microarray:b2319 , PortEco:usg , PR:PRO_000024188 , Pride:P08390 , Protein Model Portal:P08390 , RefSeq:NP_416822 , RegulonDB:EG11059 , SMR:P08390 , String:511145.b2319 , UniProt:P08390

Relationship Links: InterPro:IN-FAMILY:IPR000534 , InterPro:IN-FAMILY:IPR012080 , InterPro:IN-FAMILY:IPR012280 , InterPro:IN-FAMILY:IPR016040 , Pfam:IN-FAMILY:PF01118 , Pfam:IN-FAMILY:PF02774 , Smart:IN-FAMILY:SM00859

GO Terms:

Biological Process: GO:0008652 - cellular amino acid biosynthetic process Inferred by computational analysis [GOA01a]
GO:0009086 - methionine biosynthetic process Inferred by computational analysis [GOA01a]
GO:0009088 - threonine biosynthetic process Inferred by computational analysis [GOA01a]
GO:0009089 - lysine biosynthetic process via diaminopimelate Inferred by computational analysis [GOA01a]
GO:0009097 - isoleucine biosynthetic process Inferred by computational analysis [GOA01a]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0003942 - N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred by computational analysis [GOA01a]
GO:0004073 - aspartate-semialdehyde dehydrogenase activity Inferred by computational analysis [GOA01a]
GO:0016620 - oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred by computational analysis [GOA01a]
GO:0046983 - protein dimerization activity Inferred by computational analysis [GOA01a]
GO:0050661 - NADP binding Inferred by computational analysis [GOA01a]
GO:0051287 - NAD binding Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, LopezCampistrou05]
GO:0005737 - cytoplasm Inferred by computational analysis [GOA01a]

Gene Class: ORFs

Essentiality data for usg knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]

Credits:
Last-Curated ? 27-Feb-2006 by Keseler I , SRI International


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
1/26/1998 (pkarp) Merged genes G7988/usg and EG11059/usg


References

Arps85: Arps PJ, Marvel CC, Rubin BC, Tolan DA, Penhoet EE, Winkler ME (1985). "Structural features of the hisT operon of Escherichia coli K-12." Nucleic Acids Res 13(14);5297-315. PMID: 2991861

Arps87: Arps PJ, Winkler ME (1987). "Structural analysis of the Escherichia coli K-12 hisT operon by using a kanamycin resistance cassette." J Bacteriol 169(3);1061-70. PMID: 3029016

Arps87a: Arps PJ, Winkler ME (1987). "An unusual genetic link between vitamin B6 biosynthesis and tRNA pseudouridine modification in Escherichia coli K-12." J Bacteriol 1987;169(3);1071-9. PMID: 3029017

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Henikoff88: Henikoff S, Wallace JC (1988). "Detection of protein similarities using nucleotide sequence databases." Nucleic Acids Res 16(13);6191-204. PMID: 3135536

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Marvel85: Marvel CC, Arps PJ, Rubin BC, Kammen HO, Penhoet EE, Winkler ME (1985). "hisT is part of a multigene operon in Escherichia coli K-12." J Bacteriol 161(1);60-71. PMID: 2981810

Marvel89: Marvel CC, Kammen HO (1989). "Purification of plasmid-expressed proteins which lack functional assay systems." Anal Biochem 181(2);336-40. PMID: 2683865

Pease02: Pease AJ, Roa BR, Luo W, Winkler ME (2002). "Positive growth rate-dependent regulation of the pdxA, ksgA, and pdxB genes of Escherichia coli K-12." J Bacteriol 184(5);1359-69. PMID: 11844765

Schoenlein89: Schoenlein PV, Roa BB, Winkler ME (1989). "Divergent transcription of pdxB and homology between the pdxB and serA gene products in Escherichia coli K-12." J Bacteriol 171(11);6084-92. PMID: 2681152


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14B.