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Escherichia coli K-12 substr. MG1655 Transporter: nucleoside:H+ symporter NupG



Gene: nupG Accession Numbers: EG10664 (EcoCyc), b2964, ECK2959

Synonyms: NupG nucleoside MFS transporter

Regulation Summary Diagram: ?

Summary:
NupG is one of two high-affinity nucleoside transporters in E. coli. Studies of chromosomal mutants using membrane vesicles have shown that NupG differs from NupC by mediating transport of all nucleosides whereas NupC does not transport guanosine or deoxyguanosine [Komatsu73, MunchPetersen79]. The cloned nupG gene has been demonstrated to complement nupG chromosomal mutations [Westh87]. NupG is a member of the major facilitator superfamily (MFS) of transporters [Pao98] and presumably functions as a nucleoside/proton symporter. Analysis of nupG-lacZ fusions has shown that expression of nupG is regulated by the CytR and DeoR transcriptional regulators. Imported nucleosides serve as precursors of DNA and RNA, as well as of histidine and various co-factors.

Purification studies showed that recombinant NupG transported purine (adenosine) and pyrimidine (uridine) nucleosides with apparent K(m) values of approximately 20-30 uM. Transport was energized primarily by the proton motive force (pmf) across the cell membrane [Xie04].

Gene Citations: [Gifford99, Li94a, Pedersen95]

Locations: inner membrane

Map Position: [3,103,736 -> 3,104,992] (66.9 centisomes)
Length: 1257 bp / 418 aa

Molecular Weight of Polypeptide: 46.389 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009728 , CGSC:442 , EchoBASE:EB0658 , EcoGene:EG10664 , EcoliWiki:b2964 , ModBase:P0AFF4 , OU-Microarray:b2964 , PortEco:nupG , PR:PRO_000023443 , Protein Model Portal:P0AFF4 , RefSeq:NP_417439 , RegulonDB:EG10664 , String:511145.b2964 , UniProt:P0AFF4

Relationship Links: InterPro:IN-FAMILY:IPR004740 , InterPro:IN-FAMILY:IPR016196 , InterPro:IN-FAMILY:IPR020846 , Pfam:IN-FAMILY:PF03825 , Prosite:IN-FAMILY:PS50850

In Paralogous Gene Group: 26 (5 members)

Gene-Reaction Schematic: ?

Instance reactions of [a nucleoside[periplasmic space] + H+[periplasmic space] → a nucleoside[cytosol] + H+[cytosol]] (no EC#):
i1: uridine[periplasmic space] + H+[periplasmic space] → uridine[cytosol] + H+[cytosol] (no EC#)

i2: thymidine[periplasmic space] + H+[periplasmic space] → thymidine[cytosol] + H+[cytosol] (no EC#)

i3: inosine[periplasmic space] + H+[periplasmic space] → inosine[cytosol] + H+[cytosol] (no EC#)

i4: 2'-deoxyuridine[periplasmic space] + H+[periplasmic space] → 2'-deoxyuridine[cytosol] + H+[cytosol] (no EC#)

i5: 2'-deoxyinosine[periplasmic space] + H+[periplasmic space] → 2'-deoxyinosine[cytosol] + H+[cytosol] (no EC#)

i6: 2'-deoxycytidine[periplasmic space] + H+[periplasmic space] → 2'-deoxycytidine[cytosol] + H+[cytosol] (no EC#)

i7: 2'-deoxyadenosine[periplasmic space] + H+[periplasmic space] → 2'-deoxyadenosine[cytosol] + H+[cytosol] (no EC#)

i8: cytidine[periplasmic space] + H+[periplasmic space] → cytidine[cytosol] + H+[cytosol] (no EC#)

i9: adenosine[periplasmic space] + H+[periplasmic space] → adenosine[cytosol] + H+[cytosol] (no EC#)

i10: xanthosine[periplasmic space] + H+[periplasmic space] → xanthosine[cytosol] + H+[cytosol] (no EC#)

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0015860 - purine nucleoside transmembrane transport Inferred from experiment [MunchPetersen79]
GO:0015861 - cytidine transport Inferred from experiment [MunchPetersen79]
GO:0015862 - uridine transport Inferred from experiment [Xie04]
GO:0015864 - pyrimidine nucleoside transport Inferred from experiment [Xie04]
GO:0032238 - adenosine transport Inferred from experiment [MunchPetersen79]
GO:0072531 - pyrimidine-containing compound transmembrane transport Inferred from experiment [Xie04]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0015858 - nucleoside transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015212 - cytidine transmembrane transporter activity Inferred from experiment [MunchPetersen79]
GO:0015213 - uridine transmembrane transporter activity Inferred from experiment [MunchPetersen79, Xie04]
GO:0015214 - pyrimidine nucleoside transmembrane transporter activity Inferred from experiment [Xie04]
GO:0005337 - nucleoside transmembrane transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Xie04]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
metabolism central intermediary metabolism nucleotide and nucleoside conversions
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for nupG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: transport of a nucleoside (nucleoside:H+ symporter NupG)


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 9 -> 29
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 40 -> 60
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 70 -> 90
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 96 -> 116
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 137 -> 157
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 162 -> 182
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 210 -> 230
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 255 -> 275
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 283 -> 303
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 305 -> 325
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 349 -> 369
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 382 -> 402
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
10/20/97 Gene b2964 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10664; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Gifford99: Gifford CM, Wallace SS (1999). "The genes encoding formamidopyrimidine and MutY DNA glycosylases in Escherichia coli are transcribed as part of complex operons." J Bacteriol 181(14);4223-36. PMID: 10400579

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Komatsu73: Komatsu Y, Tanaka K (1973). "Deoxycytidine uptake by isolated membrane vesicles from Escherichia coli K 12." Biochim Biophys Acta 1973;311(4);496-506. PMID: 4354132

Kreis99: Kreis R, Jung B, Slotboom J, Felblinger J, Boesch C (1999). "Effect of exercise on the creatine resonances in 1H MR spectra of human skeletal muscle." J Magn Reson 137(2);350-7. PMID: 10089169

Li94a: Li J, Kustu S, Stewart V (1994). "In vitro interaction of nitrate-responsive regulatory protein NarL with DNA target sequences in the fdnG, narG, narK and frdA operon control regions of Escherichia coli K-12." J Mol Biol 1994;241(2);150-65. PMID: 8057356

MunchPetersen79: Munch-Petersen A, Mygind B, Nicolaisen A, Pihl NJ (1979). "Nucleoside transport in cells and membrane vesicles from Escherichia coli K12." J Biol Chem 1979;254(10);3730-7. PMID: 374403

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

Pedersen95: Pedersen H, Dall J, Dandanell G, Valentin-Hansen P (1995). "Gene-regulatory modules in Escherichia coli: nucleoprotein complexes formed by cAMP-CRP and CytR at the nupG promoter." Mol Microbiol 1995;17(5);843-53. PMID: 8596434

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Westh87: Westh Hansen SE, Jensen N, Munch-Petersen A (1987). "Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system." Eur J Biochem 1987;168(2);385-91. PMID: 3311747

Xie04: Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SY, Ng AM, Young JD, Herbert RB, Henderson PJ, Baldwin SA (2004). "Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family." Mol Membr Biol 21(5);323-36. PMID: 15513740

Other References Related to Gene Regulation

Gavigan99: Gavigan SA, Nguyen T, Nguyen N, Senear DF (1999). "Role of multiple CytR binding sites on cooperativity, competition, and induction at the Escherichia coli udp promoter." J Biol Chem 1999;274(23);16010-9. PMID: 10347150

Holst92: Holst B, Sogaard-Andersen L, Pedersen H, Valentin-Hansen P (1992). "The cAMP-CRP/CytR nucleoprotein complex in Escherichia coli: two pairs of closely linked binding sites for the cAMP-CRP activator complex are involved in combinatorial regulation of the cdd promoter." EMBO J 11(10);3635-43. PMID: 1327747

Holt10: Holt AK, Senear DF (2010). "An unusual pattern of CytR and CRP binding energetics at Escherichia coli cddP suggests a unique blend of class I and class II mediated activation." Biochemistry 49(3);432-42. PMID: 20000490

Jorgensen98: Jorgensen CI, Kallipolitis BH, Valentin-Hansen P (1998). "DNA-binding characteristics of the Escherichia coli CytR regulator: a relaxed spacing requirement between operator half-sites is provided by a flexible, unstructured interdomain linker." Mol Microbiol 27(1);41-50. PMID: 9466254

Meibom00: Meibom KL, Kallipolitis BH, Ebright RH, Valentin-Hansen P (2000). "Identification of the subunit of cAMP receptor protein (CRP) that functionally interacts with CytR in CRP-CytR-mediated transcriptional repression." J Biol Chem 275(16);11951-6. PMID: 10766824

MunchPetersen90: Munch-Petersen A, Jensen N (1990). "Analysis of the regulatory region of the Escherichia coli nupG gene, encoding a nucleoside-transport protein." Eur J Biochem 1990;190(3);547-51. PMID: 2115441

Otsuka96: Otsuka J, Watanabe H, Mori KT (1996). "Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli." J Theor Biol 1996;178(2);183-204. PMID: 8729576

Pedersen97: Pedersen H, Valentin-Hansen P (1997). "Protein-induced fit: the CRP activator protein changes sequence-specific DNA recognition by the CytR repressor, a highly flexible LacI member." EMBO J 16(8);2108-18. PMID: 9155036

Perini96: Perini LT, Doherty EA, Werner E, Senear DF (1996). "Multiple specific CytR binding sites at the Escherichia coli deoP2 promoter mediate both cooperative and competitive interactions between CytR and cAMP receptor protein." J Biol Chem 271(52);33242-55. PMID: 8969182

SogaardAndersen90: Sogaard-Andersen L, Martinussen J, Mollegaard NE, Douthwaite SR, Valentin-Hansen P (1990). "The CytR repressor antagonizes cyclic AMP-cyclic AMP receptor protein activation of the deoCp2 promoter of Escherichia coli K-12." J Bacteriol 1990;172(10);5706-13. PMID: 2170326

SogaardAndersen90a: Sogaard-Andersen L, Mollegaard NE, Douthwaite SR, Valentin-Hansen P (1990). "Tandem DNA-bound cAMP-CRP complexes are required for transcriptional repression of the deoP2 promoter by the CytR repressor in Escherichia coli." Mol Microbiol 4(9);1595-601. PMID: 1962841

SogaardAndersen91b: Sogaard-Andersen L, Pedersen H, Holst B, Valentin-Hansen P (1991). "A novel function of the cAMP-CRP complex in Escherichia coli: cAMP-CRP functions as an adaptor for the CytR repressor in the deo operon." Mol Microbiol 5(4);969-75. PMID: 1649947

ValentinHansen96: Valentin-Hansen P, Sogaard-Andersen L, Pedersen H (1996). "A flexible partnership: the CytR anti-activator and the cAMP-CRP activator protein, comrades in transcription control." Mol Microbiol 20(3);461-6. PMID: 8736525

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470

Zolotukhina02: Zolotukhina MA, Ovcharova IV, Eremina SIu, Errais LL, Mironov AS (2002). "[Functional interrelationship between elements of the Escherichia coli udp gene promotor responsible for binding regulatory proteins CytR, CRP, and RNA polymerase]." Genetika 38(9);1223-34. PMID: 12391883


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc13.