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Escherichia coli K-12 substr. MG1655 Polypeptide: regulator for fec operon, periplasmic



Gene: fecR Accession Numbers: EG10292 (EcoCyc), b4292, ECK4282

Regulation Summary Diagram: ?

Component of: FecAR Signal Transduction System (summary available)

Gene Citations: [Enz95, Arechaga03]

Locations: periplasmic space

Map Position: [4,514,787 <- 4,515,740] (97.31 centisomes)
Length: 954 bp / 317 aa

Molecular Weight of Polypeptide: 35.532 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014070 , CGSC:35627 , DIP:DIP-9589N , EchoBASE:EB0288 , EcoGene:EG10292 , EcoliWiki:b4292 , Mint:MINT-119295 , OU-Microarray:b4292 , PortEco:fecR , PR:PRO_000022596 , Protein Model Portal:P23485 , RefSeq:NP_418712 , RegulonDB:EG10292 , String:511145.b4292 , UniProt:P23485

Relationship Links: InterPro:IN-FAMILY:IPR006860 , InterPro:IN-FAMILY:IPR012373 , Panther:IN-FAMILY:PTHR30273 , Pfam:IN-FAMILY:PF04773

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11]
GO:0050896 - response to stimulus Inferred by computational analysis [UniProtGOA11]
GO:0055072 - iron ion homeostasis Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: cell processes adaptations Fe aquisition
information transfer RNA related Transcription related
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)
regulation type of regulation transcriptional level sigma factors, anti-sigmafactors

Essentiality data for fecR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: FecAR Signal Transduction System

Subunit composition of FecAR Signal Transduction System = [FecA][FecR]
         ferric citrate outer membrane porin FecA = FecA (summary available)
         regulator for fec operon, periplasmic = FecR

Summary:
FecARI are responsible for ferric citrate dependent induction of the ferric citrate uptake system. When the inducer, ferric citrate, binds FecA, which is also responsible for transport of ferric citrate across the outer membrane, a signal is transmitted across the outer membrane to the cytoplasmic membrane protein FecR. FecR transmits the signal across the cytoplasmic membrane and activates the σ70 family protein FecI in the cytoplasm. FecI directs RNA polymerase to express fecABCDE, which encodes the ferric citrate outer membrane receptor and the ferric citrate ABC transporter, as well as fecIR [Harle95, Enz00]. Expression of fecIR is repressed by Fe2+ bound to Fur [Braun05].

Reviews: [Braun02, Braun03, Braun05, Braun06]


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b4292 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10292; confirmed by SwissProt match.


References

Arechaga03: Arechaga I, Miroux B, Runswick MJ, Walker JE (2003). "Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript." FEBS Lett 547(1-3);97-100. PMID: 12860393

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Braun02: Braun V, Braun M (2002). "Iron transport and signaling in Escherichia coli." FEBS Lett 529(1);78-85. PMID: 12354617

Braun03: Braun V, Mahren S, Ogierman M (2003). "Regulation of the FecI-type ECF sigma factor by transmembrane signalling." Curr Opin Microbiol 6(2);173-80. PMID: 12732308

Braun05: Braun V, Mahren S, Sauter A (2005). "Gene regulation by transmembrane signaling." Biometals 18(5);507-17. PMID: 16333751

Braun06: Braun V, Mahren S, Sauter A (2006). "Gene regulation by transmembrane signaling." Biometals 19(2);103-13. PMID: 16718597

Enz00: Enz S, Mahren S, Stroeher UH, Braun V (2000). "Surface signaling in ferric citrate transport gene induction: interaction of the FecA, FecR, and FecI regulatory proteins." J Bacteriol 182(3);637-46. PMID: 10633096

Enz95: Enz S, Braun V, Crosa JH (1995). "Transcription of the region encoding the ferric dicitrate-transport system in Escherichia coli: similarity between promoters for fecA and for extracytoplasmic function sigma factors." Gene 163(1);13-8. PMID: 7557462

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Harle95: Harle C, Kim I, Angerer A, Braun V (1995). "Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface." EMBO J 14(7);1430-8. PMID: 7729419

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Angerer98: Angerer A, Braun V (1998). "Iron regulates transcription of the Escherichia coli ferric citrate transport genes directly and through the transcription initiation proteins." Arch Microbiol 1998;169(6);483-90. PMID: 9575233

Chen07: Chen Z, Lewis KA, Shultzaberger RK, Lyakhov IG, Zheng M, Doan B, Storz G, Schneider TD (2007). "Discovery of Fur binding site clusters in Escherichia coli by information theory models." Nucleic Acids Res 35(20);6762-77. PMID: 17921503

Ochs96: Ochs M, Angerer A, Enz S, Braun V (1996). "Surface signaling in transcriptional regulation of the ferric citrate transport system of Escherichia coli: mutational analysis of the alternative sigma factor FecI supports its essential role in fec transport gene transcription." Mol Gen Genet 250(4);455-65. PMID: 8602163

Pressler88: Pressler U, Staudenmaier H, Zimmermann L, Braun V (1988). "Genetics of the iron dicitrate transport system of Escherichia coli." J Bacteriol 170(6);2716-24. PMID: 2836368

Stiefel01: Stiefel A, Mahren S, Ochs M, Schindler PT, Enz S, Braun V (2001). "Control of the ferric citrate transport system of Escherichia coli: mutations in region 2.1 of the FecI extracytoplasmic-function sigma factor suppress mutations in the FecR transmembrane regulatory protein." J Bacteriol 183(1);162-70. PMID: 11114913


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc14.