Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Enzyme: cobinamide adenosyltransferase / cobalamin adenosyltransferase



Gene: btuR Accession Numbers: EG10130 (EcoCyc), b1270, ECK1264

Synonyms: cobA

Regulation Summary Diagram: ?

Summary:
E. coli K-12, as well as natural isolates, can synthesize cobalamin only when supplied with the intermediate cobinamide [Lawrence96]. Sequence similarity and complementation of a cobA mutant in Salmonella typhimurium suggests that btuR encodes the cobinamide adenosyltransferase [EscalanteSemere90, Suh93]. Cobinamide adenosyltransferase catalyzes the synthesis of adenosylcobalamin from a cobalamin precursor transported into the cell [Lundrigan89].

A btuR mutation leads to loss of repression of expression by externally supplied vitamin B12 and was therefore initially thought to encode a transcriptional repressor of btuB [Lundrigan87]. However, it was later shown that a btuR mutation results in a decrease in the pool of intracellular adenosylcobalamin and is thus more likely involved in the conversion of vitamin B12 to adenosylcobalamin [Lundrigan89]. Insertion mutants within btuR lead to an inability to utilize cobinamide under aerobic growth conditions [Lawrence95].

BtuR: "B twelve uptake regulation"

Locations: cytosol

Map Position: [1,325,791 <- 1,326,381] (28.58 centisomes)
Length: 591 bp / 196 aa

Molecular Weight of Polypeptide: 21.999 kD (from nucleotide sequence), 22 kD (experimental) [Lundrigan89 ]

pI: 6.47

Unification Links: ASAP:ABE-0004261 , CGSC:18493 , EchoBASE:EB0128 , EcoGene:EG10130 , EcoliWiki:b1270 , ModBase:P0A9H5 , OU-Microarray:b1270 , PortEco:btuR , PR:PRO_000022239 , Pride:P0A9H5 , Protein Model Portal:P0A9H5 , RefSeq:NP_415786 , RegulonDB:EG10130 , SMR:P0A9H5 , String:511145.b1270 , Swiss-Model:P0A9H5 , UniProt:P0A9H5

Relationship Links: InterPro:IN-FAMILY:IPR003724 , InterPro:IN-FAMILY:IPR025826 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF02572 , Pfam:IN-FAMILY:PF12557

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009236 - cobalamin biosynthetic process Inferred from experiment Inferred by computational analysis [UniProtGOA12, UniProtGOA11a, GOA01, Lawrence95, Lundrigan89]
GO:0019250 - aerobic cobalamin biosynthetic process Inferred from experiment [Lawrence95]
GO:0006779 - porphyrin-containing compound biosynthetic process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0008817 - cob(I)yrinic acid a,c-diamide adenosyltransferase activity Inferred by computational analysis [GOA01a, GOA01, Suh93]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism biosynthesis of building blocks cofactors, small molecule carriers cobalamin

Essentiality data for btuR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Credits:
Last-Curated ? 23-May-2012 by Keseler I , SRI International


Enzymatic reaction of: cobinamide adenosyltransferase

EC Number: 2.5.1.17

cobinamide + ATP <=> adenosylcobinamide + PPPi

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

In Pathways: adenosylcobalamin salvage from cobinamide I


Enzymatic reaction of: cobalamin adenosyltransferase

Synonyms: cob(I)alamin adenosyltransferase, corrinoid adenosyltransferase, aquacob(I)alamin adenosyltransferase, ATP:cob(I)alamin Co-β-adenosyltransferase

EC Number: 2.5.1.17

ATP + cob(I)alamin <=> coenzyme B12 + PPPi

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

In Pathways: adenosylcobalamin salvage from cobalamin


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 36 -> 42
[UniProt10]
UniProt: ATP; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b1270 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10130; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

EscalanteSemere90: Escalante-Semerena JC, Suh SJ, Roth JR (1990). "cobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella typhimurium." J Bacteriol 172(1);273-80. PMID: 2403541

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lawrence95: Lawrence JG, Roth JR (1995). "The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli." J Bacteriol 1995;177(22);6371-80. PMID: 7592411

Lawrence96: Lawrence JG, Roth JR (1996). "Evolution of coenzyme B12 synthesis among enteric bacteria: evidence for loss and reacquisition of a multigene complex." Genetics 142(1);11-24. PMID: 8770581

Lundrigan87: Lundrigan MD, De Veaux LC, Mann BJ, Kadner RJ (1987). "Separate regulatory systems for the repression of metE and btuB by vitamin B12 in Escherichia coli." Mol Gen Genet 206(3);401-7. PMID: 3108627

Lundrigan89: Lundrigan MD, Kadner RJ (1989). "Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation." J Bacteriol 1989;171(1);154-61. PMID: 2644187

Suh93: Suh SJ, Escalante-Semerena JC (1993). "Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium." Gene 129(1);93-7. PMID: 7916712

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC13A.