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Escherichia coli K-12 substr. MG1655 Polypeptide: YnjD



Gene: ynjD Accession Numbers: G6951 (EcoCyc), b1756, ECK1754

Regulation Summary Diagram: ?

Component of: YnjC/YnjD ABC transporter (summary available)

Summary:
YnjD is an ATP-binding component of a predicted metabolite uptake ABC transporter.

Locations: inner membrane, cytosol

Map Position: [1,836,771 -> 1,837,424] (39.59 centisomes)
Length: 654 bp / 217 aa

Molecular Weight of Polypeptide: 24.017 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005845 , EchoBASE:EB3762 , EcoGene:EG14006 , EcoliWiki:b1756 , ModBase:P76909 , OU-Microarray:b1756 , PortEco:ynjD , Pride:P76909 , Protein Model Portal:P76909 , RefSeq:NP_416270 , RegulonDB:G6951 , SMR:P76909 , String:511145.b1756 , UniProt:P76909

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for ynjD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of: YnjC/YnjD ABC transporter

Subunit composition of YnjC/YnjD ABC transporter = [YnjC]2[YnjD]2

Summary:
YnjD and YnjC are uncharacterized members of the ABC superfamily of transporters [Saurin99]. YnjD is the putative ATP-binding component. YnjC is the putative membrane component. No periplasmic binding component has yet been identified. Based on sequence similiarity, these proteins may function together as an ATP-dependent metabolite uptake transporter. The genes ynjD and ynjC are probably located within a single operon.


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 2 -> 216
[UniProt09]
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 34 -> 41
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, biocyc14.