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Escherichia coli K-12 substr. MG1655 Polypeptide: aliphatic sulfonate ABC transporter - ATP binding subunit



Gene: ssuB Accession Numbers: EG12358 (EcoCyc), b0933, ECK0924

Synonyms: ycbE

Regulation Summary Diagram: ?

Component of: aliphatic sulfonate ABC transporter (extended summary available)

Summary:
Based on sequence analysis ssuB encodes the ATP-binding component of an aliphatic sulfonate ABC transporter [vanDer99]. An ΔssuB ΔssuC strain is unable to grow using a range of aliphatic sulfonates as sulfur source including MOPS, HEPES, ethanesulfonate, hexanesulfonate, octanesulfonate, decanesulfonate and sulfoacetate. Wild type growth is restored when ssuB and ssuC are expressed from a plasmid [Eichhorn00].

Locations: inner membrane, cytosol

Map Position: [992,500 <- 993,267] (21.39 centisomes)
Length: 768 bp / 255 aa

Molecular Weight of Polypeptide: 27.738 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003173 , EchoBASE:EB2261 , EcoGene:EG12358 , EcoliWiki:b0933 , ModBase:P0AAI1 , OU-Microarray:b0933 , PortEco:ssuB , PR:PRO_000023995 , Pride:P0AAI1 , Protein Model Portal:P0AAI1 , RefSeq:NP_415453 , RegulonDB:EG12358 , SMR:P0AAI1 , String:511145.b0933 , Swiss-Model:P0AAI1 , UniProt:P0AAI1

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR017875 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Prosite:IN-FAMILY:PS51291 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0042918 - alkanesulfonate transport Inferred from experiment [vanDer99, Eichhorn00]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0042959 - alkanesulfonate transporter activity Inferred from experiment [vanDer99, Eichhorn00]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005215 - transporter activity Inferred by computational analysis [GOA06, GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
GO:0042626 - ATPase activity, coupled to transmembrane movement of substances Inferred by computational analysis [vanDer99]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA01a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0031234 - extrinsic component of cytoplasmic side of plasma membrane Inferred by computational analysis [vanDer99]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [GOA06]
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing Inferred by computational analysis [vanDer99]

MultiFun Terms: metabolism metabolism of other compounds sulfur metabolism
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for ssuB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: aliphatic sulfonate ABC transporter

Synonyms: YcbE/YcbM ABC transporter, alkanesulfonate ABC transporter

Subunit composition of aliphatic sulfonate ABC transporter = [SsuA][SsuC]2[SsuB]2
         aliphatic sulfonate ABC transporter - periplasmic binding protein = SsuA (summary available)
         aliphatic sulfonate ABC transporter - membrane subunit = SsuC (summary available)
         aliphatic sulfonate ABC transporter - ATP binding subunit = SsuB (summary available)

Summary:
The ssuEADCB gene cluster codes for proteins that enable Escherichia coli to utilize a broad range of sulfonates as a source of sulfur.

A strain lacking the chromosomal ssuEADBC gene cluster is unable to use a broad range of aliphatic sulfonates as sulfur source including ethanesulfonate, propanesulfonate, butanesulfonate, pentanesulfonate, hexanesulfonate, ethanedisulfonate, octanesulfonate, decanesulfonate, isethionate, sulfoacetate, MOPS, HEPES, MES, and PIPES but is able to use sulfur from sulfate, cysteine, cystine, lanthionine, methionine, glutathione and taurine [vanDer99]. Deletion of the ssu transporter gene cluster results in the inability to use 4-phenyl-1-butanesulfonate, 2-(4-pyridyl)ethanesulfonate, 3-aminopropanesulfonate, and N-phenyltaurine as a sulfur source [Eichhorn00].

Based on sequence analysis SsuABC is an ABC type transport system with SsuA being the periplasmic substrate-binding subunit, SsuB the ATP-binding subunit, and SsuC the permease [vanDer99]. ssuD and ssuE encode an FMNH2-dependent sulfonate monooxygenase and an NAD(P)H-dependent FMN reductase, respectively.

ssu: sulfonate sulfur utilization

Locations: inner membrane

GO Terms:

Biological Process: GO:0042918 - alkanesulfonate transport Inferred from experiment [Eichhorn00, vanDer99]
Molecular Function: GO:0042959 - alkanesulfonate transporter activity Inferred from experiment [Eichhorn00, vanDer99]
GO:0042626 - ATPase activity, coupled to transmembrane movement of substances Inferred by computational analysis [vanDer99]
Cellular Component: GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing Inferred by computational analysis [vanDer99]

Credits:
Last-Curated ? 01-Jul-2014 by Mackie A , Macquarie University


Enzymatic reaction of: transport of an aliphatic sulfonate (aliphatic sulfonate ABC transporter)


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 12 -> 233
[UniProt09]
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 44 -> 51
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;
Sequence-Conflict 161 -> 165
[McCaman86, UniProt10]
Alternate sequence: GALDA → RGAGR; UniProt: (in Ref. 5; M15273);
Sequence-Conflict 232 -> 233
[McCaman86, UniProt10]
Alternate sequence: EL → DV; UniProt: (in Ref. 5; M15273);
Sequence-Conflict 236 -> 237
[McCaman86, UniProt10]
Alternate sequence: EV → RS; UniProt: (in Ref. 5; M15273);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0933 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12358; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Eichhorn00: Eichhorn E, van der Ploeg JR, Leisinger T (2000). "Deletion analysis of the Escherichia coli taurine and alkanesulfonate transport systems." J Bacteriol 182(10);2687-95. PMID: 10781534

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

McCaman86: McCaman MT, Gabe JD (1986). "The nucleotide sequence of the pepN gene and its over-expression in Escherichia coli." Gene 48(1);145-53. PMID: 3549459

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

vanDer99: van Der Ploeg JR, Iwanicka-Nowicka R, Bykowski T, Hryniewicz MM, Leisinger T (1999). "The Escherichia coli ssuEADCB gene cluster is required for the utilization of sulfur from aliphatic sulfonates and is regulated by the transcriptional activator Cbl." J Biol Chem 274(41);29358-65. PMID: 10506196

Other References Related to Gene Regulation

Bykowski02: Bykowski T, van der Ploeg JR, Iwanicka-Nowicka R, Hryniewicz MM (2002). "The switch from inorganic to organic sulphur assimilation in Escherichia coli: adenosine 5'-phosphosulphate (APS) as a signalling molecule for sulphate excess." Mol Microbiol 43(5);1347-58. PMID: 11918818

Salmon03: Salmon K, Hung SP, Mekjian K, Baldi P, Hatfield GW, Gunsalus RP (2003). "Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR." J Biol Chem 278(32);29837-55. PMID: 12754220


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc12.