Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Enzyme: sorbitol-6-phosphate dehydrogenase



Gene: srlD Accession Numbers: EG10971 (EcoCyc), b2705, ECK2700

Synonyms: gutD

Regulation Summary Diagram: ?

Subunit composition of sorbitol-6-phosphate dehydrogenase = [SrlD]4

Gene Citations: [Yamada87, McEntee77, Yamada88]

Locations: cytosol

Map Position: [2,825,759 -> 2,826,538] (60.9 centisomes)
Length: 780 bp / 259 aa

Molecular Weight of Polypeptide: 27.858 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008896 , CGSC:153 , DIP:DIP-10917N , EchoBASE:EB0964 , EcoGene:EG10971 , EcoliWiki:b2705 , ModBase:P05707 , OU-Microarray:b2705 , PortEco:srlD , PR:PRO_000023985 , Protein Model Portal:P05707 , RefSeq:NP_417185 , RegulonDB:EG10971 , SMR:P05707 , String:511145.b2705 , UniProt:P05707

Relationship Links: InterPro:IN-FAMILY:IPR002198 , InterPro:IN-FAMILY:IPR002347 , InterPro:IN-FAMILY:IPR016040 , InterPro:IN-FAMILY:IPR020904 , Pfam:IN-FAMILY:PF00106 , Prints:IN-FAMILY:PR00080 , Prints:IN-FAMILY:PR00081 , Prosite:IN-FAMILY:PS00061

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006062 - sorbitol catabolic process Inferred by computational analysis [UniProtGOA12]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0009010 - sorbitol-6-phosphate 2-dehydrogenase activity Inferred by computational analysis [GOA01a]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11, GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism carbon utilization carbon compounds

Essentiality data for srlD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: sorbitol-6-phosphate dehydrogenase

Synonyms: sorbitol-6-phosphate 2-dehydrogenase, glucitol-6-phosphate dehydrogenase, ketose phosphate reductase, D-sorbitol-6-phosphate:NAD+ 2-oxidoreductase

EC Number: 1.1.1.140

D-sorbitol 6-phosphate + NAD+ <=> D-fructose 6-phosphate + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates [Comment 5]:

In Pathways: superpathway of hexitol degradation (bacteria) , D-sorbitol degradation II

Summary:
E. coli K-12 is able to utilize sorbitol as a sole carbon source. Sorbitol is phosphorylated during transport into the cell and then converted into the glycolytic intermediate, fructose-6-phosphate, by sorbitol-6-phosphate dehydrogenase. [Novotny84, Yamada87, Lengeler75]

Inhibitors (Unknown Mechanism): N-ethylmaleimide [Novotny84] , diethylpyrocarbonate [Novotny84]

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-fructose 6-phosphate
568.0
[Roux06, BRENDA14]
NADH
25.0
[Roux06, BRENDA14]
D-sorbitol 6-phosphate
3300.0
[Novotny84, BRENDA14]
NAD+
200.0
[Novotny84, BRENDA14]

pH(opt): 8.7 [BRENDA14, Novotny84]


Sequence Features

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 4 -> 33
[UniProt10a]
UniProt: NAD; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 68
[Yamada87, UniProt10]
Alternate sequence: V → C; UniProt: (in Ref. 1; AAC13413);
Amino-Acid-Sites-That-Bind 141
[UniProt10a]
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Active-Site 154
[UniProt10a]
UniProt: Proton acceptor; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b2705 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10971; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lengeler75: Lengeler J (1975). "Mutations affecting transport of the hexitols D-mannitol, D-glucitol, and galactitol in Escherichia coli K-12: isolation and mapping." J Bacteriol 1975;124(1);26-38. PMID: 1100602

McEntee77: McEntee K (1977). "Genetic analysis of the Escherichia coli K-12 srl region." J Bacteriol 1977;132(3);904-11. PMID: 336611

Novotny84: Novotny MJ, Reizer J, Esch F, Saier MH (1984). "Purification and properties of D-mannitol-1-phosphate dehydrogenase and D-glucitol-6-phosphate dehydrogenase from Escherichia coli." J Bacteriol 1984;159(3);986-90. PMID: 6384188

Roux06: Roux C, Salmon L, Verchere-Beaur C (2006). "Preliminary studies on the inhibition of D-sorbitol-6-phosphate 2-dehydrogenase from Escherichia coli with substrate analogues." J Enzyme Inhib Med Chem 21(2);187-92. PMID: 16791965

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Yamada87: Yamada M, Saier MH (1987). "Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. Nucleotide sequence of the gut operon." J Biol Chem 1987;262(12);5455-63. PMID: 3553176

Yamada88: Yamada M, Saier MH (1988). "Positive and negative regulators for glucitol (gut) operon expression in Escherichia coli." J Mol Biol 1988;203(3);569-83. PMID: 3062173

Other References Related to Gene Regulation

Beisel11: Beisel CL, Storz G (2011). "The base-pairing RNA spot 42 participates in a multioutput feedforward loop to help enact catabolite repression in Escherichia coli." Mol Cell 41(3);286-97. PMID: 21292161

WhiteZiegler07: White-Ziegler CA, Malhowski AJ, Young S (2007). "Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12." J Bacteriol 189(15);5429-40. PMID: 17526711

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc11.