Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: RstB sensory histidine kinase



Gene: rstB Accession Numbers: EG11233 (EcoCyc), b1609, ECK1604

Synonyms: uspT

Regulation Summary Diagram: ?

Alternative forms of RstB sensory histidine kinase: RstB sensory histidine kinase - phosphorylated (summary available)

Summary:
RstB is the sensory histidine kinase of the RstBA two-component signal transduction system [Mizuno97, Yamamoto05]. rstB and rstA - encoding its cognate response regulator - are transcribed together in an operon that is induced under low Mg2+ growth conditions through the PhoPQ two-component system [Minagawa03]

Locations: inner membrane

Map Position: [1,680,906 -> 1,682,207] (36.23 centisomes)
Length: 1302 bp / 433 aa

Molecular Weight of Polypeptide: 49.283 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005372 , DIP:DIP-10808N , EchoBASE:EB1215 , EcoGene:EG11233 , EcoliWiki:b1609 , ModBase:P18392 , OU-Microarray:b1609 , PortEco:rstB , PR:PRO_000023890 , Pride:P18392 , Protein Model Portal:P18392 , RegulonDB:EG11233 , SMR:P18392 , String:511145.b1609 , Swiss-Model:P18392 , UniProt:P18392

Relationship Links: InterPro:IN-FAMILY:IPR003594 , InterPro:IN-FAMILY:IPR003660 , InterPro:IN-FAMILY:IPR003661 , InterPro:IN-FAMILY:IPR004358 , InterPro:IN-FAMILY:IPR005467 , InterPro:IN-FAMILY:IPR009082 , Pfam:IN-FAMILY:PF00512 , Pfam:IN-FAMILY:PF00672 , Pfam:IN-FAMILY:PF02518 , Prints:IN-FAMILY:PR00344 , Prosite:IN-FAMILY:PS50109 , Prosite:IN-FAMILY:PS50885 , Smart:IN-FAMILY:SM00304 , Smart:IN-FAMILY:SM00387 , Smart:IN-FAMILY:SM00388

In Paralogous Gene Group: 122 (29 members)

Reactions known to consume the compound:

RstBA Two-Component Signal Transduction System :
RstB[inner membrane] + ATP → RstB sensory histidine kinase - phosphorylated[inner membrane] + ADP

Reactions known to produce the compound:

RstBA Two-Component Signal Transduction System :
RstA + RstB sensory histidine kinase - phosphorylated[inner membrane] → RstA-Pasp52 + RstB[inner membrane]

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Yamamoto05]
GO:0018106 - peptidyl-histidine phosphorylation Inferred from experiment [Yamamoto05]
GO:0046777 - protein autophosphorylation Inferred from experiment [Yamamoto05]
GO:0007165 - signal transduction Inferred by computational analysis [GOA01]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0023014 - signal transduction by phosphorylation Inferred by computational analysis [GOA01]
Molecular Function: GO:0000155 - phosphorelay sensor kinase activity Inferred by computational analysis [GOA01, Mizuno97]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0004673 - protein histidine kinase activity Inferred by computational analysis [GOA01a]
GO:0004871 - signal transducer activity Inferred by computational analysis [GOA01]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Mizuno97]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01, Mizuno97]

MultiFun Terms: cell structure membrane
information transfer protein related posttranslational modification
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for rstB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 24-Nov-2010 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment State
Transmembrane-Region 4 -> 24
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
 
Transmembrane-Region 136 -> 156
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
 
Conserved-Region 158 -> 210
[UniProt09]
UniProt: HAMP;
 
Conserved-Region 218 -> 425
[UniProt09]
UniProt: Histidine kinase;
 
Phosphorylation-Modification 276
[UniProt13]
UniProt: Phosphohistidine; by autocatalysis; Non-Experimental Qualifier: by similarity.
Unmodified


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b1609 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11233; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Minagawa03: Minagawa S, Ogasawara H, Kato A, Yamamoto K, Eguchi Y, Oshima T, Mori H, Ishihama A, Utsumi R (2003). "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." J Bacteriol 185(13);3696-702. PMID: 12813061

Mizuno97: Mizuno T (1997). "Compilation of all genes encoding two-component phosphotransfer signal transducers in the genome of Escherichia coli." DNA Res 4(2);161-8. PMID: 9205844

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Gene Regulation

Monsieurs05: Monsieurs P, De Keersmaecker S, Navarre WW, Bader MW, De Smet F, McClelland M, Fang FC, De Moor B, Vanderleyden J, Marchal K (2005). "Comparison of the PhoPQ regulon in Escherichia coli and Salmonella typhimurium." J Mol Evol 60(4);462-74. PMID: 15883881

Ogasawara07a: Ogasawara H, Hasegawa A, Kanda E, Miki T, Yamamoto K, Ishihama A (2007). "Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade." J Bacteriol 189(13);4791-9. PMID: 17468243


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc12.