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Escherichia coli K-12 substr. MG1655 Polypeptide: RcsC sensory histidine kinase



Gene: rcsC Accession Numbers: EG10822 (EcoCyc), b2218, ECK2211

Regulation Summary Diagram: ?

Alternative forms of RcsC sensory histidine kinase:
RcsC sensory histidine kinase - asp875 phosphorylated (summary available)
RcsC sensory histidine kinase - his 479 phosphorylated (summary available)

Summary:
RcsC is the sensory histidine kinase of the Rcs phosphorelay system best characterised for its role in the transcriptional regulation of the genes for capsular polysaccharide [Brill88]. RscC is an inner membrane hybrid kinase consisting of an N-terminal periplasmic domain and a C-terminal cytoplasmic region containing H1 and D1 domains [Stout90]. RcsC forms part of a complex phosphorelay in which phosphate travels from the H1 domain in RcsC to the D1 domain of RcsC to the conserved histidine of RcsD and finally to the conserved aspartate of the response regulator RcsB [Takeda01]. The conserved residues his(463) and asp(859) are essential for activation of RcsC [Clarke02]. The precise environmental signal for RscC activation is not known however the sensor responds to growth on a solid surface [Ferrieres03], to osmotic shock [Sledjeski96] and to membrane perturbations [Ebel97]. RscC signalling is required for normal biofilm formation in E. coli [Ferrieres03].

A DNA replication arrest site, TerF is located within the coding sequence of rcsC [Sharma92a].

Reviews: [Clarke10]

Citations: [Mouslim03, Huang06a, Majdalani05, Rogov06, Huang09b]

Gene Citations: [Mukhopadhyay97]

Locations: inner membrane

Map Position: [2,315,049 <- 2,317,850] (49.9 centisomes)
Length: 2802 bp / 933 aa

Molecular Weight of Polypeptide: 104.59 kD (from nucleotide sequence)

pI: 5.94

Unification Links: ASAP:ABE-0007336 , CGSC:17974 , DIP:DIP-10647N , EchoBASE:EB0815 , EcoGene:EG10822 , EcoliWiki:b2218 , Mint:MINT-1267658 , ModBase:P14376 , OU-Microarray:b2218 , PortEco:rcsC , PR:PRO_000023691 , Pride:P14376 , Protein Model Portal:P14376 , RegulonDB:EG10822 , SMR:P14376 , String:511145.b2218 , UniProt:P14376

Relationship Links: InterPro:IN-FAMILY:IPR000014 , InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR003594 , InterPro:IN-FAMILY:IPR003661 , InterPro:IN-FAMILY:IPR004358 , InterPro:IN-FAMILY:IPR005467 , InterPro:IN-FAMILY:IPR009082 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR019017 , PDB:Structure:2AYX , PDB:Structure:2AYY , PDB:Structure:2AYZ , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF00512 , Pfam:IN-FAMILY:PF02518 , Pfam:IN-FAMILY:PF09456 , Prints:IN-FAMILY:PR00344 , Prosite:IN-FAMILY:PS50109 , Prosite:IN-FAMILY:PS50110 , Prosite:IN-FAMILY:PS50112 , Prosite:IN-FAMILY:PS51426 , Smart:IN-FAMILY:SM00091 , Smart:IN-FAMILY:SM00387 , Smart:IN-FAMILY:SM00388 , Smart:IN-FAMILY:SM00448

In Paralogous Gene Group: 121 (40 members) , 122 (29 members)

Reactions known to consume the compound:

RcsCDB Two-Component Signal Transduction System :
ATP + RcsC[inner membrane] → ADP + RcsC-Phis[inner membrane]

Reactions known to produce the compound:

RcsCDB Two-Component Signal Transduction System :
RcsD[inner membrane] + RcsC-Pasp[inner membrane] → RcsD-Phis[inner membrane] + RcsC[inner membrane]

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment [Takeda01]
GO:0044010 - single-species biofilm formation Inferred from experiment [Ferrieres03]
GO:0046777 - protein autophosphorylation Inferred from experiment [Takeda01]
GO:0071470 - cellular response to osmotic stress Inferred from experiment [Sledjeski96]
Molecular Function: GO:0000155 - phosphorelay sensor kinase activity Inferred from experiment [Takeda01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [DiazMejia09, Zhang07, Daley05, Stout90]
GO:0016021 - integral component of membrane Inferred by computational analysis [Stout90]

MultiFun Terms: cell structure capsule (M and K antigens)
cell structure membrane
cell structure surface antigens (ECA, O antigen of LPS)
information transfer protein related posttranslational modification
metabolism biosynthesis of macromolecules (cellular constituents) colanic acid (M antigen)
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for rcsC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment State
Extrinsic-Sequence-Variant 315
[UniProt10a]
Alternate sequence: F → V; UniProt: (in strain: O9:K30:H12);
 
Phosphorylation-Modification 479
[UniProt13]
UniProt: Phosphohistidine; by autocatalysis; Non-Experimental Qualifier: by similarity.
Unmodified
Extrinsic-Sequence-Variant 776
[UniProt10a]
Alternate sequence: P → E; UniProt: (in strain: O9:K30:H12);
 
4-aspartylphosphate-Modification 875
[UniProt11a]
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
Unmodified
Extrinsic-Sequence-Variant 890
[UniProt10a]
Alternate sequence: A → T; UniProt: (in strain: O9:K30:H12);
 


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2218 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10822; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brill88: Brill JA, Quinlan-Walshe C, Gottesman S (1988). "Fine-structure mapping and identification of two regulators of capsule synthesis in Escherichia coli K-12." J Bacteriol 1988;170(6);2599-611. PMID: 2836365

Clarke02: Clarke DJ, Joyce SA, Toutain CM, Jacq A, Holland IB (2002). "Genetic analysis of the RcsC sensor kinase from Escherichia coli K-12." J Bacteriol 184(4);1204-8. PMID: 11807084

Clarke10: Clarke DJ (2010). "The Rcs phosphorelay: more than just a two-component pathway." Future Microbiol 5(8);1173-84. PMID: 20722597

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Ebel97: Ebel W, Vaughn GJ, Peters HK, Trempy JE (1997). "Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli." J Bacteriol 179(21);6858-61. PMID: 9352941

Ferrieres03: Ferrieres L, Clarke DJ (2003). "The RcsC sensor kinase is required for normal biofilm formation in Escherichia coli K-12 and controls the expression of a regulon in response to growth on a solid surface." Mol Microbiol 50(5);1665-82. PMID: 14651646

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Huang06a: Huang YH, Ferrieres L, Clarke DJ (2006). "The role of the Rcs phosphorelay in Enterobacteriaceae." Res Microbiol 157(3);206-12. PMID: 16427772

Huang09b: Huang YH, Ferrieres L, Clarke DJ (2009). "Comparative functional analysis of the RcsC sensor kinase from different Enterobacteriaceae." FEMS Microbiol Lett 293(2);248-54. PMID: 19260968

Majdalani05: Majdalani N, Gottesman S (2005). "The Rcs phosphorelay: a complex signal transduction system." Annu Rev Microbiol 59;379-405. PMID: 16153174

Mouslim03: Mouslim C, Latifi T, Groisman EA (2003). "Signal-dependent requirement for the co-activator protein RcsA in transcription of the RcsB-regulated ugd gene." J Biol Chem 278(50);50588-95. PMID: 14514676

Mukhopadhyay97: Mukhopadhyay S, Schellhorn HE (1997). "Identification and characterization of hydrogen peroxide-sensitive mutants of Escherichia coli: genes that require OxyR for expression." J Bacteriol 1997;179(2);330-8. PMID: 8990283

Rogov06: Rogov VV, Rogova NY, Bernhard F, Koglin A, Lohr F, Dotsch V (2006). "A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains." J Mol Biol 364(1);68-79. PMID: 17005198

Sharma92a: Sharma B, Hill TM (1992). "TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene." J Bacteriol 174(23);7854-8. PMID: 1447156

Sledjeski96: Sledjeski DD, Gottesman S (1996). "Osmotic shock induction of capsule synthesis in Escherichia coli K-12." J Bacteriol 178(4);1204-6. PMID: 8576059

Stout90: Stout V, Gottesman S (1990). "RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli." J Bacteriol 1990;172(2);659-69. PMID: 2404948

Takeda01: Takeda S, Fujisawa Y, Matsubara M, Aiba H, Mizuno T (2001). "A novel feature of the multistep phosphorelay in Escherichia coli: a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour." Mol Microbiol 40(2);440-50. PMID: 11309126

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc14.