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Escherichia coli K-12 substr. MG1655 Enzyme: nicotinamidase / pyrazinamidase



Gene: pncA Accession Numbers: EG11135 (EcoCyc), b1768, ECK1766

Synonyms: nam, ydjB

Regulation Summary Diagram: ?

Summary:
pncA in E. coli K-12 encodes a nicotinamidase that is active in the cyclical salvage pathway for production of NAD from nicotinamide [Pardee71].

PncA: "pyridine nucleotide cycle" [Tritz70]

Gene Citations: [Jerlstrom89]

Locations: cytosol

Map Position: [1,849,911 -> 1,850,552] (39.87 centisomes)
Length: 642 bp / 213 aa

Molecular Weight of Polypeptide: 23.362 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005891 , DIP:DIP-10520N , EchoBASE:EB1125 , EcoGene:EG11135 , EcoliWiki:b1768 , ModBase:P21369 , OU-Microarray:b1768 , PortEco:pncA , PR:PRO_000023560 , Pride:P21369 , Protein Model Portal:P21369 , RefSeq:NP_416282 , RegulonDB:EG11135 , SMR:P21369 , String:511145.b1768 , UniProt:P21369

Relationship Links: InterPro:IN-FAMILY:IPR000868 , Pfam:IN-FAMILY:PF00857

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
GO:0019363 - pyridine nucleotide biosynthetic process Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0008936 - nicotinamidase activity Inferred from experiment Inferred by computational analysis [GOA01a, Pardee71]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism biosynthesis of building blocks cofactors, small molecule carriers nicotinamide adenine dinucleotide

Essentiality data for pncA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: nicotinamidase

Synonyms: nicotinamide amidohydrolase, NAm amidohydrolase, NAm deamidase, nicotinamide deamidase

EC Number: 3.5.1.19

nicotinamide + H2O <=> ammonium + nicotinate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: NAD salvage pathway I


Enzymatic reaction of: pyrazinamidase

Synonyms: PZAase

EC Number: 3.5.1.-

pyrazinamide + H2O <=> ammonium + pyrazine-2-carboxylate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.

Summary:
The pncA-encoded nicotinamidase also has pyrazinamidase activity. Pyrazinamide is an antibiotic with a structure similar to nicotinamide [Frothingham96]


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
7/20/1999 (pkarp) Merged genes G188/pncA and EG11135/b1768
10/20/97 Gene b1768 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11135; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Frothingham96: Frothingham R, Meeker-O'Connell WA, Talbot EA, George JW, Kreuzer KN (1996). "Identification, cloning, and expression of the Escherichia coli pyrazinamidase and nicotinamidase gene, pncA." Antimicrob Agents Chemother 1996;40(6);1426-31. PMID: 8726014

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Jerlstrom89: Jerlstrom PG, Bezjak DA, Jennings MP, Beacham IR (1989). "Structure and expression in Escherichia coli K-12 of the L-asparaginase I-encoding ansA gene and its flanking regions." Gene 1989;78(1);37-46. PMID: 2670682

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pardee71: Pardee AB, Benz EJ, St Peter DA, Krieger JN, Meuth M, Trieshmann HW (1971). "Hyperproduction and purification of nicotinamide deamidase, a microconstitutive enzyme of Escherichia coli." J Biol Chem 1971;246(22);6792-6. PMID: 4399474

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Tritz70: Tritz GJ, Matney TS, Gholson RK (1970). "Mapping of the nadB locus adjacent to a previously undescribed purine locus in Escherichia coli K-12." J Bacteriol 102(2);377-81. PMID: 4315893

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc13.