Escherichia coli K-12 substr. MG1655 Polypeptide: HcaT MFS transporter

Gene: hcaT Accession Numbers: G7330 (EcoCyc), b2536, ECK2533

Synonyms: yfhS

Regulation Summary Diagram: ?

Regulation summary diagram for hcaT

HcaT is a member of the major facilitator superfamily (MFS) of transporters [Pao98]. HcaT is a putative 3-phenylpropionate transporter. The hcaT gene is located immediately downstream of the hcaR gene, whose product regulates expression of the hcaA-D operon responsible for catabolism of 3-phenylpropionic acid [Diaz98]. Membrane topology predictions using experimentally determined C terminus locations indicate that HcaT has 12 transmembrane helices and the C-terminus is located in the cytoplasm [Rapp04].

Citations: [Riley06]

Locations: inner membrane

Map Position: [2,664,729 <- 2,665,868] (57.43 centisomes, 207°)
Length: 1140 bp / 379 aa

Molecular Weight of Polypeptide: 41.593 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008348 , EchoBASE:EB3227 , EcoGene:EG13454 , EcoliWiki:b2536 , ModBase:Q47142 , OU-Microarray:b2536 , PortEco:hcaT , Protein Model Portal:Q47142 , RefSeq:NP_417031 , RegulonDB:G7330 , SMR:Q47142 , String:511145.b2536 , UniProt:Q47142

Relationship Links: InterPro:IN-FAMILY:IPR000576 , InterPro:IN-FAMILY:IPR005276 , InterPro:IN-FAMILY:IPR020846 , InterPro:IN-FAMILY:IPR026032 , Pfam:IN-FAMILY:PF01306

In Paralogous Gene Group: 232 (3 members)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0055085 - transmembrane transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0015293 - symporter activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Rapp04]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for hcaT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Sequence Features

Protein sequence of HcaT MFS transporter with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 5 -> 31
UniProt: Helical; Name=1;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 38 -> 66
UniProt: Helical; Name=2;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 71 -> 96
UniProt: Helical; Name=3;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 101 -> 118
UniProt: Helical; Name=4;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 130 -> 152
UniProt: Helical; Name=5;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 156 -> 175
UniProt: Helical; Name=6;; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 191
[Diaz98, UniProt10]
UniProt: (in Ref. 2; CAA71954);
Transmembrane-Region 208 -> 227
UniProt: Helical; Name=7;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 232 -> 256
UniProt: Helical; Name=8;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 267 -> 286
UniProt: Helical; Name=9;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 290 -> 312
UniProt: Helical; Name=10;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 324 -> 351
UniProt: Helical; Name=11;; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 355 -> 375
UniProt: Helical; Name=12;; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

Diaz98: Diaz E, Ferrandez A, Garcia JL (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." J Bacteriol 1998;180(11);2915-23. PMID: 9603882

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

Rapp04: Rapp M, Drew D, Daley DO, Nilsson J, Carvalho T, Melen K, De Gier JW, Von Heijne G (2004). "Experimentally based topology models for E. coli inner membrane proteins." Protein Sci 13(4);937-45. PMID: 15044727

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri May 29, 2015, biocyc12.