Escherichia coli K-12 substr. MG1655 Polypeptide: predicted pilin chaperone

Gene: yraI Accession Numbers: G7638 (EcoCyc), b3143, ECK3131

Regulation Summary Diagram: ?

Regulation summary diagram for yraI

yraHIJK is a putative chaperone-usher fimbrial operon in E.coli K-12 [Zhai02, Korea10]. The operon is cryptic under normal laboratory conditions but when constitutive expression is induced in a strain that lacks the type 1 fimbrial complex the yra operon promotes adhesion to eukaryotic epithelial cells (T4 bladder cells) [Korea10]. Expression of the yra operon is regulated by the global regulators H-NS and CRP cAMP [Korea10].

Citations: [Korea11, ParadisBleau14]

Locations: periplasmic space

Map Position: [3,286,112 -> 3,286,807] (70.83 centisomes, 255°)
Length: 696 bp / 231 aa

Molecular Weight of Polypeptide: 25.676 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010331 , EchoBASE:EB2627 , EcoGene:EG12774 , EcoliWiki:b3143 , ModBase:P42914 , OU-Microarray:b3143 , PortEco:yraI , Protein Model Portal:P42914 , RefSeq:NP_417612 , RegulonDB:G7638 , SMR:P42914 , String:511145.b3143 , Swiss-Model:P42914 , UniProt:P42914

Relationship Links: InterPro:IN-FAMILY:IPR001829 , InterPro:IN-FAMILY:IPR008962 , InterPro:IN-FAMILY:IPR016147 , InterPro:IN-FAMILY:IPR016148 , InterPro:IN-FAMILY:IPR018046 , Pfam:IN-FAMILY:PF00345 , Pfam:IN-FAMILY:PF02753 , Prints:IN-FAMILY:PR00969 , Prosite:IN-FAMILY:PS00635

In Paralogous Gene Group: 151 (8 members)

GO Terms:

Biological Process: GO:0006457 - protein folding Inferred by computational analysis [Korea10]
GO:0043711 - pilus organization Inferred by computational analysis [GOA01, Gaudet10, Korea10]
GO:0061077 - chaperone-mediated protein folding Inferred by computational analysis [GOA01, Gaudet10]
GO:0071555 - cell wall organization Inferred by computational analysis [GOA01]
Molecular Function: GO:0044183 - protein binding involved in protein folding Inferred by computational analysis [Gaudet10]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [GOA01, DiazMejia09]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: information transfer protein related chaperoning, repair (refolding)

Essentiality data for yraI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 13-Apr-2010 by Mackie A , Macquarie University

Sequence Features

Protein sequence of predicted pilin chaperone with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 23
Chain 25 -> 231
UniProt: Probable fimbrial chaperone YraI.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Korea10: Korea CG, Badouraly R, Prevost MC, Ghigo JM, Beloin C (2010). "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities." Environ Microbiol 12(7):1957-77. PMID: 20345943

Korea11: Korea CG, Ghigo JM, Beloin C (2011). "The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile arsenal of sugar-binding lectins potentially involved in surface-colonisation and tissue tropism." Bioessays 33(4);300-11. PMID: 21344461

ParadisBleau14: Paradis-Bleau C, Kritikos G, Orlova K, Typas A, Bernhardt TG (2014). "A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism." PLoS Genet 10(1);e1004056. PMID: 24391520

UniProt14a: UniProt Consortium (2014). "UniProt version 2014-08 released on 2014-08-01 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Zhai02: Zhai Y, Saier MH (2002). "The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes." Protein Sci 11(9);2196-207. PMID: 12192075

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, BIOCYC11A.