|Gene:||yqjA||Accession Numbers: G7609 (EcoCyc), b3095, ECK3086|
A yghB yqjA double null strain has a synthetic growth defect at elevated temperatures, forms chains of cells at permissive temperatures, and can be complemented by yghB, yqjA, yohD, or yabI expressed from a plasmid [Thompkins08]. The double and single mutants displayed altered phospholipid compositions [Thompkins08].
An E. coli ΔyqjA ΔyghB strain is hypersensitive to a number of structurally diverse drugs and dyes including ethidium bromide, methyl viologen, acriflavine, nalidixic acid and β-lactam antibiotics. A wild-type response is restored by expression of yqjA from a plasmid [Kumar13]. Mutation of the membrane embedded acidic amino acids E39 (glutamic acid) or D51 (aspartic acid) in plasmid-expressed YqjA compromises its ability to restore wild-type resistance [Kumar13]. Overexpression of yqjA in a wild-type background does not result in increased resistance to any of the compounds tested. Overexpression of the mdfA antiporter restores the wild-type resistance of an E. coli ΔyqjA ΔyghB strain to all componds tested as does growth in the presence of 400mM NaCl or with increased amounts of cations or at pH 6 [Kumar13, Thompkins08]. Deletion of yqjA and yghB may indirectly cause drug sensitivity due to impairment of the proton-motive force (PMF) [Kumar13].
YqjA is a member of the DedA family of inner membrane proteins [Thompkins08, Doerrler13]. E. coli K-12 contains 7 other DedA family members, namely DedA, YghB, YabI, YohD, YqaA, YdjX and YdjZ. Collectively, the DedA family is essential in E. coli K-12 [Boughner12].
Transcription of yqjA is regulated by RpoE (σE [Dartigalongue01].
ecfL: extracytoplasmic function [Dartigalongue01].
Locations: inner membrane
|Map Position: [3,245,795 -> 3,246,457] (69.96 centisomes, 252°)||Length: 663 bp / 220 aa|
Molecular Weight of Polypeptide: 24.585 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0010181 , EchoBASE:EB2596 , EcoGene:EG12740 , EcoliWiki:b3095 , OU-Microarray:b3095 , PortEco:yqjA , Protein Model Portal:P0AA63 , RefSeq:NP_417566 , RegulonDB:G7609 , String:511145.b3095 , UniProt:P0AA63
In Paralogous Gene Group: 343 (8 members)
|Biological Process:||GO:0042493 - response to drug
GO:0043093 - FtsZ-dependent cytokinesis [Thompkins08]
GO:0055085 - transmembrane transport [Kumar13]
|Molecular Function:||GO:0022857 - transmembrane transporter activity [Kumar13]|
|Cellular Component:||GO:0005886 - plasma membrane
[UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane [Thompkins08]
GO:0016020 - membrane [UniProtGOA11a]
GO:0016021 - integral component of membrane [UniProtGOA11a]
|MultiFun Terms:||cell structure → membrane|
|metabolism → energy production/transport|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 1]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 2]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 1]|
|Transmembrane-Region||28 -> 48|
|Transmembrane-Region||53 -> 73|
|Transmembrane-Region||74 -> 94|
|Transmembrane-Region||155 -> 175|
|Transmembrane-Region||192 -> 212|
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Kumar13: Kumar S, Doerrler WT (2013). "Members of the conserved DedA family are likely membrane transporters and are required for drug resistance in E. coli." Antimicrob Agents Chemother. PMID: 24277026
Thompkins08: Thompkins K, Chattopadhyay B, Xiao Y, Henk MC, Doerrler WT (2008). "Temperature sensitivity and cell division defects in an Escherichia coli strain with mutations in yghB and yqjA, encoding related and conserved inner membrane proteins." J Bacteriol 190(13);4489-500. PMID: 18456815
Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175
©2014 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493