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Escherichia coli K-12 substr. MG1655 Polypeptide: membrane transport protein; required for proton-motive force (PMF) dependent drug efflux



Gene: yqjA Accession Numbers: G7609 (EcoCyc), b3095, ECK3086

Synonyms: ecfL

Regulation Summary Diagram: ?

Summary:
A yghB yqjA double null strain has a synthetic growth defect at elevated temperatures, forms chains of cells at permissive temperatures, and can be complemented by yghB, yqjA, yohD, or yabI expressed from a plasmid [Thompkins08]. The double and single mutants displayed altered phospholipid compositions [Thompkins08].

An E. coli ΔyqjA ΔyghB strain is hypersensitive to a number of structurally diverse drugs and dyes including ethidium bromide, methyl viologen, acriflavine, nalidixic acid and β-lactam antibiotics. A wild-type response is restored by expression of yqjA from a plasmid [Kumar13]. Mutation of the membrane embedded acidic amino acids E39 (glutamic acid) or D51 (aspartic acid) in plasmid-expressed YqjA compromises its ability to restore wild-type resistance [Kumar13]. Overexpression of yqjA in a wild-type background does not result in increased resistance to any of the compounds tested. Overexpression of the mdfA antiporter restores the wild-type resistance of an E. coli ΔyqjA ΔyghB strain to all componds tested as does growth in the presence of 400mM NaCl or with increased amounts of cations or at pH 6 [Kumar13, Thompkins08]. Deletion of yqjA and yghB may indirectly cause drug sensitivity due to impairment of the proton-motive force (PMF) [Kumar13].

YqjA is a member of the DedA family of inner membrane proteins [Thompkins08, Doerrler13]. E. coli K-12 contains 7 other DedA family members, namely DedA, YghB, YabI, YohD, YqaA, YdjX and YdjZ. Collectively, the DedA family is essential in E. coli K-12 [Boughner12].

Transcription of yqjA is regulated by RpoE (σE [Dartigalongue01].

ecfL: extracytoplasmic function [Dartigalongue01].

Locations: inner membrane

Map Position: [3,245,795 -> 3,246,457] (69.96 centisomes)
Length: 663 bp / 220 aa

Molecular Weight of Polypeptide: 24.585 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010181 , EchoBASE:EB2596 , EcoGene:EG12740 , EcoliWiki:b3095 , OU-Microarray:b3095 , PortEco:yqjA , Protein Model Portal:P0AA63 , RefSeq:NP_417566 , RegulonDB:G7609 , String:511145.b3095 , UniProt:P0AA63

Relationship Links: InterPro:IN-FAMILY:IPR015414 , Pfam:IN-FAMILY:PF09335

In Paralogous Gene Group: 343 (8 members)

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0042493 - response to drug Inferred from experiment [Kumar13]
GO:0043093 - FtsZ-dependent cytokinesis Inferred from experiment [Thompkins08]
GO:0055085 - transmembrane transport Inferred from experiment [Kumar13]
Molecular Function: GO:0022857 - transmembrane transporter activity Inferred from experiment [Kumar13]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Thompkins08]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
metabolism energy production/transport

Essentiality data for yqjA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Curated 06-Sep-2006 by Keseler I , SRI International
Last-Curated ? 11-Aug-2008 by Johnson A , JCVI


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 28 -> 48
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 53 -> 73
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 74 -> 94
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 155 -> 175
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 192 -> 212
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Boughner12: Boughner LA, Doerrler WT (2012). "Multiple deletions reveal the essentiality of the DedA membrane protein family in Escherichia coli." Microbiology 158(Pt 5);1162-71. PMID: 22301910

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

Dartigalongue01: Dartigalongue C, Missiakas D, Raina S (2001). "Characterization of the Escherichia coli sigma E regulon." J Biol Chem 276(24);20866-75. PMID: 11274153

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Doerrler13: Doerrler WT, Sikdar R, Kumar S, Boughner LA (2013). "New functions for the ancient DedA membrane protein family." J Bacteriol 195(1);3-11. PMID: 23086209

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kumar13: Kumar S, Doerrler WT (2013). "Members of the conserved DedA family are likely membrane transporters and are required for drug resistance in E. coli." Antimicrob Agents Chemother. PMID: 24277026

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Thompkins08: Thompkins K, Chattopadhyay B, Xiao Y, Henk MC, Doerrler WT (2008). "Temperature sensitivity and cell division defects in an Escherichia coli strain with mutations in yghB and yqjA, encoding related and conserved inner membrane proteins." J Bacteriol 190(13);4489-500. PMID: 18456815

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175

Yamamoto06: Yamamoto K, Ishihama A (2006). "Characterization of copper-inducible promoters regulated by CpxA/CpxR in Escherichia coli." Biosci Biotechnol Biochem 70(7);1688-95. PMID: 16861804


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.