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Escherichia coli K-12 substr. MG1655 Polypeptide: putative tRNA synthetase



Gene: ygjH Accession Numbers: G7597 (EcoCyc), b3074, ECK3064

Regulation Summary Diagram: ?

Summary:
No information about this protein was found by a literature search conducted on January 5, 2006.

Locations: cytosol

Map Position: [3,218,937 <- 3,219,269] (69.38 centisomes)
Length: 333 bp / 110 aa

Molecular Weight of Polypeptide: 12.315 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010094 , DIP:DIP-12234N , EchoBASE:EB2578 , EcoGene:EG12719 , EcoliWiki:b3074 , Mint:MINT-1283890 , ModBase:P42589 , OU-Microarray:b3074 , PortEco:ygjH , Protein Model Portal:P42589 , RefSeq:NP_417545 , RegulonDB:G7597 , SMR:P42589 , String:511145.b3074 , UniProt:P42589

Relationship Links: InterPro:IN-FAMILY:IPR002547 , InterPro:IN-FAMILY:IPR012340 , PDB:Structure:3ERS , Pfam:IN-FAMILY:PF01588 , Prosite:IN-FAMILY:PS50886

In Paralogous Gene Group: 378 (2 members)

GO Terms:

Molecular Function: GO:0000049 - tRNA binding Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs Conserved-ORFs

Essentiality data for ygjH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Conserved-Region 8 -> 110
[UniProt09]
UniProt: tRNA-binding;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc13.