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discounted EARLY registration ends Dec 31, 2014
BioCyc websites MAYBE down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites MAYBE down
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for maintenance.
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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted peptidase



Gene: ygeY Accession Numbers: G7491 (EcoCyc), b2872, ECK2868

Regulation Summary Diagram: ?

Summary:
No information about this protein was found by a literature search conducted on November 15, 2013.

Citations: [ParadisBleau14]

Locations: cytosol

Map Position: [3,006,786 -> 3,007,997] (64.81 centisomes)
Length: 1212 bp / 403 aa

Molecular Weight of Polypeptide: 44.804 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009434 , EchoBASE:EB2867 , EcoGene:EG13055 , EcoliWiki:b2872 , ModBase:P65807 , OU-Microarray:b2872 , PortEco:ygeY , Pride:P65807 , Protein Model Portal:P65807 , RefSeq:NP_417348 , RegulonDB:G7491 , SMR:P65807 , String:511145.b2872 , UniProt:P65807

Relationship Links: InterPro:IN-FAMILY:IPR001261 , InterPro:IN-FAMILY:IPR002933 , InterPro:IN-FAMILY:IPR011650 , InterPro:IN-FAMILY:IPR017706 , Pfam:IN-FAMILY:PF01546 , Pfam:IN-FAMILY:PF07687 , Prosite:IN-FAMILY:PS00758

In Paralogous Gene Group: 464 (3 members)

GO Terms:

Biological Process: GO:0006508 - proteolysis Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0008233 - peptidase activity Inferred by computational analysis [UniProtGOA11a]
GO:0008237 - metallopeptidase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

Gene Class: ORFs

Essentiality data for ygeY knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 15-Nov-2013 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Metal-Binding-Site 81
[UniProt10a]
UniProt: Cobalt or zinc 1; Non-Experimental Qualifier: by similarity;
Active-Site 83
[UniProt10a]
UniProt: Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 114
[UniProt10a]
UniProt: Cobalt or zinc 1; Non-Experimental Qualifier: by similarity;
Active-Site 148
[UniProt10a]
UniProt: Proton acceptor; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 149
[UniProt10a]
UniProt: Cobalt or zinc 2; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 174
[UniProt10a]
UniProt: Cobalt or zinc 1; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 374
[UniProt10a]
UniProt: Cobalt or zinc 2; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

ParadisBleau14: Paradis-Bleau C, Kritikos G, Orlova K, Typas A, Bernhardt TG (2014). "A genome-wide screen for bacterial envelope biogenesis mutants identifies a novel factor involved in cell wall precursor metabolism." PLoS Genet 10(1);e1004056. PMID: 24391520

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 27, 2014, biocyc12.