Escherichia coli K-12 substr. MG1655 Protein: CSD sulfur transfer protein

Gene: csdE Accession Numbers: G7455 (EcoCyc), b2811, ECK2807

Synonyms: ygdK

Regulation Summary Diagram: ?

Regulation summary diagram for csdE

Subunit composition of CSD sulfur transfer protein = [CsdE]2
         CSD sulfur transfer protein = CsdE

CsdA and CsdE combine to form the CSD sulfur transfer system. CsdA activity doubles in the presence of CsdE, which contains a cysteine residue (C61) that acts to accept sulfur liberated via the desulfinase activity of CsdA [Loiseau05]. CsdE also interacts with and may be able to transfer sulfur to CsdL [Trotter09].

An NMR solution structure of CsdE has been solved [Liu05b]. CsdE migrates as a dimer on a gel filtration column [Loiseau05].

CsdE: "cysteine sulfinate desulfinase"

Locations: cytosol

Map Position: [2,942,564 -> 2,943,007] (63.42 centisomes, 228°)
Length: 444 bp / 147 aa

Molecular Weight of Polypeptide: 15.94 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009220 , DIP:DIP-12143N , EchoBASE:EB2892 , EcoGene:EG13083 , EcoliWiki:b2811 , ModBase:P0AGF2 , OU-Microarray:b2811 , PortEco:csdE , Pride:P0AGF2 , Protein Model Portal:P0AGF2 , RefSeq:NP_417291 , RegulonDB:G7455 , SMR:P0AGF2 , String:511145.b2811 , Swiss-Model:P0AGF2 , UniProt:P0AGF2

Relationship Links: InterPro:IN-FAMILY:IPR003808 , InterPro:IN-FAMILY:IPR017763 , PDB:Structure:1NI7 , Pfam:IN-FAMILY:PF02657

In Paralogous Gene Group: 329 (2 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0016226 - iron-sulfur cluster assembly Inferred from experiment [Loiseau05]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14, Kim13b, Loiseau05, Trotter09]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism metabolism of other compounds sulfur metabolism

Essentiality data for csdE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Created 14-Jan-2010 by Keseler I , SRI International
Last-Curated ? 14-Jan-2010 by Keseler I , SRI International

Sequence Features

Protein sequence of CSD sulfur transfer protein with features indicated

Feature Class Location Citations Comment
Active-Site 61
UniProt: Cysteine persulfide intermediate.
Mutagenesis-Variant 61
[Loiseau05, UniProt14]
UniProt: Unable to exert a stimulatory effect on the cysteine desulfurase activity of CsdA and to accept sulfur. Retains the ability to interact with CsdA.
Sulfation-Modification 61
This cysteine accepts sulfur liberated by the enzymatic reaction of CsdE.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kim13b: Kim S, Park S (2013). "Structural changes during cysteine desulfurase CsdA and sulfur acceptor CsdE interactions provide insight into the trans-persulfuration." J Biol Chem 288(38);27172-80. PMID: 23913692

Liu05b: Liu G, Li Z, Chiang Y, Acton T, Montelione GT, Murray D, Szyperski T (2005). "High-quality homology models derived from NMR and X-ray structures of E. coli proteins YgdK and Suf E suggest that all members of the YgdK/Suf E protein family are enhancers of cysteine desulfurases." Protein Sci 14(6);1597-608. PMID: 15930006

Loiseau05: Loiseau L, Ollagnier-de Choudens S, Lascoux D, Forest E, Fontecave M, Barras F (2005). "Analysis of the heteromeric CsdA-CsdE cysteine desulfurase, assisting Fe-S cluster biogenesis in Escherichia coli." J Biol Chem 280(29);26760-9. PMID: 15901727

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

Trotter09: Trotter V, Vinella D, Loiseau L, Ollagnier de Choudens S, Fontecave M, Barras F (2009). "The CsdA cysteine desulphurase promotes Fe/S biogenesis by recruiting Suf components and participates to a new sulphur transfer pathway by recruiting CsdL (ex-YgdL), a ubiquitin-modifying-like protein." Mol Microbiol 74(6);1527-42. PMID: 20054882

UniProt14: UniProt Consortium (2014). "UniProt version 2014-01 released on 2014-01-01 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, BIOCYC14A.