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Escherichia coli K-12 substr. MG1655 Polypeptide: protein induced by aluminum



Gene: ais Accession Numbers: G7165 (EcoCyc), b2252, ECK2245

Regulation Summary Diagram: ?

Summary:
Expression of ais is significantly induced by addition of 0.2 mM ZnSO4 to the medium [Lee05].

Locations: periplasmic space

Map Position: [2,363,040 <- 2,363,642] (50.93 centisomes)
Length: 603 bp / 200 aa

Molecular Weight of Polypeptide: 22.257 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007451 , EchoBASE:EB2949 , EcoGene:EG13155 , EcoliWiki:b2252 , ModBase:P45565 , OU-Microarray:b2252 , PortEco:ais , PR:PRO_000022073 , Pride:P45565 , Protein Model Portal:P45565 , RefSeq:NP_416755 , RegulonDB:G7165 , SMR:P45565 , String:511145.b2252 , UniProt:P45565

Relationship Links: InterPro:IN-FAMILY:IPR011310 , InterPro:IN-FAMILY:IPR013078 , Pfam:IN-FAMILY:PF00300 , Prosite:IN-FAMILY:PS00175

GO Terms:

Biological Process: GO:0008653 - lipopolysaccharide metabolic process Inferred by computational analysis [UniProtGOA12]
GO:0016311 - dephosphorylation Inferred by computational analysis [GOA01a]
Molecular Function: GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016791 - phosphatase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

Gene Class: ORFs Conserved-ORFs

Essentiality data for ais knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 13-Dec-2005 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 25
[UniProt10a]
UniProt: Non-Experimental Qualifier: potential;
Chain 26 -> 200
[UniProt09]
UniProt: Lipopolysaccharide core heptose(II)- phosphate phosphatase;
Sequence-Conflict 200
[Guzzo95, UniProt10]
Alternate sequence: H → Y; UniProt: (in Ref. 1; CAA58754);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Guzzo95: Guzzo A., Macintyre G., Diorio C., Salmon K., Dubow M.S. (1995). "Identification, sequencing and characterization of an aluminium-inducible Escherichia coli gene." Data submission to EMBL/GenBank/DDBJ databases on 1995-01.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lee05: Lee LJ, Barrett JA, Poole RK (2005). "Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc." J Bacteriol 187(3);1124-34. PMID: 15659689

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC13A.