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Escherichia coli K-12 substr. MG1655 Polypeptide: ATP-dependent helicase



Gene: hrpA Accession Numbers: G6732 (EcoCyc), b1413, ECK1406

Regulation Summary Diagram: ?

Summary:
HrpA has sequence similarity to DEAH-box RNA helicases [Moriya95]. HrpA is involved in the post-transcriptional processing of the daa operon mRNA, which encodes proteins involved in fimbrial biogenesis of an enteropathogenic E. coli strain. Activity requires the predicted nucleotide triphosphate binding and hydrolysis functions [Koo04]. The ATPase activity of His-tagged partially purified HrpA has been measured [Jain06].

HrpA is not essential for growth [Moriya95].

HrpA: "DEAH-family RNA helicase-like protein" [Moriya95]

Locations: cytosol

Map Position: [1,481,142 -> 1,484,987] (31.92 centisomes)
Length: 3846 bp / 1281 aa

Molecular Weight of Polypeptide: 146.79 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004718 , DIP:DIP-9937N , EchoBASE:EB2772 , EcoGene:EG12936 , EcoliWiki:b1413 , Mint:MINT-1229564 , ModBase:P43329 , OU-Microarray:b1413 , PortEco:hrpA , PR:PRO_000022922 , Pride:P43329 , Protein Model Portal:P43329 , RefSeq:NP_415931 , RegulonDB:G6732 , SMR:P43329 , String:511145.b1413 , UniProt:P43329

Relationship Links: InterPro:IN-FAMILY:IPR001650 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR007502 , InterPro:IN-FAMILY:IPR010222 , InterPro:IN-FAMILY:IPR011545 , InterPro:IN-FAMILY:IPR011709 , InterPro:IN-FAMILY:IPR014001 , InterPro:IN-FAMILY:IPR024590 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00270 , Pfam:IN-FAMILY:PF00271 , Pfam:IN-FAMILY:PF04408 , Pfam:IN-FAMILY:PF07717 , Pfam:IN-FAMILY:PF11898 , Prosite:IN-FAMILY:PS00690 , Prosite:IN-FAMILY:PS51192 , Prosite:IN-FAMILY:PS51194 , Smart:IN-FAMILY:SM00382 , Smart:IN-FAMILY:SM00487 , Smart:IN-FAMILY:SM00490 , Smart:IN-FAMILY:SM00847

In Paralogous Gene Group: 49 (7 members)

GO Terms:

Biological Process: GO:0006397 - mRNA processing Inferred from experiment [Koo04]
GO:0009451 - RNA modification Inferred from experiment [Koo04]
GO:0006200 - ATP catabolic process Inferred by computational analysis [Moriya95, GOA et al., 2001]
Molecular Function: GO:0004386 - helicase activity Inferred from experiment Inferred by computational analysis [UniProt-GOA, 2011, GOA et al., 2001, Koo04]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProt-GOA, 2011]
GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA et al., 2001]
GO:0004004 - ATP-dependent RNA helicase activity Inferred by computational analysis [Moriya95]
GO:0005524 - ATP binding Inferred by computational analysis [UniProt-GOA, 2011, GOA et al., 2001]
GO:0008026 - ATP-dependent helicase activity Inferred by computational analysis [GOA et al., 2001]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProt-GOA, 2011]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol

MultiFun Terms: information transfer RNA related RNA modification

Essentiality data for hrpA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes et al., 2003, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba et al., 2006, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce et al., 2006, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba et al., 2006, Comment 2]

Credits:
Last-Curated ? 14-May-2007 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 152
[Moriya95, UniProt, 2010]
Alternate sequence: K → V; UniProt: (in Ref. 1; BAA07685);
Sequence-Conflict 202
[Moriya95, UniProt, 2010]
Alternate sequence: P → H; UniProt: (in Ref. 1; BAA07685);
Sequence-Conflict 301
[Moriya95, UniProt, 2010]
Alternate sequence: I → missing; UniProt: (in Ref. 1; BAA07685);
Sequence-Conflict 304
[Moriya95, UniProt, 2010]
Alternate sequence: L → P; UniProt: (in Ref. 1; BAA07685);
Sequence-Conflict 705
[Moriya95, UniProt, 2010]
Alternate sequence: V → T; UniProt: (in Ref. 1; BAA07685);
Sequence-Conflict 869
[Moriya95, UniProt, 2010]
Alternate sequence: E → P; UniProt: (in Ref. 1; BAA07685);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba et al., 2006: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes et al., 2003: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA et al., 2001: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jain06: Jain C (2006). "Overexpression and purification of tagged Escherichia coli proteins using a chromosomal knock-in strategy." Protein Expr Purif 46(2);294-8. PMID: 16139517

Joyce et al., 2006: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Koo04: Koo JT, Choe J, Moseley SL (2004). "HrpA, a DEAH-box RNA helicase, is involved in mRNA processing of a fimbrial operon in Escherichia coli." Mol Microbiol 52(6);1813-26. PMID: 15186427

Moriya95: Moriya H, Kasai H, Isono K (1995). "Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae." Nucleic Acids Res 23(4);595-8. PMID: 7899078

UniProt, 2010: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt-GOA, 2011: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, biocyc11.