Escherichia coli K-12 substr. MG1655 Polypeptide: bacteriophage lambda Bor protein homolog

Gene: borD Accession Numbers: G6312 (EcoCyc), b0557, ECK0549

Synonyms: iss, vboR, bor, ybcU

Regulation Summary Diagram: ?

Regulation summary diagram for borD

VboR is, based on sequence analysis, a lipoprotein in Escherichia coli [Brokx04]. Transcription profile analysis has shown that VboR responds in a PhoP/PhoQ two-component system-dependent manner to the availability of external Mg2+ and has been, therefore, characterized as a member of the Mg2+ stimulon [Minagawa03].

Locations: outer membrane, inner membrane

Map Position: [577,823 <- 578,116] (12.45 centisomes, 45°)
Length: 294 bp / 97 aa

Molecular Weight of Polypeptide: 10.447 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001902 , EchoBASE:EB3401 , EcoGene:EG13637 , EcoliWiki:b0557 , OU-Microarray:b0557 , PortEco:borD , Pride:P77330 , Protein Model Portal:P77330 , RefSeq:NP_415089 , RegulonDB:G6312 , String:511145.b0557 , UniProt:P77330

Relationship Links: InterPro:IN-FAMILY:IPR010438 , Pfam:IN-FAMILY:PF06291 , ProDom:IN-FAMILY:PD020173 , Prosite:IN-FAMILY:PS51257

Genetic Regulation Schematic: ?

Genetic regulation schematic for borD

GO Terms:

Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0009279 - cell outer membrane Inferred by computational analysis [GOA01a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: extrachromosomal prophage genes and phage related functions

Essentiality data for borD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of bacteriophage lambda Bor protein homolog with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 16
UniProt: Non-Experimental Qualifier: potential;
Lipid-Binding-Site 17
UniProt: N-palmitoyl cysteine; Non-Experimental Qualifier: potential;
Chain 17 -> 97
UniProt: Lipoprotein bor homolog from lambdoid prophage DLP12;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brokx04: Brokx SJ, Ellison M, Locke T, Bottorff D, Frost L, Weiner JH (2004). "Genome-wide analysis of lipoprotein expression in Escherichia coli MG1655." J Bacteriol 186(10);3254-8. PMID: 15126489

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Minagawa03: Minagawa S, Ogasawara H, Kato A, Yamamoto K, Eguchi Y, Oshima T, Mori H, Ishihama A, Utsumi R (2003). "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." J Bacteriol 185(13);3696-702. PMID: 12813061

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Aug 4, 2015, biocyc14.