Escherichia coli K-12 substr. MG1655 Enzyme: 7-cyano-7-deazaguanine synthase

Gene: queC Accession Numbers: G6245 (EcoCyc), b0444, ECK0438

Synonyms: ybaX

Regulation Summary Diagram: ?

Regulation summary diagram for queC

QueC appears to be the 7-cyano-7-deazaguanine synthase that catalyzes an early step of queuosine biosynthesis, leading from GTP to the formation of preQ0 [Gaur05]. Queuosine is one of the most complex tRNA modifications, occurring at the wobble position of the GUN anticodon in the Asn, Asp, Tyr, and His tRNAs.

queC rescues the queuosine deficiency phenotype of a mutant E. coli B strain [Gaur05].

The biochemical activity of QueC was studied using the orthologous 7-cyano-7-deazaguanine synthase from Bacillus subtilis [McCarty09].

Map Position: [464,076 <- 464,771] (10.0 centisomes, 36°)
Length: 696 bp / 231 aa

Molecular Weight of Polypeptide: 25.514 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001539 , DIP:DIP-11311N , EchoBASE:EB3041 , EcoGene:EG13252 , EcoliWiki:b0444 , Mint:MINT-1259911 , ModBase:P77756 , OU-Microarray:b0444 , PortEco:queC , PR:PRO_000023669 , Pride:P77756 , Protein Model Portal:P77756 , RefSeq:NP_414978 , RegulonDB:G6245 , SMR:P77756 , String:511145.b0444 , UniProt:P77756

Relationship Links: InterPro:IN-FAMILY:IPR014729 , InterPro:IN-FAMILY:IPR018317 , Panther:IN-FAMILY:PTHR11587 , Pfam:IN-FAMILY:PF06508

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0008616 - queuosine biosynthetic process Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA06, Gaur05]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Butland05]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA06]
GO:0008270 - zinc ion binding Inferred by computational analysis [GOA06]
GO:0016874 - ligase activity Inferred by computational analysis [UniProtGOA11]
GO:0016879 - ligase activity, forming carbon-nitrogen bonds Inferred by computational analysis [GOA06]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: information transfer RNA related RNA modification

Essentiality data for queC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 26-Apr-2011 by Keseler I , SRI International

Enzymatic reaction of: 7-cyano-7-deazaguanine synthase

EC Number:

7-carboxy-7-deazaguanine + ammonium + ATP <=> preQ0 + ADP + phosphate + H+ + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: preQ0 biosynthesis

Sequence Features

Protein sequence of 7-cyano-7-deazaguanine synthase with features indicated

Feature Class Location Citations Comment
Nucleotide-Phosphate-Binding-Region 8 -> 18
UniProt: ATP; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 188
UniProt: Zinc; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 197
UniProt: Zinc; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 200
UniProt: Zinc; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 203
UniProt: Zinc; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Gaur05: Gaur R, Varshney U (2005). "Genetic analysis identifies a function for the queC (ybaX) gene product at an initial step in the queuosine biosynthetic pathway in Escherichia coli." J Bacteriol 187(20);6893-901. PMID: 16199558

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

McCarty09: McCarty RM, Somogyi A, Lin G, Jacobsen NE, Bandarian V (2009). "The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps." Biochemistry 48(18);3847-52. PMID: 19354300

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc13.