Escherichia coli K-12 substr. MG1655 Polypeptide: CP4-6 prophage; predicted phage integrase

Gene: intF Accession Numbers: G6152 (EcoCyc), b0281, ECK0280

Synonyms: yagO

Regulation Summary Diagram: ?

Regulation summary diagram for intF

No information about this protein was found by a literature search conducted on January 18, 2007.

Map Position: [294,920 <- 296,320] (6.36 centisomes, 23°)
Length: 1401 bp / 466 aa

Molecular Weight of Polypeptide: 53.074 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000979 , EchoBASE:EB3324 , EcoGene:EG13554 , EcoliWiki:b0281 , ModBase:P71298 , OU-Microarray:b0281 , PortEco:intF , Pride:P71298 , Protein Model Portal:P71298 , RefSeq:NP_414815 , RegulonDB:G6152 , String:511145.b0281 , UniProt:P71298

Relationship Links: EcoO157Cyc:Homolog:Z1835 , EcoO157Cyc:Homolog:Z1835-MONOMER , InterPro:IN-FAMILY:IPR002104 , InterPro:IN-FAMILY:IPR011010 , InterPro:IN-FAMILY:IPR013762 , InterPro:IN-FAMILY:IPR025166 , Pfam:IN-FAMILY:PF00589 , Pfam:IN-FAMILY:PF13356

GO Terms:

Biological Process: GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0015074 - DNA integration Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0046718 - viral entry into host cell Inferred by computational analysis [UniProtGOA11a]
GO:0075713 - establishment of integrated proviral latency Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [GOA01a]

MultiFun Terms: extrachromosomal prophage genes and phage related functions

Essentiality data for intF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 18-Jan-2007 by Keseler I , SRI International

Sequence Features

Protein sequence of CP4-6 prophage; predicted phage integrase with features indicated

Feature Class Location Citations Comment
Active-Site 432
UniProt: O-(3'-phospho-DNA)-tyrosine intermediate; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc12.