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Metabolic Modeling Tutorial
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discounted EARLY registration ends Dec 31, 2014
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Escherichia coli K-12 substr. MG1655 Polypeptide: IS1 protein InsA



Gene: insA-2 Accession Numbers: G6139 (EcoCyc), b0265, ECK0023

Regulation Summary Diagram: ?

Summary:
IS1 is the smallest insertion sequence in E. coli. It codes for three proteins, InsA, InsB and InsAB'.

InsAB' is the transposase for IS1 [Escoubas94]. Its translation depends on a -1 frameshift within the 3' end region of insA which allows translational readthrough into insB [Sekine89]. In the absence of this frameshift, the IS1 transcriptional repressor protein InsA is produced instead [Sekine89, Escoubas94, Matsutani97].

Map Position: [278,824 <- 279,099] (6.01 centisomes)
Length: 276 bp / 91 aa

Molecular Weight of Polypeptide: 9.902 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000909 , EcoGene:EG60007 , EcoliWiki:b0265 , OU-Microarray:b0265 , PortEco:insA-2 , Protein Model Portal:P0CF08 , RefSeq:NP_414799 , RegulonDB:G6139 , SMR:P0CF08 , UniProt:P0CF08

Relationship Links: InterPro:IN-FAMILY:IPR003220 , InterPro:IN-FAMILY:IPR024431 , Pfam:IN-FAMILY:PF03811 , Pfam:IN-FAMILY:PF12759

GO Terms:

Biological Process: GO:0006313 - transposition, DNA-mediated Inferred from experiment Inferred by computational analysis [GOA01, Escoubas94]
GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11]
GO:0032196 - transposition Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: extrachromosomal transposon related

Credits:
Last-Curated ? 29-Aug-2006 by Shearer A , SRI International


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Escoubas94: Escoubas JM, Lane D, Chandler M (1994). "Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?." J Bacteriol 176(18);5864-7. PMID: 8083181

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Matsutani97: Matsutani S (1997). "Genetic analyses of the interactions of the IS1-encoded proteins with the left end of IS1 and its insertion hotspot." J Mol Biol 267(3);548-60. PMID: 9126837

Sekine89: Sekine Y, Ohtsubo E (1989). "Frameshifting is required for production of the transposase encoded by insertion sequence 1." Proc Natl Acad Sci U S A 86(12);4609-13. PMID: 2543983

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.