Escherichia coli K-12 substr. MG1655 Polypeptide: flagellar system protein, promoterless fragment

Gene: lfhA Accession Numbers: G6113 (EcoCyc), b0229, ECK0230

Synonyms: fhiA

Regulation Summary Diagram: ?

Regulation summary diagram for lfhA

Sequence analysis reveals fhiA and mbhA are two adjacent, divergent pseudogenes which represent the fused boundaries of a 44 gene cluster encoding a second flagellar system designated Flag-2 in E. coli strain 042. A few non-flagellar genes are also located within this region. The Flag-2 system has been deleted in K-12 leaving the fhiA-mbhA gene cluster promotorless. PCR experiments revealed that 15 out of 73 E. coli strains tested had the entire Flag-2 cluster, though it is believed to be inactive in these strains due to a frameshift mutation in the lfgC gene which encodes and FlgC-like proximal rod protein necessary for production of flagella. The Flag-2 locus has also been identified in a number of other bacteria including C. violaceum, C. rodentium, Y. pseudotuberculosis, Y. pestis, and V. parahaemolyticus [Ren05b].

Locations: inner membrane

Map Position: [248,358 <- 250,070] (5.35 centisomes, 19°)
Length: 1713 bp / 570 aa

Molecular Weight of Polypeptide: 62.154 kD (from nucleotide sequence)

Unification Links: DIP:DIP-9600N , EchoBASE:EB2938 , EcoGene:EG13144 , EcoliWiki:b0229 , OU-Microarray:b0229 , PortEco:lfhA , Pride:Q47153 , Protein Model Portal:Q47153 , RefSeq:NP_414764 , RegulonDB:G6113 , SMR:Q47153 , UniProt:Q47153

Relationship Links: InterPro:IN-FAMILY:IPR001712 , InterPro:IN-FAMILY:IPR025505 , Pfam:IN-FAMILY:PF00771 , Prints:IN-FAMILY:PR00949 , Prosite:IN-FAMILY:PS00994

GO Terms:

Biological Process: GO:0009306 - protein secretion Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

Gene Class: Pseudo-Genes

Essentiality data for lfhA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ren05b: Ren CP, Beatson SA, Parkhill J, Pallen MJ (2005). "The Flag-2 locus, an ancestral gene cluster, is potentially associated with a novel flagellar system from Escherichia coli." J Bacteriol 187(4);1430-40. PMID: 15687208

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, BIOCYC11A.