Escherichia coli K-12 substr. MG1655 Polypeptide: putative membrane fusion protein (MFP) component of efflux pump

Gene: yiaV Accession Numbers: EG12290 (EcoCyc), b3586, ECK3575

Regulation Summary Diagram: ?

Regulation summary diagram for yiaV

YiaV is an inner membrane protein with two predicted transmembrane domains. The C terminus is located in the periplasm [Daley05]. YiaV is a member of the Membrane Fusion Protein (MFP) Family [Saier14]

A transposon insertion in the yiaW-aldB intergenic region has higher tolerance to kanamycin, while a mutant lacking that region is more sensitive to kanamycin than wild type. A yiaV deletion has no effect on kanamycin sensitivity [Hu05].

Locations: inner membrane

Map Position: [3,750,986 <- 3,752,122] (80.85 centisomes, 291°)
Length: 1137 bp / 378 aa

Molecular Weight of Polypeptide: 41.771 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011712 , EchoBASE:EB2198 , EcoGene:EG12290 , EcoliWiki:b3586 , ModBase:P37683 , OU-Microarray:b3586 , PortEco:yiaV , Pride:P37683 , Protein Model Portal:P37683 , RefSeq:NP_418043 , RegulonDB:EG12290 , SMR:P37683 , String:511145.b3586 , UniProt:P37683

Relationship Links: InterPro:IN-FAMILY:IPR006143 , Pfam:IN-FAMILY:PF00529

In Paralogous Gene Group: 132 (13 members)

GO Terms:

Biological Process: GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]


Essentiality data for yiaV knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 21-Jun-2007 by Keseler I , SRI International

Sequence Features

Protein sequence of putative membrane fusion protein (MFP) component of efflux pump with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 15
UniProt: Non-Experimental Qualifier: potential;
Chain 16 -> 378
UniProt: Inner membrane protein yiaV;
Transmembrane-Region 27 -> 47
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 57 -> 77
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3586 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12290; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hu05: Hu Y, Coates AR (2005). "Transposon mutagenesis identifies genes which control antimicrobial drug tolerance in stationary-phase Escherichia coli." FEMS Microbiol Lett 243(1);117-24. PMID: 15668009

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.