Escherichia coli K-12 substr. MG1655 Enzyme: β-D-glucoside glucohydrolase, periplasmic

Gene: bglX Accession Numbers: EG12013 (EcoCyc), b2132, ECK2125

Synonyms: glh, yohA

Regulation Summary Diagram: ?

Regulation summary diagram for bglX

BglX is a periplasmic β-D-glucosidase.

The enzymatic activity of BglX was evaluated with the artificial substrate o-nitrophenyl β-D-glucopyranoside. It has a Km of 18mM and a Vmax of 3 μM/minute for this substrate. The actual, in vivo substrate of BglX is unknown. Deletion of bglX has no apparent effect, and overexpression of BglX does not aid growth on arbutin, salicin or β-D-cellobiose [Yang96b].

Locations: periplasmic space

Map Position: [2,217,714 <- 2,220,011] (47.8 centisomes, 172°)
Length: 2298 bp / 765 aa

Molecular Weight of Polypeptide: 83.46 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007043 , DIP:DIP-9218N , EchoBASE:EB1951 , EcoGene:EG12013 , EcoliWiki:b2132 , ModBase:P33363 , OU-Microarray:b2132 , PortEco:bglX , PR:PRO_000022219 , Pride:P33363 , Protein Model Portal:P33363 , RefSeq:NP_416636 , RegulonDB:EG12013 , SMR:P33363 , String:511145.b2132 , UniProt:P33363

Relationship Links: CAZy:IN-FAMILY:GH3 , InterPro:IN-FAMILY:IPR001764 , InterPro:IN-FAMILY:IPR002772 , InterPro:IN-FAMILY:IPR017853 , InterPro:IN-FAMILY:IPR019800 , InterPro:IN-FAMILY:IPR026891 , InterPro:IN-FAMILY:IPR026892 , Panther:IN-FAMILY:PTHR30620 , Pfam:IN-FAMILY:PF00933 , Pfam:IN-FAMILY:PF01915 , Pfam:IN-FAMILY:PF14310 , Prints:IN-FAMILY:PR00133 , Prosite:IN-FAMILY:PS00775

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [GOA01a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0008422 - beta-glucosidase activity Inferred from experiment Inferred by computational analysis [GOA01, Yang96b]
GO:0015926 - glucosidase activity Inferred from experiment [Yang96b]
GO:0004553 - hydrolase activity, hydrolyzing O-glycosyl compounds Inferred by computational analysis [GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016798 - hydrolase activity, acting on glycosyl bonds Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Zhang07, LopezCampistrou05, Yang96b, Han14]
GO:0042597 - periplasmic space Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Yang96b]

MultiFun Terms: metabolism carbon utilization carbon compounds

Essentiality data for bglX knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Last-Curated ? 11-May-2006 by Shearer A , SRI International
Revised 23-May-2011 by Brito D

Enzymatic reaction of: β-D-glucoside glucohydrolase

Synonyms: beta-D-glucoside glucohydrolase, periplasmic

EC Number:

a β-D glucoside[periplasmic space] + H2O[periplasmic space] <=> a non glucosylated D-glucose acceptor[periplasmic space] + β-D-glucopyranose[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Sequence Features

Protein sequence of beta-D-glucoside glucohydrolase, periplasmic with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 20
UniProt: Non-Experimental Qualifier: potential;
Chain 21 -> 765
UniProt: Periplasmic beta-glucosidase;
Active-Site 287
UniProt: Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b2132 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12013; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han14: Han MJ, Kim JY, Kim JA (2014). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng 117(4);437-42. PMID: 24140104

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yang96b: Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S (1996). "The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase." Microbiology 142 ( Pt 7);1659-65. PMID: 8757730

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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