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Escherichia coli K-12 substr. MG1655 Polypeptide: probable pilin chaperone similar to PapD

Gene: yadV Accession Numbers: EG11973 (EcoCyc), b0140, ECK0139

Synonyms: ecpD

Regulation Summary Diagram: ?

Regulation summary diagram for yadV

YadV (formerly EcpD) has similarity to PapD pilin chaperones [Raina93]. yadCKLMhtrEyadVN is a putative chaperone-usher fimbrial operon in E.coli K-12 [Korea10]. The operon is cryptic under normal laboratory conditions but when constitutive expression is induced in a strain that lacks the type 1 fimbrial complex the yad operon promotes biofilm formation in minimal media on a variety of abiotic surfaces and produces surface fimbrial structures that can be observed microscopically [Korea10]. Expression of the yad operon is negatively regulated by H-NS [Korea10].

Citations: [Korea11]

Locations: periplasmic space

Map Position: [155,461 <- 156,201] (3.35 centisomes, 12°)
Length: 741 bp / 246 aa

Molecular Weight of Polypeptide: 27.054 kD (from nucleotide sequence), 27 kD (experimental)

Unification Links: ASAP:ABE-0000482 , CGSC:30513 , DIP:DIP-9492N , EchoBASE:EB1916 , EcoGene:EG11973 , EcoliWiki:b0140 , Mint:MINT-1302192 , ModBase:P33128 , OU-Microarray:b0140 , PortEco:ecpD , PR:PRO_000022501 , Pride:P33128 , Protein Model Portal:P33128 , RefSeq:NP_414682 , RegulonDB:EG11973 , SMR:P33128 , String:511145.b0140 , UniProt:P33128

Relationship Links: InterPro:IN-FAMILY:IPR001829 , InterPro:IN-FAMILY:IPR008962 , InterPro:IN-FAMILY:IPR016147 , InterPro:IN-FAMILY:IPR016148 , InterPro:IN-FAMILY:IPR018046 , Pfam:IN-FAMILY:PF00345 , Pfam:IN-FAMILY:PF02753 , Prints:IN-FAMILY:PR00969 , Prosite:IN-FAMILY:PS00635

Genetic Regulation Schematic: ?

Genetic regulation schematic for yadV

GO Terms:

Biological Process: GO:0043711 - pilus organization Inferred by computational analysis [GOA01, Gaudet10]
GO:0061077 - chaperone-mediated protein folding Inferred by computational analysis [GOA01, Gaudet10]
GO:0071555 - cell wall organization Inferred by computational analysis [GOA01]
Molecular Function: GO:0044183 - protein binding involved in protein folding Inferred by computational analysis [Gaudet10]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [GOA01, DiazMejia09]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: cell structure pilus
information transfer protein related chaperoning, repair (refolding)

Essentiality data for yadV knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 14-Apr-2010 by Mackie A , Macquarie University

Sequence Features

Protein sequence of probable pilin chaperone similar to PapD with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 25
UniProt: Non-Experimental Qualifier: potential;
Chain 26 -> 246
UniProt: Chaperone protein ecpD;
Sequence-Conflict 79
[Raina93, UniProt10a]
UniProt: (in Ref. 1; AAA23720);
Sequence-Conflict 110 -> 111
[Raina93, UniProt10a]
UniProt: (in Ref. 1; AAA23720);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b0140 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11973; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Korea10: Korea CG, Badouraly R, Prevost MC, Ghigo JM, Beloin C (2010). "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities." Environ Microbiol 12(7):1957-77. PMID: 20345943

Korea11: Korea CG, Ghigo JM, Beloin C (2011). "The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile arsenal of sugar-binding lectins potentially involved in surface-colonisation and tissue tropism." Bioessays 33(4);300-11. PMID: 21344461

Raina93: Raina S, Missiakas D, Baird L, Kumar S, Georgopoulos C (1993). "Identification and transcriptional analysis of the Escherichia coli htrE operon which is homologous to pap and related pilin operons." J Bacteriol 1993;175(16);5009-21. PMID: 8102362

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Mon Aug 31, 2015, biocyc13.