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Escherichia coli K-12 substr. MG1655 Polypeptide: zinc homeostasis protein

Gene: zraP Accession Numbers: EG11918 (EcoCyc), b4002, ECK3994

Synonyms: yjaI, zra

Regulation Summary Diagram: ?

Regulation summary diagram for zraP

ZraP is a periplasmic protein that is involved in zinc homeostasis [Noll98]. ZraP binds zinc and, to a lesser extent, also binds cobalt and cadmium [Noll98].

ZraP has two zinc-binding motifs [Noll98].

Exogenous production of a transcriptional regulator from Proteus mirabilis causes zinc-specific induction of ZraP production that corresponds with greater zinc tolerance, compared to wild type [Noll98].

Regulation has been described [Noll98, Leonhartsberger01]. Transcription is induced by Zn2+ or Pb2+ [Leonhartsberger01] via the ZraS-ZraR two-component system [Leonhartsberger01].

Locations: periplasmic space

Map Position: [4,199,286 <- 4,199,711] (90.51 centisomes, 326°)
Length: 426 bp / 141 aa

Molecular Weight of Polypeptide: 15.199 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013081 , EchoBASE:EB1862 , EcoGene:EG11918 , EcoliWiki:b4002 , OU-Microarray:b4002 , PortEco:zraP , PR:PRO_000024255 , Protein Model Portal:P0AAA9 , RefSeq:NP_418430 , RegulonDB:EG11918 , SMR:P0AAA9 , String:511145.b4002 , UniProt:P0AAA9

Relationship Links: InterPro:IN-FAMILY:IPR020365 , InterPro:IN-FAMILY:IPR025961 , Pfam:IN-FAMILY:PF13801 , ProDom:IN-FAMILY:PD097062

Genetic Regulation Schematic: ?

Genetic regulation schematic for zraP

GO Terms:

Molecular Function: GO:0008270 - zinc ion binding Inferred from experiment Inferred by computational analysis [GOA01a, Sevcenco11]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [DiazMejia09]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA01a]

MultiFun Terms: cell processes adaptations other (mechanical, nutritional, oxidative stress)
transport Putative uncharacterized transport protein

Essentiality data for zraP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Protein sequence of zinc homeostasis protein with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 26
[Noll98, UniProt11]
Chain 27 -> 141
UniProt: Zinc resistance-associated protein;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


Martin Peralta on Fri Oct 29, 2004:
The start site of this gene was originally assigned solely on the basis of sequence considerations (Blattner et al, 1997, PubMed: 9278503). However, it was changed because Leonhartsberger et al (2001) attested that the real start site is actually located in another position (PMID:11243806). The demonstration is based on comparisons of the new gene sequence with homologous proteins, plus identification of both a plausible ribosome binding site and a promoter at an appropriate distance from the new start site.
10/20/97 Gene b4002 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11918; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Leonhartsberger01: Leonhartsberger S, Huber A, Lottspeich F, Bock A (2001). "The hydH/G Genes from Escherichia coli code for a zinc and lead responsive two-component regulatory system." J Mol Biol 2001;307(1);93-105. PMID: 11243806

Noll98: Noll M, Petrukhin K, Lutsenko S (1998). "Identification of a novel transcription regulator from Proteus mirabilis, PMTR, revealed a possible role of YJAI protein in balancing zinc in Escherichia coli." J Biol Chem 273(33);21393-401. PMID: 9694902

Sevcenco11: Sevcenco AM, Pinkse MW, Wolterbeek HT, Verhaert PD, Hagen WR, Hagedoorn PL (2011). "Exploring the microbial metalloproteome using MIRAGE." Metallomics 3(12);1324-30. PMID: 22094925

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Barrios99: Barrios H, Valderrama B, Morett E (1999). "Compilation and analysis of sigma(54)-dependent promoter sequences." Nucleic Acids Res 27(22);4305-13. PMID: 10536136

Ravikumar11: Ravikumar S, Yoo IK, Lee SY, Hong SH (2011). "A study on the dynamics of the zraP gene expression profile and its application to the construction of zinc adsorption bacteria." Bioprocess Biosyst Eng. PMID: 21674266

Reitzer01: Reitzer L, Schneider BL (2001). "Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli." Microbiol Mol Biol Rev 65(3);422-44, table of contents. PMID: 11528004

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Aug 28, 2015, biocyc13.