Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: predicted PTS permease - FrwD subunit



Gene: frwD Accession Numbers: EG11912 (EcoCyc), b3953, ECK3944

Synonyms: yijN, EIIB'frw, Enzyme IIB'frw

Regulation Summary Diagram: ?

Component of: predicted PTS permease FrwCBD (summary available)

Summary:
Sequence analysis indicates that an frwD encoded protein has similarity to the IIB domains of the PTS Enzymes II specific for fructose. frwD and frwB are similar to each other and to the IIB domains of the PTS Enzymes II specific for fructose. frwD contains conserved cysteine (cys10) and histidine residues (his16) predicted to be involved in phosphoryl transfer [Reizer95]

frwD is not expressed when cells are grown in rich medium [Wei01a].

Locations: cytosol

Map Position: [4,145,161 -> 4,145,502] (89.34 centisomes)
Length: 342 bp / 113 aa

Molecular Weight of Polypeptide: 12.637 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012941 , EchoBASE:EB1856 , EcoGene:EG11912 , EcoliWiki:b3953 , OU-Microarray:b3953 , PortEco:frwD , Pride:P32676 , Protein Model Portal:P32676 , RefSeq:NP_418388 , RegulonDB:EG11912 , SMR:P32676 , String:511145.b3953 , UniProt:P32676

Relationship Links: InterPro:IN-FAMILY:IPR003353 , InterPro:IN-FAMILY:IPR003501 , InterPro:IN-FAMILY:IPR013011 , Pfam:IN-FAMILY:PF02302 , Prosite:IN-FAMILY:PS51099

In Paralogous Gene Group: 414 (2 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, GOA01, Reizer95]
GO:0015755 - fructose transport Inferred by computational analysis [GOA01]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01a, GOA01]
Molecular Function: GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01a, GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0022877 - protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for frwD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: predicted PTS permease FrwCBD

Synonyms: EIIFrw, Enzyme IIFrw

Subunit composition of predicted PTS permease FrwCBD = [FrwC][FrwB][FrwD][PtsA]
         predicted PTS permease - FrwC subunit = FrwC (summary available)
         predicted PTS permease - FrwB subunit = FrwB (summary available)
         predicted PTS permease - FrwD subunit = FrwD (summary available)
         fused predicted PTS enzyme : HPr component / enzyme I component / enzyme IIA component = PtsA (extended summary available)

Summary:
FrwCBDPtsA is a putative PTS permease [Tchieu01] which belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its sugar substrates in a process called group translocation (reviewed by [Postma93]).

FrwC, FrwB, FrwD and PtsA (formerly FrwA) are all encoded within the frw gene cluster. The Frw proteins and protein domains are homologous to constituents of the fructose Enzyme II complexes. The frw gene cluster also encodes several enzymes concerned with anaerobic carbon metabolism [Reizer95]. FrwCBDPtsA is a member of the PTS Fructose-Mannitol (Fru) family of transporters [Saier14].

Please note that the presence of an frwCBDptsA encoded protein complex is hypothetical.

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [Reizer95, Tchieu01]

Credits:
Revised 30-Apr-2014 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 100
[UniProt09]
UniProt: PTS EIIB type-2;
Active-Site 10
[UniProt10, Reizer95]
UniProt: Phosphocysteine intermediate; Non-Experimental Qualifier: by similarity;
Amino-Acid-Site 16
[Reizer95]
conserved residue


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3953 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11912; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer95: Reizer J, Reizer A, Saier MH (1995). "Novel phosphotransferase system genes revealed by bacterial genome analysis--a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Microbiology 141 ( Pt 4);961-71. PMID: 7773398

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

Tchieu01: Tchieu JH, Norris V, Edwards JS, Saier MH (2001). "The complete phosphotransferase system in Escherichia coli." J Mol Microbiol Biotechnol 3(3);329-46. PMID: 11361063

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Wei01a: Wei Y, Lee JM, Richmond C, Blattner FR, Rafalski JA, LaRossa RA (2001). "High-density microarray-mediated gene expression profiling of Escherichia coli." J Bacteriol 183(2);545-56. PMID: 11133948


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc12.