Escherichia coli K-12 substr. MG1655 Enzyme: autophosphorylating protein tyrosine kinase

Gene: etk Accession Numbers: EG11826 (EcoCyc), b0981, ECK0972

Synonyms: yccC

Regulation Summary Diagram: ?

Regulation summary diagram for etk

Etk is a protein tyrosine kinase of the inner membrane that autophosphorylates and can be dephosphorylated by Etp [Ilan99, Vincent00, Klein03]. In contrast to the related kinase, Wzc, Etk has minimal effect on biosynthesis of colanic acid [Vincent00]. Etk, but not Wzc, plays a role in resistance to polymyxin B [Lacour06]. Etk phosphorylates the heat shock sigma factor σ32 in vitro [Klein03].

A crystal structure of the C-terminal kinase domain has been solved at 2.5 Å resolution. Etk shows structural similarity to the ATPase MinD [Lee08b].

Etk has two predicted transmembrane domains. The C terminus is located in the cytoplasm [Daley05].

The operon encoding YmcD, YmcC, YmcB, YmcA, YzzZ, Etp, and Etk was characterized in the enteropathogenic E. coli strain O127:H6. There, all seven gene products are required for the production of the G4C capsule polysaccharide [Peleg05]. That operon may not be expressed in E. coli K12, because the promoter region that was mapped in E. coli O127:H6 is inactivated in E. coli K12 by the insertion of an IS1 element 15 bp upstream of the YmcD translation start site [Ilan99, Peleg05].

Reviews: [Grangeasse07, Lee09g]

Locations: inner membrane

Map Position: [1,041,253 <- 1,043,433] (22.44 centisomes, 81°)
Length: 2181 bp / 726 aa

Molecular Weight of Polypeptide: 81.242 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0003309 , DIP:DIP-9526N , EchoBASE:EB1773 , EcoGene:EG11826 , EcoliWiki:b0981 , Mint:MINT-6743651 , ModBase:P38134 , OU-Microarray:b0981 , PortEco:etk , PR:PRO_000022533 , Pride:P38134 , Protein Model Portal:P38134 , RefSeq:NP_415501 , RegulonDB:EG11826 , SMR:P38134 , String:511145.b0981 , UniProt:P38134

Relationship Links: InterPro:IN-FAMILY:IPR003856 , InterPro:IN-FAMILY:IPR005702 , InterPro:IN-FAMILY:IPR025669 , InterPro:IN-FAMILY:IPR027417 , PDB:Structure:3CIO , Pfam:IN-FAMILY:PF02706 , Pfam:IN-FAMILY:PF13614

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0038083 - peptidyl-tyrosine autophosphorylation Inferred from experiment [Ilan99]
GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [GOA01a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
GO:0045226 - extracellular polysaccharide biosynthetic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0004713 - protein tyrosine kinase activity Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Ilan99]
GO:0042802 - identical protein binding Inferred from experiment [Lee08b]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016021 - integral component of membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, Ilan99]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
information transfer protein related posttranslational modification

Essentiality data for etk knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 17-Aug-2006 by Keseler I , SRI International

Enzymatic reaction of: protein-tyrosine kinase (autophosphorylating protein tyrosine kinase)

EC Number:

ATP + a [protein]-L-tyrosine <=> ADP + a [protein]-L-tyrosine phosphate + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

The reaction is physiologically favored in the direction shown.

Sequence Features

Protein sequence of autophosphorylating protein tyrosine kinase with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 33 -> 53
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 425 -> 445
UniProt: Helical;; Non-Experimental Qualifier: potential;
Phosphorylation-Modification 574
Sequence-Conflict 726
[Oshima96, UniProt10]
UniProt: (in Ref. 1);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b0981 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11826; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Grangeasse07: Grangeasse C, Cozzone AJ, Deutscher J, Mijakovic I (2007). "Tyrosine phosphorylation: an emerging regulatory device of bacterial physiology." Trends Biochem Sci 32(2);86-94. PMID: 17208443

Ilan99: Ilan O, Bloch Y, Frankel G, Ullrich H, Geider K, Rosenshine I (1999). "Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysaccharide." EMBO J 18(12);3241-8. PMID: 10369665

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Klein03: Klein G, Dartigalongue C, Raina S (2003). "Phosphorylation-mediated regulation of heat shock response in Escherichia coli." Mol Microbiol 48(1);269-85. PMID: 12657060

Lacour06: Lacour S, Doublet P, Obadia B, Cozzone AJ, Grangeasse C (2006). "A novel role for protein-tyrosine kinase Etk from Escherichia coli K-12 related to polymyxin resistance." Res Microbiol 157(7):637-41. PMID: 16814990

Lee08b: Lee DC, Zheng J, She YM, Jia Z (2008). "Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism." EMBO J 27(12):1758-66. PMID: 18497741

Lee09g: Lee DC, Jia Z (2009). "Emerging structural insights into bacterial tyrosine kinases." Trends Biochem Sci 34(7);351-7. PMID: 19525115

Oshima96: Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3(3);137-55. PMID: 8905232

Peleg05: Peleg A, Shifrin Y, Ilan O, Nadler-Yona C, Nov S, Koby S, Baruch K, Altuvia S, Elgrably-Weiss M, Abe CM, Knutton S, Saper MA, Rosenshine I (2005). "Identification of an Escherichia coli operon required for formation of the O-antigen capsule." J Bacteriol 187(15);5259-66. PMID: 16030220

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Vincent00: Vincent C, Duclos B, Grangeasse C, Vaganay E, Riberty M, Cozzone AJ, Doublet P (2000). "Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in gram-negative bacteria." J Mol Biol 304(3);311-21. PMID: 11090276

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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