Escherichia coli K-12 substr. MG1655 Polypeptide: member of ATP-dependent helicase superfamily II

Gene: lhr Accession Numbers: EG11548 (EcoCyc), b1653, ECK1649

Synonyms: rhlF

Regulation Summary Diagram: ?

Regulation summary diagram for lhr

Lhr codes for the longest known protein in E. coli. It has conserved motifs from helicase superfamily II, and its amino-terminus is similar to eukaryotic DEAD family helicases, though no DNA- or RNA-stimulated Lhr ATPase activity has so far been detected [Reuven95].

Mutations in lhr are synergistic with mutations in radA for sensitivity to the DNA damaging agent azidothymidine [Cooper15].

Gene Citations: [Rhodius05, Huang92]

Locations: inner membrane, cytosol

Map Position: [1,727,111 -> 1,731,727] (37.22 centisomes, 134°)
Length: 4617 bp / 1538 aa

Molecular Weight of Polypeptide: 169.38 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005525 , CGSC:35720 , DIP:DIP-10097N , EchoBASE:EB1510 , EcoGene:EG11548 , EcoliWiki:b1653 , Mint:MINT-1293547 , ModBase:P30015 , OU-Microarray:b1653 , PortEco:lhr , Pride:P30015 , Protein Model Portal:P30015 , RefSeq:NP_416170 , RegulonDB:EG11548 , SMR:P30015 , String:511145.b1653 , UniProt:P30015

Relationship Links: InterPro:IN-FAMILY:IPR001650 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR011545 , InterPro:IN-FAMILY:IPR013701 , InterPro:IN-FAMILY:IPR014001 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00270 , Pfam:IN-FAMILY:PF00271 , Pfam:IN-FAMILY:PF08494 , Prosite:IN-FAMILY:PS51192 , Prosite:IN-FAMILY:PS51194 , Smart:IN-FAMILY:SM00382 , Smart:IN-FAMILY:SM00487 , Smart:IN-FAMILY:SM00490

In Paralogous Gene Group: 20 (4 members) , 325 (2 members)

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA01]
GO:0004386 - helicase activity Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
GO:0016818 - hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]


Essentiality data for lhr knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of member of  ATP-dependent helicase superfamily II with features indicated

Feature Class Location Citations Comment
Conserved-Region 38 -> 235
UniProt: Helicase ATP-binding;
Nucleotide-Phosphate-Binding-Region 51 -> 58
UniProt: ATP; Non-Experimental Qualifier: by similarity;
Protein-Segment 179 -> 182
UniProt: DEVH box; Sequence Annotation Type: short sequence motif.
Conserved-Region 284 -> 462
UniProt: Helicase C-terminal;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b1653 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11548; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cooper15: Cooper DL, Boyle DC, Lovett ST (2015). "Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions." Mol Microbiol 95(5);769-79. PMID: 25484163

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Huang92: Huang S, Deutscher MP (1992). "Sequence and transcriptional analysis of the Escherichia coli rnt gene encoding RNase T." J Biol Chem 267(35);25609-13. PMID: 1460056

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Reuven95: Reuven NB, Koonin EV, Rudd KE, Deutscher MP (1995). "The gene for the longest known Escherichia coli protein is a member of helicase superfamily II." J Bacteriol 1995;177(19);5393-400. PMID: 7559321

Rhodius05: Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2005). "Conserved and variable functions of the sigmaE stress response in related genomes." PLoS Biol 4(1);e2. PMID: 16336047

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt15: UniProt Consortium (2015). "UniProt version 2015-01 released on 2015-01-16 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc14.