Escherichia coli K-12 substr. MG1655 Enzyme: methyltransferase for 50S ribosomal subunit protein L11

Gene: prmA Accession Numbers: EG11497 (EcoCyc), b3259, ECK3246

Synonyms: yhdI, prm-1, methylase for 50S ribosomal subunit protein L11, methyltransferase of 50S ribosomal subunit protein L11

Regulation Summary Diagram: ?

Regulation summary diagram for prmA

PrmA is the methyltransferase responsible for the multiple methylation of ribosomal protein L11.

prmA mutants are deficient in the methylation of ribosomal protein L11 [Colson77, Vanet94]. A strain containing a prmA null mutation is viable and shows no growth defect, indicating that lack of methylation of L11 does not affect its function in the ribosome [Vanet94].

PrmA: "protein methylation" [Colson77]

Gene Citations: [Vanet93]

Locations: cytosol

Map Position: [3,407,092 -> 3,407,973] (73.43 centisomes, 264°)
Length: 882 bp / 293 aa

Molecular Weight of Polypeptide: 31.877 kD (from nucleotide sequence), 31.0 kD (experimental) [Chang75a ]

Unification Links: ASAP:ABE-0010697 , CGSC:366 , DIP:DIP-47862N , EchoBASE:EB1460 , EcoGene:EG11497 , EcoliWiki:b3259 , Mint:MINT-1232840 , ModBase:P0A8T1 , OU-Microarray:b3259 , PortEco:prmA , PR:PRO_000023595 , Pride:P0A8T1 , Protein Model Portal:P0A8T1 , RefSeq:NP_417725 , RegulonDB:EG11497 , SMR:P0A8T1 , String:511145.b3259 , Swiss-Model:P0A8T1 , UniProt:P0A8T1

Relationship Links: InterPro:IN-FAMILY:IPR004498 , InterPro:IN-FAMILY:IPR010456 , InterPro:IN-FAMILY:IPR029063 , Panther:IN-FAMILY:PTHR18895:SF3 , Pfam:IN-FAMILY:PF06325

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0018012 - N-terminal peptidyl-alanine trimethylation Inferred from experiment [Colson77]
GO:0018023 - peptidyl-lysine trimethylation Inferred from experiment [Colson77, Chang75a]
GO:0006479 - protein methylation Inferred by computational analysis [GOA01a]
GO:0032259 - methylation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Arifuzzaman06, Butland05]
GO:0016279 - protein-lysine N-methyltransferase activity Inferred from experiment [Chang75a]
GO:0008168 - methyltransferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0008276 - protein methyltransferase activity Inferred by computational analysis [GOA06, GOA01a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08]
GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06]

MultiFun Terms: information transfer protein related posttranslational modification
information transfer protein related ribosomal proteins

Essentiality data for prmA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 07-Aug-2008 by Keseler I , SRI International

Enzymatic reaction of: ribosomal protein L11 methyltransferase (methyltransferase for 50S ribosomal subunit protein L11)

a non-methylated ribosomal protein L11 + S-adenosyl-L-methionine <=> a methylated ribosomal protein L11 + S-adenosyl-L-homocysteine

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Kinetic Parameters:

Km (μM)

pH(opt): 7.8-8.2 [Chang75a]

Sequence Features

Protein sequence of methyltransferase for 50S ribosomal subunit protein L11 with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 78
[Vanet93, UniProt10a]
UniProt: (in Ref. 1; AAB28769);
Mutagenesis-Variant 285
[Vanet94, UniProt11]
UniProt: Great decrease in activity.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3259 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11497; confirmed by SwissProt match.


Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Chang75a: Chang FN, Cohen LB, Navickas IJ, Chang CN (1975). "Purification and properties of a ribosomal protein methylase from Eschericha coli Q13." Biochemistry 14(22);4994-8. PMID: 241395

Colson77: Colson C, Smith HO (1977). "Genetics of ribosomal protein methylation in Escherichia coli. I. A mutant deficient in methylation of protein L11." Mol. Gen. Genet. 154:167-173. PMID: 331082

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Vanet93: Vanet A, Plumbridge JA, Alix JH (1993). "Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12." J Bacteriol 1993;175(22);7178-88. PMID: 8226664

Vanet94: Vanet A, Plumbridge JA, Guerin MF, Alix JH (1994). "Ribosomal protein methylation in Escherichia coli: the gene prmA, encoding the ribosomal protein L11 methyltransferase, is dispensable." Mol Microbiol 14(5);947-58. PMID: 7715456

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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