Escherichia coli K-12 substr. MG1655 Polypeptide: predicted diguanylate cyclase

Gene: adrA Accession Numbers: EG11257 (EcoCyc), b0385, ECK0380

Synonyms: yaiC

Regulation Summary Diagram: ?

Regulation summary diagram for adrA

Cellulose production is a common trait in wild isolates of E. coli, but not in K-12 strains [Da06, Zogaj01]. AdrA is part of a regulatory network involved in development of curli that is not active in the MG1655 strain due to lack of production of the upstream regulator CsgD [Brombacher03]. AdrA production induces biosynthesis of cellulose in some E. coli isolates (but not in K-12 strains); cellulose is observed in the extracellular matrix (ECM) of the multicellular (rdar) morphotype [Zogaj01].

AdrA has an N-terminal MASE2 (Membrane-Associated SEnsor) domain, which includes six putative membrane segments and is found in proteins associated with signal transduction. The MASE2 domain is followed by a predicted diguanylate cyclase (GGDEF) domain [Nikolskaya03].

Increased expression of csgD, which encodes a transcriptional activator of curli production, stimulates expression of adrA [Brombacher03].

Review: [Romling05]

Locations: inner membrane

Map Position: [402,927 -> 404,042] (8.68 centisomes, 31°)
Length: 1116 bp / 371 aa

Molecular Weight of Polypeptide: 41.537 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001336 , EchoBASE:EB1237 , EcoGene:EG11257 , EcoliWiki:b0385 , OU-Microarray:b0385 , PortEco:adrA , Pride:P0AAP1 , Protein Model Portal:P0AAP1 , RefSeq:NP_414919 , RegulonDB:EG11257 , SMR:P0AAP1 , String:511145.b0385 , UniProt:P0AAP1

Relationship Links: InterPro:IN-FAMILY:IPR000160 , InterPro:IN-FAMILY:IPR007894 , InterPro:IN-FAMILY:IPR029787 , Pfam:IN-FAMILY:PF00990 , Pfam:IN-FAMILY:PF05230 , Prosite:IN-FAMILY:PS50887 , Smart:IN-FAMILY:SM00267

In Paralogous Gene Group: 117 (13 members)

Genetic Regulation Schematic: ?

Genetic regulation schematic for adrA

GO Terms:

Biological Process: GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a, GOA01]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005525 - GTP binding Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
GO:0052621 - diguanylate cyclase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
regulation type of regulation unknown

Essentiality data for adrA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 01-Sep-2006 by Keseler I , SRI International

Sequence Features

Protein sequence of predicted diguanylate cyclase with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 46 -> 66
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 68 -> 88
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 112 -> 132
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 143 -> 163
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 171 -> 191
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 240 -> 371
UniProt: GGDEF;
Metal-Binding-Site 248
UniProt: Magnesium; Non-Experimental Qualifier: by similarity;
Amino-Acid-Site 253
UniProt: Transition state stabilizer; Sequence Annotation Type: site; Non-Experimental Qualifier: potential;
Amino-Acid-Sites-That-Bind 256
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Amino-Acid-Sites-That-Bind 265
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Mutagenesis-Variant 291 -> 292
[Antoniani10, UniProt11]
UniProt: No c-di-GMP produced in an overexpressing strain.
Metal-Binding-Site 291
UniProt: Magnesium; Non-Experimental Qualifier: by similarity;
Active-Site 291
UniProt: Proton acceptor; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b0385 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11257; confirmed by SwissProt match.


Antoniani10: Antoniani D, Bocci P, Maciag A, Raffaelli N, Landini P (2010). "Monitoring of diguanylate cyclase activity and of cyclic-di-GMP biosynthesis by whole-cell assays suitable for high-throughput screening of biofilm inhibitors." Appl Microbiol Biotechnol 85(4);1095-104. PMID: 19707751

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brombacher03: Brombacher E, Dorel C, Zehnder AJ, Landini P (2003). "The curli biosynthesis regulator CsgD co-ordinates the expression of both positive and negative determinants for biofilm formation in Escherichia coli." Microbiology 149(Pt 10);2847-57. PMID: 14523117

Da06: Da Re S, Ghigo JM (2006). "A CsgD-independent pathway for cellulose production and biofilm formation in Escherichia coli." J Bacteriol 188(8);3073-87. PMID: 16585767

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Nikolskaya03: Nikolskaya AN, Mulkidjanian AY, Beech IB, Galperin MY (2003). "MASE1 and MASE2: two novel integral membrane sensory domains." J Mol Microbiol Biotechnol 5(1);11-6. PMID: 12673057

Romling05: Romling U (2005). "Characterization of the rdar morphotype, a multicellular behaviour in Enterobacteriaceae." Cell Mol Life Sci 62(11);1234-46. PMID: 15818467

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zogaj01: Zogaj X, Nimtz M, Rohde M, Bokranz W, Romling U (2001). "The multicellular morphotypes of Salmonella typhimurium and Escherichia coli produce cellulose as the second component of the extracellular matrix." Mol Microbiol 39(6);1452-63. PMID: 11260463

Other References Related to Gene Regulation

Ogasawara11: Ogasawara H, Yamamoto K, Ishihama A (2011). "Role of the Biofilm Master Regulator CsgD in Cross-Regulation between Biofilm Formation and Flagellar Synthesis." J Bacteriol 193(10);2587-97. PMID: 21421764

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc12.