Escherichia coli K-12 substr. MG1655 Polypeptide: UvrY transcriptional regulator

Gene: uvrY Accession Numbers: EG11140 (EcoCyc), b1914, ECK1913

Synonyms: yecB, UvrY response regulator

Regulation Summary Diagram: ?

Regulation summary diagram for uvrY

Alternative forms of UvrY transcriptional regulator: UvrY- Phosphorylated transcriptional regulator

The protein belongs to the LuxR/UhpA family. This regulator participates in controlling several genes involved in the DNA repair system and carbon metabolism.

UvrY has been shown to be the cognate response regulator for the BarA sensor protein. The genes regulated by this two-component sytem have yet to be identified. The signal the system responds to is also not known, but may be associated with hydrogen peroxide-mediated stress [Pernestig01].

Gene Citations: [Moolenaar87]

Locations: cytosol

Map Position: [1,992,727 <- 1,993,383] (42.95 centisomes, 155°)
Length: 657 bp / 218 aa

Molecular Weight of Polypeptide: 23.893 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006373 , DIP:DIP-35922N , EchoBASE:EB1130 , EcoGene:EG11140 , EcoliWiki:b1914 , Mint:MINT-1243107 , ModBase:P0AED5 , OU-Microarray:b1914 , PortEco:uvrY , PR:PRO_000024202 , Pride:P0AED5 , Protein Model Portal:P0AED5 , RegulonDB:EG11140 , SMR:P0AED5 , String:511145.b1914 , UniProt:P0AED5

Relationship Links: InterPro:IN-FAMILY:IPR000792 , InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR016032 , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF00196 , Prints:IN-FAMILY:PR00038 , Prosite:IN-FAMILY:PS00622 , Prosite:IN-FAMILY:PS50043 , Prosite:IN-FAMILY:PS50110 , Smart:IN-FAMILY:SM00421 , Smart:IN-FAMILY:SM00448

In Paralogous Gene Group: 121 (40 members)

Reactions known to consume the compound:

BarA UvrY Two-Component Signal Transduction System :
UvrY + BarA-Phis816 → UvrY-Pasp54 + BarA

Genetic Regulation Schematic: ?

Genetic regulation schematic for uvrY

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Pernestig01]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0000156 - phosphorelay response regulator activity Inferred from experiment [Pernestig01]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
regulation type of regulation transcriptional level activator

Essentiality data for uvrY knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of UvrY transcriptional regulator with features indicated

Feature Class Location Citations Comment State
Conserved-Region 1 -> 119
UniProt: Response regulatory;
Sequence-Conflict 8 -> 9
[Sharma86, UniProt10]
UniProt: (in Ref. 5; CAA27328);
4-aspartylphosphate-Modification 54
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
Conserved-Region 143 -> 208
UniProt: HTH luxR-type;
DNA-Binding-Region 167 -> 186
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b1914 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11140; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Moolenaar87: Moolenaar GF, van Sluis CA, Backendorf C, van de Putte P (1987). "Regulation of the Escherichia coli excision repair gene uvrC. Overlap between the uvrC structural gene and the region coding for a 24 kD protein." Nucleic Acids Res 1987;15(10);4273-89. PMID: 3295776

Pernestig01: Pernestig AK, Melefors O, Georgellis D (2001). "Identification of UvrY as the cognate response regulator for the BarA sensor kinase in Escherichia coli." J Biol Chem 2001;276(1);225-31. PMID: 11022030

Pernestig03: Pernestig AK, Georgellis D, Romeo T, Suzuki K, Tomenius H, Normark S, Melefors O (2003). "The Escherichia coli BarA-UvrY two-component system is needed for efficient switching between glycolytic and gluconeogenic carbon sources." J Bacteriol 185(3);843-53. PMID: 12533459

Sharma86: Sharma S, Stark TF, Beattie WG, Moses RE (1986). "Multiple control elements for the uvrC gene unit of Escherichia coli." Nucleic Acids Res 14(5);2301-18. PMID: 3515318

Suzuki02b: Suzuki K, Wang X, Weilbacher T, Pernestig AK, Melefors O, Georgellis D, Babitzke P, Romeo T (2002). "Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli." J Bacteriol 184(18);5130-40. PMID: 12193630

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11a: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MedinaRivera11: Medina-Rivera A, Abreu-Goodger C, Thomas-Chollier M, Salgado H, Collado-Vides J, van Helden J (2011). "Theoretical and empirical quality assessment of transcription factor-binding motifs." Nucleic Acids Res 39(3);808-24. PMID: 20923783

Stark89: Stark T, Moses RE (1989). "Interaction of the LexA repressor and the uvrC regulatory region." FEBS Lett 258(1);39-41. PMID: 2556297

Vakulskas14: Vakulskas CA, Pannuri A, Cortes-Selva D, Zere TR, Ahmer BM, Babitzke P, Romeo T (2014). "Global effects of the DEAD-box RNA helicase DeaD (CsdA) on gene expression over a broad range of temperatures." Mol Microbiol 92(5);945-58. PMID: 24708042

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Oct 8, 2015, biocyc13.