Escherichia coli K-12 substr. MG1655 Polypeptide: ferric enterobactin (enterochelin) transport

Gene: fepE Accession Numbers: EG10297 (EcoCyc), b0587, ECK0580

Regulation Summary Diagram: ?

Regulation summary diagram for fepE

FepE is believed to be a component of the ferric enterobactin transport system, because mutation of fepE disrupts uptake of enterobactin [Ozenberger87]. A fepE homolog in Salmonella enterica enterica serovar Typhimurium has been shown to be responsible for very long O antigen polymerization in lipopolysaccharide, providing minor complement resistance and significant serum resistance in mouse infection models [Murray03, Murray05a], and it was shown to be upregulated during swarming [Wang04a]. fepE was shown to be regulated by the FlhDC flagellar transcriptional regulator [Stafford05].

Gene Citations: [Hunt94]

Locations: inner membrane

Map Position: [617,477 -> 618,610] (13.31 centisomes, 48°)
Length: 1134 bp / 377 aa

Molecular Weight of Polypeptide: 42.1 kD (from nucleotide sequence), 43 kD (experimental) [Ozenberger87 ]

Unification Links: ASAP:ABE-0002023 , CGSC:18379 , DIP:DIP-9596N , EchoBASE:EB0293 , EcoGene:EG10297 , EcoliWiki:b0587 , OU-Microarray:b0587 , PortEco:fepE , PR:PRO_000022604 , Protein Model Portal:P26266 , RefSeq:NP_415119 , RegulonDB:EG10297 , SMR:P26266 , String:511145.b0587 , UniProt:P26266

Relationship Links: InterPro:IN-FAMILY:IPR003856 , Pfam:IN-FAMILY:PF02706

In Paralogous Gene Group: 158 (3 members)

Genetic Regulation Schematic: ?

Genetic regulation schematic for fepE

GO Terms:

Biological Process: GO:0015685 - ferric-enterobactin transport Inferred from experiment [Ozenberger87]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11a]
GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [GOA01a]
GO:0055072 - iron ion homeostasis Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0015620 - ferric-enterobactin transmembrane transporter activity Inferred from experiment [Ozenberger87]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Dux87]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes adaptations Fe aquisition
cell structure membrane

Essentiality data for fepE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of ferric enterobactin (enterochelin) transport with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 42 -> 62
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 339 -> 359
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b0587 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10297; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dux87: Dux L, Pikula S, Mullner N, Martonosi A (1987). "Crystallization of Ca2+-ATPase in detergent-solubilized sarcoplasmic reticulum." J Biol Chem 262(14);6439-42. PMID: 2952650

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hunt94: Hunt MD, Pettis GS, McIntosh MA (1994). "Promoter and operator determinants for fur-mediated iron regulation in the bidirectional fepA-fes control region of the Escherichia coli enterobactin gene system." J Bacteriol 176(13);3944-55. PMID: 8021177

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Murray03: Murray GL, Attridge SR, Morona R (2003). "Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz." Mol Microbiol 47(5);1395-406. PMID: 12603743

Murray05a: Murray GL, Attridge SR, Morona R (2005). "Inducible serum resistance in Salmonella typhimurium is dependent on wzz(fepE)-regulated very long O antigen chains." Microbes Infect 7(13);1296-304. PMID: 16027021

Ozenberger87: Ozenberger BA, Nahlik MS, McIntosh MA (1987). "Genetic organization of multiple fep genes encoding ferric enterobactin transport functions in Escherichia coli." J Bacteriol 169(8);3638-46. PMID: 2956250

Stafford05: Stafford GP, Ogi T, Hughes C (2005). "Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2." Microbiology 151(Pt 6);1779-88. PMID: 15941987

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wang04a: Wang Q, Frye JG, McClelland M, Harshey RM (2004). "Gene expression patterns during swarming in Salmonella typhimurium: genes specific to surface growth and putative new motility and pathogenicity genes." Mol Microbiol 52(1);169-87. PMID: 15049819

Other References Related to Gene Regulation

Chen07: Chen Z, Lewis KA, Shultzaberger RK, Lyakhov IG, Zheng M, Doan B, Storz G, Schneider TD (2007). "Discovery of Fur binding site clusters in Escherichia coli by information theory models." Nucleic Acids Res 35(20);6762-77. PMID: 17921503

Constantinidou06: Constantinidou C, Hobman JL, Griffiths L, Patel MD, Penn CW, Cole JA, Overton TW (2006). "A reassessment of the FNR regulon and transcriptomic analysis of the effects of nitrate, nitrite, NarXL, and NarQP as Escherichia coli K12 adapts from aerobic to anaerobic growth." J Biol Chem 281(8);4802-15. PMID: 16377617

Escolar98: Escolar L, Perez-Martin J, de Lorenzo V (1998). "Coordinated repression in vitro of the divergent fepA-fes promoters of Escherichia coli by the iron uptake regulation (Fur) protein." J Bacteriol 180(9);2579-82. PMID: 9573216

Kumar11: Kumar R, Shimizu K (2011). "Transcriptional regulation of main metabolic pathways of cyoA, cydB, fnr, and fur gene knockout Escherichia coli in C-limited and N-limited aerobic continuous cultures." Microb Cell Fact 10;3. PMID: 21272324

Newman99: Newman DL, Shapiro JA (1999). "Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development." Mol Microbiol 33(1);18-32. PMID: 10411720

Pettis88: Pettis GS, Brickman TJ, McIntosh MA (1988). "Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter." J Biol Chem 263(35);18857-63. PMID: 2974033

Vassinova00: Vassinova N, Kozyrev D (2000). "A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli." Microbiology 146 Pt 12;3171-82. PMID: 11101675

WhiteZiegler07: White-Ziegler CA, Malhowski AJ, Young S (2007). "Human body temperature (37degrees C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12." J Bacteriol 189(15);5429-40. PMID: 17526711

Zhang05: Zhang Z, Gosset G, Barabote R, Gonzalez CS, Cuevas WA, Saier MH (2005). "Functional interactions between the carbon and iron utilization regulators, Crp and Fur, in Escherichia coli." J Bacteriol 187(3);980-90. PMID: 15659676

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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