Escherichia coli K-12 substr. MG1655 Polypeptide: predicted inner membrane protein

Gene: ampE Accession Numbers: EG10042 (EcoCyc), b0111, ECK0110

Regulation Summary Diagram: ?

Regulation summary diagram for ampE

ampE encodes a cytoplasmic membrane protein believed to be involved in sensory transduction targeted to reduce expression of the β-lactamase producing ampC gene through the AmpR regulator, which Escherichia coli does not possess [Lindquist89]. Deletion of ampD and ampE resulted in overexpression of AmpC β-lactamase when ampC and ampR were provided from Citrobacter freundii [Lindquist89]. When AmpE was provided in the ampD ampE mutant, expression of ampC returned to its normal level, but was hyperinducible [Lindquist89].

Review: [Bennett93]

Gene Citations: [Honore89]

Locations: inner membrane

Map Position: [119,281 -> 120,135] (2.57 centisomes, 9°)
Length: 855 bp / 284 aa

Molecular Weight of Polypeptide: 32.122 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000382 , CGSC:30481 , DIP:DIP-47964N , EchoBASE:EB0040 , EcoGene:EG10042 , EcoliWiki:b0111 , OU-Microarray:b0111 , PortEco:ampE , PR:PRO_000022097 , Protein Model Portal:P0AE14 , RefSeq:NP_414653 , RegulonDB:EG10042 , String:511145.b0111 , UniProt:P0AE14

Genetic Regulation Schematic: ?

Genetic regulation schematic for ampE

GO Terms:

Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Lindquist89]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell processes protection drug resistance/sensitivity
cell structure membrane

Essentiality data for ampE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of predicted inner membrane protein with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 44 -> 60
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 68 -> 84
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 195 -> 211
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 268 -> 284
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b0111 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10042; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bennett93: Bennett PM, Chopra I (1993). "Molecular basis of beta-lactamase induction in bacteria." Antimicrob Agents Chemother 37(2);153-8. PMID: 8452343

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Honore89: Honore N, Nicolas MH, Cole ST (1989). "Regulation of enterobacterial cephalosporinase production: the role of a membrane-bound sensory transducer." Mol Microbiol 1989;3(8);1121-30. PMID: 2607970

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lindquist89: Lindquist S, Galleni M, Lindberg F, Normark S (1989). "Signalling proteins in enterobacterial AmpC beta-lactamase regulation." Mol Microbiol 3(8);1091-102. PMID: 2691840

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Raghavan11: Raghavan R, Sage A, Ochman H (2011). "Genome-wide identification of transcription start sites yields a novel thermosensing RNA and new cyclic AMP receptor protein-regulated genes in Escherichia coli." J Bacteriol 193(11);2871-4. PMID: 21460078

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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