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Escherichia coli K-12 substr. MG1655 Protein: TreR-trehalose 6-phosphate



Gene: treR Accession Numbers: EG12202 (EcoCyc), b4241, ECK4236

Regulation Summary Diagram: ?

Subunit composition of TreR-trehalose 6-phosphate = [TreR][α,α-trehalose 6-phosphate]
         TreR transcriptional repressor = TreR

Citations: [Horlacher97]

Gene Citations: [Horlacher97]

Map Position: [4,464,322 <- 4,465,269] (96.22 centisomes)
Length: 948 bp / 315 aa

Unification Links: ASAP:ABE-0013875 , EchoBASE:EB2118 , EcoGene:EG12202 , OU-Microarray:b4241 , PortEco:treR , RegulonDB:EG12202

In Paralogous Gene Group: 30 (25 members)

In Reactions of unknown directionality:

Not in pathways:
TreR + α,α-trehalose 6-phosphate = TreR-trehalose 6-phosphate

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

MultiFun Terms: cell processes adaptations osmotic pressure
information transfer RNA related Transcription related
metabolism central intermediary metabolism misc. glucose metabolism
regulation genetic unit regulated regulon
regulation type of regulation transcriptional level repressor


Subunit of TreR-trehalose 6-phosphate: TreR transcriptional repressor

Synonyms: TreR

Gene: treR Accession Numbers: EG12202 (EcoCyc), b4241, ECK4236

Locations: cytosol

Sequence Length: 315 AAs

Molecular Weight: 34.531 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0045892 - negative regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [GOA01a, Klein95, Horlacher97]
GO:0005991 - trehalose metabolic process Inferred by computational analysis [GOA01a]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred from experiment Inferred by computational analysis [GOA01a, Horlacher97, Klein95]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
Cellular Component: GO:0005737 - cytoplasm Inferred by curator
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: cell processes adaptations osmotic pressure
information transfer RNA related Transcription related
metabolism central intermediary metabolism misc. glucose metabolism
regulation genetic unit regulated regulon
regulation type of regulation transcriptional level repressor

Unification Links: DIP:DIP-11026N , EcoliWiki:b4241 , ModBase:P36673 , PR:PRO_000024110 , Pride:P36673 , Protein Model Portal:P36673 , RefSeq:NP_418662 , SMR:P36673 , String:511145.b4241 , UniProt:P36673

Relationship Links: InterPro:IN-FAMILY:IPR000843 , InterPro:IN-FAMILY:IPR010982 , InterPro:IN-FAMILY:IPR012771 , InterPro:IN-FAMILY:IPR028082 , PDB:Structure:1BYK , Pfam:IN-FAMILY:PF00356 , Prints:IN-FAMILY:PR00036 , Prosite:IN-FAMILY:PS00356 , Prosite:IN-FAMILY:PS50932 , Smart:IN-FAMILY:SM00354

Essentiality data for treR knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b4241 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12202; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Horlacher97: Horlacher R, Boos W (1997). "Characterization of TreR, the major regulator of the Escherichia coli trehalose system." J Biol Chem 1997;272(20);13026-32. PMID: 9148912

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Klein95: Klein W, Horlacher R, Boos W (1995). "Molecular analysis of treB encoding the Escherichia coli enzyme II specific for trehalose." J Bacteriol 177(14);4043-52. PMID: 7608078

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Kato99a: Kato A, Tanabe H, Utsumi R (1999). "Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters." J Bacteriol 1999;181(17);5516-20. PMID: 10464230

Minagawa03: Minagawa S, Ogasawara H, Kato A, Yamamoto K, Eguchi Y, Oshima T, Mori H, Ishihama A, Utsumi R (2003). "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." J Bacteriol 185(13);3696-702. PMID: 12813061

Wade06: Wade JT, Roa DC, Grainger DC, Hurd D, Busby SJ, Struhl K, Nudler E (2006). "Extensive functional overlap between sigma factors in Escherichia coli." Nat Struct Mol Biol 13(9);806-14. PMID: 16892065

Yamamoto02: Yamamoto K, Ogasawara H, Fujita N, Utsumi R, Ishihama A (2002). "Novel mode of transcription regulation of divergently overlapping promoters by PhoP, the regulator of two-component system sensing external magnesium availability." Mol Microbiol 45(2);423-38. PMID: 12123454


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, biocyc14.