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Escherichia coli K-12 substr. MG1655 Enzyme: trimethylamine N-oxide reductase TorYZ

Synonyms: menaquinol:trimethylamine N-oxide oxidoreductase

Subunit composition of trimethylamine N-oxide reductase TorYZ = [TorZ][TorY]
         trimethylamine N-oxide reductase, TorZ subunit = TorZ (summary available)
         trimethylamine N-oxide reductase, c-type cytochrome subunit = TorY (summary available)

Summary:
torYZ encodes a trimethylamine N-oxide (TMAO) reductase complex in E. coli K-12. TorZ is the catalytic subunit and TorY the pentahemic c-type cytochrome subunit. The enzyme has broad substrate specificity; it is able to reduce N- and S-oxide compounds. TMAO is the best substrate for the enzyme; dimethyl sulfoxide (DMSO) is a weak substrate in vitro; DMSO is a competitive inhibitor [Gon00]

Expression of torYZ is very low and not inducible by TMAO or dimethyl sulfoxide [Gon00].

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Molecular Function: GO:0050626 - trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from experiment [Gon00]

Credits:
Last-Curated ? 27-Nov-2014 by Mackie A , Macquarie University


Enzymatic reaction of: trimethylamine N-oxide reductase

Synonyms: TMAO reductase, biotin sulfoxide reductase

Transport reaction diagram for trimethylamine N-oxide reductase

Alternative Substrates for trimethylamine N-oxide: methionine S-oxide [Gon00 ] , tetrahydrothiophene 1-oxide [Gon00 ] , 4-methylmorpholine N-Oxide [Gon00 ] , d-biotin d-sulfoxide [Gon00 ]

Summary:

Inhibitors (Competitive): dimethyl sulfoxide [Gon00]

Kinetic Parameters:

Substrate
Km (μM)
kcat (sec-1)
kcat/Km (sec-1 μM-1)
Citations
trimethylamine N-oxide
670.0, 950.0
[Yamamoto86, BRENDA14]
trimethylamine N-oxide
1440.0
[Gon00, BRENDA14]
trimethylamine N-oxide
70.0
151.0
2.16
[IobbiNivol96, BRENDA14]
trimethylamine N-oxide
31.6, 7.65, 27.8
58.0, 114.0, 258.0
1.84, 3.61, 8.16, 7.58, 14.9, 33.73, 2.09, 4.1, 9.28
[Buc99, BRENDA14]
4-methylmorpholine N-Oxide
1400.0
[Gon00]
tetrahydrothiophene 1-oxide
3370.0
[Gon00]
methionine S-oxide
800.0
[Gon00]
d-biotin d-sulfoxide
360.0
[Gon00]

T(opt): 80 °C [BRENDA14, Buc99]

pH(opt): 6.9 [BRENDA14, Yamamoto86]


Subunit of trimethylamine N-oxide reductase TorYZ: trimethylamine N-oxide reductase, TorZ subunit

Synonyms: TorZ, BisZ

Gene: torZ Accession Numbers: G7022 (EcoCyc), b1872, ECK1873

Locations: periplasmic space

Sequence Length: 809 AAs

Molecular Weight: 88.964 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0009061 - anaerobic respiration Inferred by computational analysis [Gaudet10]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0050626 - trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from experiment Inferred by computational analysis [GOA01, Gon00]
GO:0003954 - NADH dehydrogenase activity Inferred by computational analysis [Gaudet10]
GO:0009055 - electron carrier activity Inferred by computational analysis [Gaudet10, GOA01a]
GO:0016491 - oxidoreductase activity Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0030151 - molybdenum ion binding Inferred by computational analysis [Gaudet10, GOA01a]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [Gaudet10, Gon00, DiazMejia09]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolism energy metabolism, carbon anaerobic respiration
metabolism energy production/transport electron carriers

Unification Links: DIP:DIP-9226N , EcoliWiki:b1872 , ModBase:P46923 , PR:PRO_000024093 , Pride:P46923 , Protein Model Portal:P46923 , RefSeq:NP_416386 , SMR:P46923 , String:511145.b1872 , Swiss-Model:P46923 , UniProt:P46923

Relationship Links: InterPro:IN-FAMILY:IPR006311 , InterPro:IN-FAMILY:IPR006655 , InterPro:IN-FAMILY:IPR006656 , InterPro:IN-FAMILY:IPR006657 , InterPro:IN-FAMILY:IPR006658 , InterPro:IN-FAMILY:IPR009010 , InterPro:IN-FAMILY:IPR019546 , Pfam:IN-FAMILY:PF00384 , Pfam:IN-FAMILY:PF01568 , Prosite:IN-FAMILY:PS00490 , Prosite:IN-FAMILY:PS00932 , Prosite:IN-FAMILY:PS51318

Summary:
The TorZ protein was first identified as a biotin sulfoxide (BSO) reductase, and the gene was named bisZ [delCampillo96]. Recent work has shown that the reductase is more efficient with TMAO as a substrate than BSO. TorZ is exported to the periplasm via the Tat pathway. TorZ has 62% identity with the cytoplasmic BSO reductase and 42% identity with TorA [Gon00].

Essentiality data for torZ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of trimethylamine N-oxide reductase TorYZ: trimethylamine N-oxide reductase, c-type cytochrome subunit

Synonyms: YecK, TorY

Gene: torY Accession Numbers: G7023 (EcoCyc), b1873, ECK1874

Locations: cell wall, inner membrane

Sequence Length: 366 AAs

Molecular Weight: 40.286 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0009061 - anaerobic respiration Inferred by computational analysis [Gaudet10]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005506 - iron ion binding Inferred by computational analysis [GOA01a]
GO:0009055 - electron carrier activity Inferred by computational analysis [Gaudet10, GOA01a]
GO:0020037 - heme binding Inferred by computational analysis [GOA01a, Gon00]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005887 - integral component of plasma membrane Inferred from experiment [Gon00]
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09]
GO:0009276 - Gram-negative-bacterium-type cell wall Inferred by computational analysis [GOA01a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, Gaudet10, GOA01a]

MultiFun Terms: metabolism energy metabolism, carbon anaerobic respiration
metabolism energy production/transport electron carriers

Unification Links: DIP:DIP-11829N , EcoliWiki:b1873 , ModBase:P52005 , PR:PRO_000024092 , Protein Model Portal:P52005 , RefSeq:NP_416387 , SMR:P52005 , String:511145.b1873 , UniProt:P52005

Relationship Links: InterPro:IN-FAMILY:IPR005126 , InterPro:IN-FAMILY:IPR009154 , InterPro:IN-FAMILY:IPR011031 , Pfam:IN-FAMILY:PF03264 , Prosite:IN-FAMILY:PS51008

Summary:
TorY is a c-type cytochrome that is anchored to the inner membrane; 5 predicted heme binding sites (CXXCH) have been identified. TorY has 36% identity with "TorC" [Gon00].

Essentiality data for torY knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Buc99: Buc J, Santini CL, Giordani R, Czjzek M, Wu LF, Giordano G (1999). "Enzymatic and physiological properties of the tungsten-substituted molybdenum TMAO reductase from Escherichia coli." Mol Microbiol 32(1);159-68. PMID: 10216869

delCampillo96: del Campillo Campbell A, Campbell A (1996). "Alternative gene for biotin sulfoxide reduction in Escherichia coli K-12." J Mol Evol 42(2);85-90. PMID: 8919859

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gon00: Gon S, Patte JC, Mejean V, Iobbi-Nivol C (2000). "The torYZ (yecK bisZ) operon encodes a third respiratory trimethylamine N-oxide reductase in Escherichia coli." J Bacteriol 2000;182(20);5779-86. PMID: 11004177

IobbiNivol96: Iobbi-Nivol C, Pommier J, Simala-Grant J, Mejean V, Giordano G (1996). "High substrate specificity and induction characteristics of trimethylamine-N-oxide reductase of Escherichia coli." Biochim Biophys Acta 1996;1294(1);77-82. PMID: 8639717

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yamamoto86: Yamamoto I, Okubo N, Ishimoto M (1986). "Further characterization of trimethylamine N-oxide reductase from Escherichia coli, a molybdoprotein." J Biochem (Tokyo) 1986;99(6);1773-9. PMID: 3528139


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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