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Escherichia coli K-12 substr. MG1655 Transporter: predicted PTS permease CmtBA

Synonyms: EIICmt, Enzyme IICmt

Subunit composition of predicted PTS permease CmtBA = [CmtB][CmtA]
         predicted PTS permease - CmtB subunit = CmtB (summary available)
         predicted PTS permease - CmtA subunit = CmtA (summary available)

Summary:
CmtBA, the "cryptic mannitol" PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its substrates in a process called group translocation (reviewed in [Postma93]). cmtA and cmtB may encode a mannitol-like PTS permease. The two genes complement a mannitol-negative E. coli mutant when expressed using a heterologous promoter [Sprenger93a]. The cmt genes have a lower G+C content than the mtlA operon [Sprenger93a].

The overall PTS-mediated phosphoryl transfer reaction, requiring the two general energy coupling proteins of the PTS, Enzyme I (PtsI) and HPr (PtsH), as well as the three domains of the Enzyme IICmt complex is:

PEP → Enzyme I-Phis189 → HPr-Phis15 → Enzyme IIA-Phis67 → Enzyme IIB-Pcys377 - (Enzyme IIC) → sugar-P

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis Inferred from experiment [Sprenger93a]

Credits:
Last-Curated ? 17-Mar-2014 by Mackie A , Macquarie University


Enzymatic reaction of: D-mannitol transport (predicted PTS permease CmtBA)

Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.

Kinetic Parameters:

Substrate
Km (μM)
Citations
D-mannitol
5000.0
[Begley96a, BRENDA14]
D-mannitol
11.0
[Jacobson83, BRENDA14]

pH(opt): 6 [BRENDA14, Saier80], 6.5 [BRENDA14, Jacobson83], 7 [BRENDA14, Jacobson79], 7.5 [BRENDA14, Sutrina87], 8.5 [BRENDA14, Jacobson83], 9 [BRENDA14, Jacobson79], 9.5 [BRENDA14, Saier80]


Subunit of predicted PTS permease CmtBA: predicted PTS permease - CmtB subunit

Synonyms: TolM, Enzyme IIACmt, EIIACmt, Enzyme IIICmt

Gene: cmtB Accession Numbers: EG11791 (EcoCyc), b2934, ECK2929

Locations: cytosol

Sequence Length: 147 AAs

Molecular Weight: 16.046 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11a, Sprenger93a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Sprenger93a]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Unification Links: EcoliWiki:b2934 , ModBase:P69824 , PR:PRO_000022306 , Protein Model Portal:P69824 , RefSeq:NP_417409 , SMR:P69824 , String:511145.b2934 , UniProt:P69824

Relationship Links: InterPro:IN-FAMILY:IPR002178 , InterPro:IN-FAMILY:IPR016152 , PDB:Structure:2OQ3 , Pfam:IN-FAMILY:PF00359 , Prosite:IN-FAMILY:PS00372 , Prosite:IN-FAMILY:PS51094

Summary:
cmtB encodes a hydrophilic protein with seqence similarity to the EIIA domain of MltA, the mannitol PTS permease [Sprenger93a]. Expression of cmtB from a lac promoter partially complements the growth defect of an E. coli mtlA C-terminal deletion mutant [Sprenger93a]. A solution structure of CmtB shows high similarity with that of the MltA IIA domain although structural differences around the active site are present. Histidine residue 67 is predicted to be the site of phosphorylation [Yu07a].

Essentiality data for cmtB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of predicted PTS permease CmtBA: predicted PTS permease - CmtA subunit

Synonyms: EIICBCmt, Enzyme IICBCmt

Gene: cmtA Accession Numbers: EG11792 (EcoCyc), b2933, ECK2928

Locations: inner membrane

Sequence Length: 462 AAs

Molecular Weight: 48.971 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11a, GOA01a, Sprenger93a]
GO:0015797 - mannitol transport Inferred by computational analysis [GOA01a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01, GOA01a]
Molecular Function: GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
GO:0022872 - protein-N(PI)-phosphohistidine-mannitol phosphotransferase system transmembrane transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Sprenger93a]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Unification Links: EcoliWiki:b2933 , PR:PRO_000022305 , Protein Model Portal:P69826 , RefSeq:NP_417408 , SMR:P69826 , String:511145.b2933 , Swiss-Model:P69826 , UniProt:P69826

Relationship Links: InterPro:IN-FAMILY:IPR003352 , InterPro:IN-FAMILY:IPR003501 , InterPro:IN-FAMILY:IPR004718 , InterPro:IN-FAMILY:IPR013011 , InterPro:IN-FAMILY:IPR013014 , Pfam:IN-FAMILY:PF02302 , Pfam:IN-FAMILY:PF02378 , Prosite:IN-FAMILY:PS51099 , Prosite:IN-FAMILY:PS51104

Summary:
CmtA has 52% amino acid identity with the EIICB domain of MltA, the mannitol PTS permease. cmtA may encode a protein with 6 transmembrane regions; a conserved cysteine residue at position 377 may be required for phosphoryl transfer to substrate. Expression of cmtA under the control of heterogous promoter led to mannitol transport in mtlA mutants of E. coli K-12 [Sprenger93a].

Essentiality data for cmtA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Begley96a: Begley GS, Warner KA, Arents JC, Postma PW, Jacobson GR (1996). "Isolation and characterization of a mutation that alters the substrate specificity of the Escherichia coli glucose permease." J Bacteriol 178(3);940-2. PMID: 8550539

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jacobson79: Jacobson GR, Lee CA, Saier MH (1979). "Purification of the mannitol-specific enzyme II of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system." J Biol Chem 254(2);249-52. PMID: 368051

Jacobson83: Jacobson GR, Lee CA, Leonard JE, Saier MH (1983). "Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease." J Biol Chem 258(17);10748-56. PMID: 6350293

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Saier80: Saier MH (1980). "Catalytic activities associated with the enzymes II of the bacterial phosphotransferase system." J Supramol Struct 14(3);281-94. PMID: 7012451

Sprenger93a: Sprenger GA (1993). "Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various gram-positive bacteria." Biochim Biophys Acta 1993;1158(1);103-6. PMID: 8353127

Sutrina87: Sutrina SL, Waygood EB, Grenier FC, Saier MH (1987). "HPr/HPr-P phosphoryl exchange reaction catalyzed by the mannitol specific enzyme II of the bacterial phosphotransferase system." J Biol Chem 262(6);2636-41. PMID: 3102473

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yu07a: Yu C, Li Y, Xia B, Jin C (2007). "Solution structure of the cryptic mannitol-specific phosphotransferase enzyme IIA CmtB from Escherichia coli." Biochem Biophys Res Commun 362(4);1001-6. PMID: 17803963


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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