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Escherichia coli K-12 substr. MG1655 Transporter: Zn2+ / Cd2+ / Ni2+ / Cu2+ efflux transporter ZitB



Gene: zitB Accession Numbers: G6393 (EcoCyc), b0752, ECK0741

Synonyms: ybgR, ZitB zinc CDF transporter

Regulation Summary Diagram: ?

Summary:
ZitB (formerly known as YbgR) is a probable Zn2+ transporter. It is a member of the cation diffusion facilitator family of metal ion efflux proteins [Paulsen97]. Metal ion uptake and solid state NMR studies indicate that ZitB maybe able to transport not only zinc but also cadmium, nickel and copper [Rahman08b].

Studies have shown that disruption of zntA and zitB results in a greater degree of Zn2+ sensitivity than disruption of zntA alone [Grass01a]. Furthermore, overexpression of zitB results in a significant increase in Zn2+ resistance and a decrease in Zn2+ accumulation. Thus, ZitB probably functions as a Zn2+ efflux system possibly playing a role in zinc homeostasis at low Zn2+ levels. Transcription is Zn2+ inducible, based on zitB-lacZ gene fusion studies [Grass01a]. zitB is constitutively expressed and plays a role as a first-line defense against excess zinc [Wang12d].

Membrane topology predictions using experimentally determined C terminus locations indicate that ZitB has 6 transmembrane helices and the C-terminus is located in the cytoplasm [Rapp04].

Locations: inner membrane

Map Position: [783,105 <- 784,046] (16.88 centisomes)
Length: 942 bp / 313 aa

Molecular Weight of Polypeptide: 34.678 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002549 , EchoBASE:EB3426 , EcoGene:EG13662 , EcoliWiki:b0752 , OU-Microarray:b0752 , PortEco:zitB , PR:PRO_000024248 , Protein Model Portal:P75757 , RefSeq:NP_415273 , RegulonDB:G6393 , SMR:P75757 , String:511145.b0752 , UniProt:P75757

Relationship Links: InterPro:IN-FAMILY:IPR002524 , InterPro:IN-FAMILY:IPR023500 , InterPro:IN-FAMILY:IPR027469 , Panther:IN-FAMILY:PTHR11562 , Pfam:IN-FAMILY:PF01545

In Paralogous Gene Group: 189 (15 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006829 - zinc ion transport Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA06, Grass01a]
GO:0071577 - zinc ion transmembrane transport Inferred by computational analysis Inferred from experiment [Grass01a, GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11a]
GO:0098655 - cation transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0005385 - zinc ion transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA01a, Grass01a]
GO:0008324 - cation transmembrane transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06, DiazMejia09, Daley05, Rapp04]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for zitB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: Zn2+:H+ antiporter (Zn2+ / Cd2+ / Ni2+ / Cu2+ efflux transporter ZitB)

Alternative Products for Zn2+: Cd2+ [Rahman08b ] , Ni2+ [Rahman08b ] , Cu2+ [Rahman08b ]


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 21 -> 41
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 48 -> 68
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 90 -> 110
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 122 -> 142
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 160 -> 180
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 182 -> 202
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Protein-Segment 240 -> 313
[UniProt09]
UniProt: His-rich; Sequence Annotation Type: compositionally biased region;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Grass01a: Grass G, Fan B, Rosen BP, Franke S, Nies DH, Rensing C (2001). "ZitB (YbgR), a member of the cation diffusion facilitator family, is an additional zinc transporter in Escherichia coli." J Bacteriol 2001;183(15);4664-7. PMID: 11443104

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Paulsen97: Paulsen IT, Saier MH (1997). "A novel family of ubiquitous heavy metal ion transport proteins." J Membr Biol 1997;156(2);99-103. PMID: 9075641

Rahman08b: Rahman M, Patching SG, Ismat F, Henderson PJ, Herbert RB, Baldwin SA, McPherson MJ (2008). "Probing metal ion substrate-binding to the E. coli ZitB exporter in native membranes by solid state NMR." Mol Membr Biol 25(8);683-90. PMID: 19039702

Rapp04: Rapp M, Drew D, Daley DO, Nilsson J, Carvalho T, Melen K, De Gier JW, Von Heijne G (2004). "Experimentally based topology models for E. coli inner membrane proteins." Protein Sci 13(4);937-45. PMID: 15044727

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wang12d: Wang D, Hosteen O, Fierke CA (2012). "ZntR-mediated transcription of zntA responds to nanomolar intracellular free zinc." J Inorg Biochem 111;173-81. PMID: 22459916


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 20, 2014, biocyc13.