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Escherichia coli K-12 substr. MG1655 Transporter: aromatic amino acid:H+ symporter AroP



Gene: aroP Accession Numbers: EG10084 (EcoCyc), b0112, ECK0111

Synonyms: aroR, AroP phenylalanine/tyrosine/tryptophan APC transporter

Regulation Summary Diagram: ?

Summary:
AroP is an aromatic amino acid permease that is a member of the APC Superfamily of transporters [Young99]. AroP is referred to as the general aromatic amino acid permease because it is responsible for the transport of all three aromatic amino acids, phenylalanine, tyrosine, and tryptophan, across the inner membrane [Brown70, Cosgriff97]. Based on the hydrophobicity profile and distribution of charged amino acid residues, AroP was shown to have 12 membrane-spanning regions connected by hydrophilic loops [Cosgriff97]. This topographical model is very similar to that reported for the PheP permease, a phenylalanine-specific transporter, which has 61% amino acid sequence identity with the AroP permease. However, despite their high degree of sequence similarity, the substrate specificities and affinities of the AroP and PheP permeases differ [Cosgriff00]. AroP transports each of the aromatic amino acids with a Km of less than 1 μM [Brown70]. A key residue involved in tryptophan transport is tyrosine at position 103 [Cosgriff00]. Studies with uncouplers of oxidative phosphorylation and with strains deficient in F0F1-ATPase indicate that transport via the AroP system is driven by the proton motive force [Brown70, Cosgriff00].

Expression of aroP is subject to regulation involving the TyrR protein [Whipp77].

Gene Citations: [Honore90, Wang97c, Chye87]

Locations: inner membrane

Map Position: [120,178 <- 121,551] (2.59 centisomes)
Length: 1374 bp / 457 aa

Molecular Weight of Polypeptide: 49.69 kD (from nucleotide sequence), 37.0 kD (experimental) [Chye86 ]

Unification Links: ASAP:ABE-0000384 , CGSC:998 , EchoBASE:EB0082 , EcoGene:EG10084 , EcoliWiki:b0112 , OU-Microarray:b0112 , PortEco:aroP , PR:PRO_000022153 , Protein Model Portal:P15993 , RefSeq:NP_414654 , RegulonDB:EG10084 , SMR:P15993 , String:511145.b0112 , UniProt:P15993

Relationship Links: InterPro:IN-FAMILY:IPR002293 , InterPro:IN-FAMILY:IPR004840 , InterPro:IN-FAMILY:IPR004841 , Panther:IN-FAMILY:PTHR11785 , Pfam:IN-FAMILY:PF00324 , Prosite:IN-FAMILY:PS00218

In Paralogous Gene Group: 37 (10 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0015823 - phenylalanine transport Inferred from experiment [Brown70, Cosgriff97]
GO:0015827 - tryptophan transport Inferred from experiment [Brown70]
GO:0015828 - tyrosine transport Inferred from experiment [Cosgriff97, Brown70]
GO:0071806 - protein transmembrane transport Inferred from experiment [Brown70]
GO:0003333 - amino acid transmembrane transport Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0005302 - L-tyrosine transmembrane transporter activity Inferred from experiment [Brown70, Cosgriff97]
GO:0009977 - proton motive force dependent protein transmembrane transporter activity Inferred from experiment [Brown70]
GO:0015192 - L-phenylalanine transmembrane transporter activity Inferred from experiment [Brown70, Cosgriff97]
GO:0015196 - L-tryptophan transmembrane transporter activity Inferred from experiment [Brown70]
GO:0015171 - amino acid transmembrane transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Cosgriff97]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids phenylalanine
metabolism biosynthesis of building blocks amino acids tyrosine
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for aroP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: L-tryptophan:H+ symporter (aromatic amino acid:H+ symporter AroP)

Synonyms: Transport of L-tryptophan

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-tryptophan
0.4
[Brown70]


Enzymatic reaction of: L-tyrosine:H+ symporter (aromatic amino acid:H+ symporter AroP)

Synonyms: Transport of L-tyrosine

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-tyrosine
0.57
[Brown70]


Enzymatic reaction of: L-phenylalanine:H+ symporter (aromatic amino acid:H+ symporter AroP)

Synonyms: Transport of L-phenylalanine

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-phenylalanine
0.47
[Brown70]


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 21 -> 41
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 28
[Honore90, UniProt10a]
Alternate sequence: A → S; UniProt: (in Ref. 1; CAA35211);
Transmembrane-Region 43 -> 63
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 87 -> 107
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 118 -> 138
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 156 -> 176
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 199
[Cosgriff97, Honore90, UniProt10a]
Alternate sequence: G → A; UniProt: (in Ref. 1; CAA35211 and 2; AAC45299);
Transmembrane-Region 202 -> 222
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 241 -> 261
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 272 -> 292
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 334 -> 354
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 359 -> 379
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 398 -> 399
[Honore90, UniProt10a]
Alternate sequence: PA → L; UniProt: (in Ref. 1; CAA35211);
Transmembrane-Region 401 -> 421
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 424
[Cosgriff97, Honore90, UniProt10a]
Alternate sequence: P → R; UniProt: (in Ref. 1; CAA35211 and 2; AAC45299);
Transmembrane-Region 426 -> 446
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 427
[Honore90, UniProt10a]
Alternate sequence: A → G; UniProt: (in Ref. 1; CAA35211);
Sequence-Conflict 429
[Honore90, UniProt10a]
Alternate sequence: S → W; UniProt: (in Ref. 1; CAA35211);


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
3/2/1998 (pkarp) Merged genes G389/b0112 and EG10084/aroP
10/20/97 Gene b0112 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10084; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brown70: Brown KD (1970). "Formation of aromatic amino acid pools in Escherichia coli K-12." J Bacteriol 1970;104(1);177-88. PMID: 4919744

Chye86: Chye ML, Guest JR, Pittard J (1986). "Cloning of the aroP gene and identification of its product in Escherichia coli K-12." J Bacteriol 1986;167(2);749-53. PMID: 3015892

Chye87: Chye ML, Pittard J (1987). "Transcription control of the aroP gene in Escherichia coli K-12: analysis of operator mutants." J Bacteriol 1987;169(1);386-93. PMID: 3025182

Cosgriff00: Cosgriff AJ, Brasier G, Pi J, Dogovski C, Sarsero JP, Pittard AJ (2000). "A study of AroP-PheP chimeric proteins and identification of a residue involved in tryptophan transport." J Bacteriol 2000;182(8);2207-17. PMID: 10735864

Cosgriff97: Cosgriff AJ, Pittard AJ (1997). "A topological model for the general aromatic amino acid permease, AroP, of Escherichia coli." J Bacteriol 1997;179(10);3317-23. PMID: 9150230

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Honore90: Honore N, Cole ST (1990). "Nucleotide sequence of the aroP gene encoding the general aromatic amino acid transport protein of Escherichia coli K-12: homology with yeast transport proteins." Nucleic Acids Res 18(3);653. PMID: 2408019

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wang97c: Wang P, Yang J, Pittard AJ (1997). "Promoters and transcripts associated with the aroP gene of Escherichia coli." J Bacteriol 1997;179(13);4206-12. PMID: 9209034

Whipp77: Whipp MJ, Pittard AJ (1977). "Regulation of aromatic amino acid transport systems in Escherichia coli K-12." J Bacteriol 132(2);453-61. PMID: 334742

Young99: Young GB, Jack DL, Smith DW, Saier MH (1999). "The amino acid/auxin:proton symport permease family." Biochim Biophys Acta 1999;1415(2);306-22. PMID: 9889387

Other References Related to Gene Regulation

Sarkar08: Sarkar D, Siddiquee KA, Arauzo-Bravo MJ, Oba T, Shimizu K (2008). "Effect of cra gene knockout together with edd and iclR genes knockout on the metabolism in Escherichia coli." Arch Microbiol 190(5);559-71. PMID: 18648770

Shimada11a: Shimada T, Yamamoto K, Ishihama A (2011). "Novel Members of the Cra Regulon Involved in Carbon Metabolism in Escherichia coli." J Bacteriol 193(3);649-59. PMID: 21115656

Wang03a: Wang JG, Fan CS, Wu YQ, Jin RL, Liu DX, Shang L, Jiang PH (2003). "Regulation of aroP expression by tyrR gene in Escherichia coli." Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35(11);993-7. PMID: 14614536

Wang97a: Wang P, Yang J, Lawley B, Pittard AJ (1997). "Repression of the aroP gene of Escherichia coli involves activation of a divergent promoter." J Bacteriol 179(13);4213-8. PMID: 9209035

Wang98b: Wang P, Yang J, Ishihama A, Pittard AJ (1998). "Demonstration that the TyrR protein and RNA polymerase complex formed at the divergent P3 promoter inhibits binding of RNA polymerase to the major promoter, P1, of the aroP gene of Escherichia coli." J Bacteriol 1998;180(20);5466-72. PMID: 9765583

Yang99: Yang J, Wang P, Pittard AJ (1999). "Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli." J Bacteriol 1999;181(20);6411-8. PMID: 10515932


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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