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Escherichia coli K-12 substr. MG1655 Enzyme: argininosuccinate lyase

Gene: argH Accession Numbers: EG11223 (EcoCyc), b3960, ECK3951

Regulation Summary Diagram: ?

Regulation summary diagram for argH

Argininosuccinate lyase catalyzes the final step in the L-arginine biosynthesis pathway.

A crystal structure of argininosuccinate lyase has been solved at 2.44 Å resolution; the enzyme appears to be tetrameric, with a dimer as the asymmetric unit of this crystal form [Bhaumik04].

ArgH expression is repressed by the addition of arginine or ornithine to the medium [Kryzek76].

Gene Citations: [Natter77, Sens77]

Locations: cytosol

Map Position: [4,154,873 -> 4,156,246] (89.55 centisomes, 322°)
Length: 1374 bp / 457 aa

Molecular Weight of Polypeptide: 50.318 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012969 , CGSC:1014 , DIP:DIP-9142N , EchoBASE:EB1205 , EcoGene:EG11223 , EcoliWiki:b3960 , Entrez-gene:948463 , ModBase:P11447 , OU-Microarray:b3960 , PortEco:argH , Pride:P11447 , Protein Model Portal:P11447 , RefSeq:NP_418395 , RegulonDB:EG11223 , SMR:P11447 , String:511145.b3960 , UniProt:P11447

Relationship Links: InterPro:IN-FAMILY:IPR000362 , InterPro:IN-FAMILY:IPR008948 , InterPro:IN-FAMILY:IPR009049 , InterPro:IN-FAMILY:IPR020557 , InterPro:IN-FAMILY:IPR022761 , InterPro:IN-FAMILY:IPR024083 , InterPro:IN-FAMILY:IPR029419 , Panther:IN-FAMILY:PTHR11444 , Panther:IN-FAMILY:PTHR11444:SF3 , PDB:Structure:1TJ7 , Pfam:IN-FAMILY:PF00206 , Pfam:IN-FAMILY:PF14698 , Prints:IN-FAMILY:PR00149 , Prosite:IN-FAMILY:PS00163

In Paralogous Gene Group: 317 (2 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for argH

GO Terms:

Biological Process: GO:0006526 - arginine biosynthetic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11a, GOA06]
GO:0008652 - cellular amino acid biosynthetic process Inferred by computational analysis [UniProtGOA11a]
GO:0042450 - arginine biosynthetic process via ornithine Inferred by computational analysis [GOA01a]
Molecular Function: GO:0004056 - argininosuccinate lyase activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01, GOA01a, Zidwick82]
GO:0003824 - catalytic activity Inferred by computational analysis [GOA01a]
GO:0016829 - lyase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids arginine

Essentiality data for argH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 0.4% glucose No 37 Aerobic 7.2 0.27 No [Patrick07, Comment 3]
M9 medium with 1% glycerol No 37 Aerobic 7.2 0.35 No [Joyce06]
MOPS medium with 0.4% glucose No 37 Aerobic 7.2 0.22 No [Baba06, Comment 2]
No [Feist07, Comment 4]

Last-Curated ? 10-Jul-2006 by Keseler I , SRI International

Enzymatic reaction of: argininosuccinate lyase

Synonyms: arginosuccinase, N-(L-argininosuccinate)arginine-lyase

EC Number:

L-arginino-succinate <=> L-arginine + fumarate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible. [Bhaumik04]

In Pathways: superpathway of arginine and polyamine biosynthesis , L-arginine biosynthesis I (via L-ornithine)

Activators (Unknown Mechanism): Co2+ , Ni2+ [Vogel74]

Inhibitors (Unknown Mechanism): a sulfhydryl reagent [Vogel74] , Zn2+ [Vogel74]

Sequence Features

Protein sequence of argininosuccinate lyase with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 28
[Charlier82, UniProt10]
UniProt: (in Ref. 5; AAB59147);
Sequence-Conflict 40
[Charlier82, UniProt10]
UniProt: (in Ref. 5; AAB59147);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b3960 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11223; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bhaumik04: Bhaumik P, Koski MK, Bergmann U, Wierenga RK (2004). "Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli." Acta Crystallogr D Biol Crystallogr 60(Pt 11);1964-70. PMID: 15502303

Charlier82: Charlier D, Piette J, Glansdorff N (1982). "IS3 can function as a mobile promoter in E. coli." Nucleic Acids Res 10(19);5935-48. PMID: 6292860

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kryzek76: Kryzek RA, Rogers P (1976). "Dual regulation by arginine of the expression of the Escherichia coli argECBH operon." J Bacteriol 126(1);348-64. PMID: 770426

Natter77: Natter W, Sens D, James E (1977). "Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12." J Bacteriol 1977;131(1);214-23. PMID: 326762

Patrick07: Patrick WM, Quandt EM, Swartzlander DB, Matsumura I (2007). "Multicopy suppression underpins metabolic evolvability." Mol Biol Evol 24(12);2716-22. PMID: 17884825

Sens77: Sens D, Natter W, James E (1977). "In vitro transcription of the Escherichia coli K-12 argA, argE, and argCBH operons." J Bacteriol 1977;130(2);642-55. PMID: 400784

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Vogel74: Vogel HJ, Vogel RH (1974). "Enzymes of arginine biosynthesis and their repressive control." Adv Enzymol Relat Areas Mol Biol 1974;40(0);65-90. PMID: 4365537

Zidwick82: Zidwick MJ, Rogers P (1982). "The determination by radiochemical assay of argininosuccinase produced in an Escherichia coli system in vitro." Biochem J 207(3);529-33. PMID: 6762207

Other References Related to Gene Regulation

Caldara06: Caldara M, Charlier D, Cunin R (2006). "The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation." Microbiology 152(Pt 11);3343-54. PMID: 17074904

Charlier92: Charlier D, Roovers M, Van Vliet F, Boyen A, Cunin R, Nakamura Y, Glansdorff N, Pierard A (1992). "Arginine regulon of Escherichia coli K-12. A study of repressor-operator interactions and of in vitro binding affinities versus in vivo repression." J Mol Biol 1992;226(2);367-86. PMID: 1640456

Cunin83: Cunin R, Eckhardt T, Piette J, Boyen A, Pierard A, Glansdorff N (1983). "Molecular basis for modulated regulation of gene expression in the arginine regulon of Escherichia coli K-12." Nucleic Acids Res 1983;11(15);5007-19. PMID: 6348703

Makarova01: Makarova KS, Mironov AA, Gelfand MS (2001). "Conservation of the binding site for the arginine repressor in all bacterial lineages." Genome Biol 2(4);RESEARCH0013. PMID: 11305941

Piette82: Piette J, Cunin R, Boyen A, Charlier D, Crabeel M, Van Vliet F, Glansdorff N, Squires C, Squires CL (1982). "The regulatory region of the divergent argECBH operon in Escherichia coli K-12." Nucleic Acids Res 10(24);8031-48. PMID: 6761650

Weerasinghe06: Weerasinghe JP, Dong T, Schertzberg MR, Kirchhof MG, Sun Y, Schellhorn HE (2006). "Stationary phase expression of the arginine biosynthetic operon argCBH in Escherichia coli." BMC Microbiol 6;14. PMID: 16504055

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sun Aug 30, 2015, biocyc11.