Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
Updated BioCyc iOS App now
available in iTunes store
twitter

Escherichia coli K-12 substr. MG1655 Transporter: leucine ABC transporter


Subunit composition of leucine ABC transporter = [LivK][LivM][LivH][LivG][LivF]
         leucine ABC transporter - periplasmic binding protein = LivK (summary available)
         branched chain amino acid transporter - membrane subunit = LivM
         branched chain amino acid transporter - membrane subunit = LivH
         branched chain amino acid transporter - ATP binding subunit = LivG
         branched chain amino acid ABC transporter - ATP binding subunit = LivF

Summary:
LivFGHMJ and LivFGHMK are two ATP-dependent high-affinity branched-chain amino acid transport system and are members of the ATP Binding Cassette (ABC) Superfamily of transporters [Igarashi99]. The two systems are responsible for the high affinity transport of branched-chain amino acids in E. coli. They have shared membrane and ATP-binding components but have distinctive periplasmic binding proteins. Due to the different periplasmic binding components, the two complexes differ in their binding specificity: LivFGHMK is specific for the transport of leucine, while LivFGHMJ is a transporter for leucine, isoleucine, and valine [Nazos85]. Based on sequence similarity and hydrophobicity analysis, LivJ and LivK are the two periplasmic animo acid-binding proteins, LivH and LivM are the membrane components, and LivG and LivF are the ATP-binding component of the ABC transport complexes [Adams90]. Deletions each of the liv genes resulted in the inability to transport leucine [Adams90]. In addition, a deletion strain that does not express any of the liv genes was unable to carry out high-affinity transport of leucine unless one of the binding protein genes and all of the membrane complex genes were provided on a plasmid [Adams90]. In a separate experiment, liv gene mutants were found to be resistant to a toxic analog of leucine, azaleucine, due to its inability in branched-chain amino acid transport [Nazos85].

Gene-Reaction Schematic

Gene-Reaction Schematic


Enzymatic reaction of: leucine transporter (leucine ABC transporter)

Inferred by computational analysisInferred from experiment

Synonyms: Transport of L-leucine

Transport reaction diagram for leucine transporter


Subunit of leucine ABC transporter: leucine ABC transporter - periplasmic binding protein

Synonyms: HrbC, HrbD, HrbB, LivK

Gene: livK Accession Numbers: EG10540 (EcoCyc), b3458, ECK3442

Locations: periplasmic space

Sequence Length: 369 AAs

Molecular Weight: 39.379 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015807 - L-amino acid transport [Adams90]
Inferred from experimentGO:0015820 - leucine transport [Adams90]
Inferred from experimentGO:0098655 - cation transmembrane transport [Adams90]
Inferred from experimentGO:1902475 - L-alpha-amino acid transmembrane transport [Adams90]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a, GOA01a]
Molecular Function:
Inferred from experimentGO:0015190 - L-leucine transmembrane transporter activity [Adams90]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0030288 - outer membrane-bounded periplasmic space [DiazMejia09, LopezCampistrou05]
Inferred by computational analysisGO:0042597 - periplasmic space [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolismbiosynthesis of building blocksamino acidsleucine
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, periplasmic binding component

Unification Links: EcoliWiki:b3458, ModBase:P04816, PR:PRO_000023098, Pride:P04816, Protein Model Portal:P04816, RefSeq:NP_417915, SMR:P04816, UniProt:P04816

Relationship Links: InterPro:IN-FAMILY:IPR000709, InterPro:IN-FAMILY:IPR028081, InterPro:IN-FAMILY:IPR028082, Panther:IN-FAMILY:PTHR30483:SF6, PDB:Structure:1USG, PDB:Structure:1USI, PDB:Structure:1USK, PDB:Structure:2LBP, Pfam:IN-FAMILY:PF13458, Prints:IN-FAMILY:PR00337

Summary:
The crystal structure of LivK in both the apo- and ligand-bound form has been determined at 1.5 and 1.8 A resolution [Sack89, Magnusson04].

Essentiality data for livK knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid transporter - membrane subunit

Gene: livM Accession Numbers: EG10541 (EcoCyc), b3456, ECK3440

Locations: inner membrane

Sequence Length: 425 AAs

Molecular Weight: 46.269 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015803 - branched-chain amino acid transport [Adams90]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0015658 - branched-chain amino acid transmembrane transporter activity [Adams90]
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell structuremembrane
metabolismbiosynthesis of building blocksamino acidsisoleucine/valine
metabolismbiosynthesis of building blocksamino acidsleucine
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Unification Links: EcoliWiki:b3456, PR:PRO_000023099, Protein Model Portal:P22729, RefSeq:NP_417913, UniProt:P22729

Relationship Links: InterPro:IN-FAMILY:IPR001851, InterPro:IN-FAMILY:IPR021807, Pfam:IN-FAMILY:PF02653, Pfam:IN-FAMILY:PF11862

Essentiality data for livM knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid transporter - membrane subunit

Synonyms: HrbC, HrbD, HrbB

Gene: livH Accession Numbers: EG10538 (EcoCyc), b3457, ECK3441

Locations: inner membrane

Sequence Length: 308 AAs

Molecular Weight: 32.982 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015803 - branched-chain amino acid transport [Adams90]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a]
Molecular Function:
Inferred from experimentGO:0015658 - branched-chain amino acid transmembrane transporter activity [Adams90]
Inferred by computational analysisGO:0005215 - transporter activity [GOA01a]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005886 - plasma membrane [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
Inferred by computational analysisGO:0016020 - membrane [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016021 - integral component of membrane [UniProtGOA11a]

MultiFun Terms: cell structuremembrane
metabolismbiosynthesis of building blocksamino acidsisoleucine/valine
metabolismbiosynthesis of building blocksamino acidsleucine
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily, membrane component

Unification Links: EcoliWiki:b3457, PR:PRO_000023096, Protein Model Portal:P0AEX7, RefSeq:NP_417914, UniProt:P0AEX7

Relationship Links: InterPro:IN-FAMILY:IPR001851, Pfam:IN-FAMILY:PF02653

Essentiality data for livH knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid transporter - ATP binding subunit

Synonyms: HrbC, HrbD, HrbB

Gene: livG Accession Numbers: EG10537 (EcoCyc), b3455, ECK3439

Locations: inner membrane

Sequence Length: 255 AAs

Molecular Weight: 28.427 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentGO:0015803 - branched-chain amino acid transport [Adams90]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a]
Inferred by computational analysisGO:0008152 - metabolic process [GOA01a]
Molecular Function:
Inferred from experimentGO:0015658 - branched-chain amino acid transmembrane transporter activity [Adams90]
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0005524 - ATP binding [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016887 - ATPase activity [GOA01a]
Cellular Component:
Inferred by computational analysisGO:0005886 - plasma membrane [DiazMejia09]

MultiFun Terms: metabolismbiosynthesis of building blocksamino acidsisoleucine/valine
metabolismbiosynthesis of building blocksamino acidsleucine
metabolismcarbon utilizationamino acids
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b3455, ModBase:P0A9S7, PR:PRO_000023095, Pride:P0A9S7, Protein Model Portal:P0A9S7, RefSeq:NP_417912, SMR:P0A9S7, UniProt:P0A9S7

Relationship Links: InterPro:IN-FAMILY:IPR003439, InterPro:IN-FAMILY:IPR003593, InterPro:IN-FAMILY:IPR017871, InterPro:IN-FAMILY:IPR027417, Pfam:IN-FAMILY:PF00005, Pfam:IN-FAMILY:PF12399, Prosite:IN-FAMILY:PS00211, Prosite:IN-FAMILY:PS50893, Smart:IN-FAMILY:SM00382

Essentiality data for livG knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid ABC transporter - ATP binding subunit

Gene: livF Accession Numbers: EG10536 (EcoCyc), b3454, ECK3438

Locations: inner membrane

Sequence Length: 237 AAs

Molecular Weight: 26.31 kD (from nucleotide sequence)


GO Terms:
Biological Process:
Inferred from experimentInferred by computational analysisGO:0015803 - branched-chain amino acid transport [GOA01a, Adams90]
Inferred from experimentGO:0015804 - neutral amino acid transport [Adams90]
Inferred from experimentGO:0015807 - L-amino acid transport [Adams90]
Inferred from experimentGO:0098655 - cation transmembrane transport [Adams90]
Inferred from experimentGO:1902475 - L-alpha-amino acid transmembrane transport [Adams90]
Inferred by computational analysisGO:0006810 - transport [UniProtGOA11a]
Inferred by computational analysisGO:0006865 - amino acid transport [UniProtGOA11a]
Inferred by computational analysisGO:0008152 - metabolic process [GOA01a]
Molecular Function:
Inferred from experimentInferred by computational analysisGO:0015658 - branched-chain amino acid transmembrane transporter activity [GOA01a, Adams90]
Inferred by computational analysisGO:0000166 - nucleotide binding [UniProtGOA11a]
Inferred by computational analysisGO:0005524 - ATP binding [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0016887 - ATPase activity [GOA01a]
Cellular Component:
Inferred by computational analysisGO:0005886 - plasma membrane [DiazMejia09]

MultiFun Terms: metabolismbiosynthesis of building blocksamino acidsisoleucine/valine
metabolismbiosynthesis of building blocksamino acidsleucine
metabolismcarbon utilizationamino acids
transportChannel-type TransportersPyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active TransportersThe ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake PermeasesABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b3454, ModBase:P22731, PR:PRO_000023094, Pride:P22731, Protein Model Portal:P22731, RefSeq:NP_417911, SMR:P22731, Swiss-Model:P22731, UniProt:P22731

Relationship Links: InterPro:IN-FAMILY:IPR003439, InterPro:IN-FAMILY:IPR003593, InterPro:IN-FAMILY:IPR017871, InterPro:IN-FAMILY:IPR027417, InterPro:IN-FAMILY:IPR030660, Pfam:IN-FAMILY:PF00005, Prosite:IN-FAMILY:PS00211, Prosite:IN-FAMILY:PS50893, Smart:IN-FAMILY:SM00382

Essentiality data for livF knockouts:

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enrichedYes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB LennoxYes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerolYes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucoseYes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Adams90: Adams MD, Wagner LM, Graddis TJ, Landick R, Antonucci TK, Gibson AL, Oxender DL (1990). "Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." J Biol Chem 1990;265(20);11436-43. PMID: 2195019

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Igarashi99: Igarashi K, Kashiwagi K (1999). "Polyamine transport in bacteria and yeast." Biochem J 1999;344 Pt 3;633-42. PMID: 10585849

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Magnusson04: Magnusson U, Salopek-Sondi B, Luck LA, Mowbray SL (2004). "X-ray structures of the leucine-binding protein illustrate conformational changes and the basis of ligand specificity." J Biol Chem 279(10);8747-52. PMID: 14672931

Nazos85: Nazos PM, Mayo MM, Su TZ, Anderson JJ, Oxender DL (1985). "Identification of livG, a membrane-associated component of the branched-chain amino acid transport in Escherichia coli." J Bacteriol 1985;163(3);1196-202. PMID: 2993238

Sack89: Sack JS, Trakhanov SD, Tsigannik IH, Quiocho FA (1989). "Structure of the L-leucine-binding protein refined at 2.4 A resolution and comparison with the Leu/Ile/Val-binding protein structure." J Mol Biol 206(1);193-207. PMID: 2649683

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by Pathway Tools version 19.5 (software by SRI International) on Tue May 3, 2016, biocyc13.