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Escherichia coli K-12 substr. MG1655 Transporter: leucine ABC transporter

Subunit composition of leucine ABC transporter = [LivK][LivM][LivH][LivG][LivF]
         leucine ABC transporter - periplasmic binding protein = LivK (summary available)
         branched chain amino acid transporter - membrane subunit = LivM
         branched chain amino acid transporter - membrane subunit = LivH
         branched chain amino acid transporter - ATP binding subunit = LivG
         branched chain amino acid ABC transporter - ATP binding subunit = LivF

Summary:
LivFGHMJ and LivFGHMK are two ATP-dependent high-affinity branched-chain amino acid transport system and are members of the ATP Binding Cassette (ABC) Superfamily of transporters [Igarashi99]. The two systems are responsible for the high affinity transport of branched-chain amino acids in E. coli. They have shared membrane and ATP-binding components but have distinctive periplasmic binding proteins. Due to the different periplasmic binding components, the two complexes differ in their binding specificity: LivFGHMK is specific for the transport of leucine, while LivFGHMJ is a transporter for leucine, isoleucine, and valine [Nazos85]. Based on sequence similarity and hydrophobicity analysis, LivJ and LivK are the two periplasmic animo acid-binding proteins, LivH and LivM are the membrane components, and LivG and LivF are the ATP-binding component of the ABC transport complexes [Adams90]. Deletions each of the liv genes resulted in the inability to transport leucine [Adams90]. In addition, a deletion strain that does not express any of the liv genes was unable to carry out high-affinity transport of leucine unless one of the binding protein genes and all of the membrane complex genes were provided on a plasmid [Adams90]. In a separate experiment, liv gene mutants were found to be resistant to a toxic analog of leucine, azaleucine, due to its inability in branched-chain amino acid transport [Nazos85].

Gene-Reaction Schematic: ?


Enzymatic reaction of: leucine transporter (leucine ABC transporter)

Synonyms: Transport of L-leucine


Subunit of leucine ABC transporter: leucine ABC transporter - periplasmic binding protein

Synonyms: HrbC, HrbD, HrbB, LivK

Gene: livK Accession Numbers: EG10540 (EcoCyc), b3458, ECK3442

Locations: periplasmic space

Sequence Length: 369 AAs

Molecular Weight: 39.379 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015807 - L-amino acid transport Inferred from experiment [Adams90]
GO:0015820 - leucine transport Inferred from experiment [Adams90]
GO:0098655 - cation transmembrane transport Inferred from experiment [Adams90]
GO:1902475 - L-alpha-amino acid transmembrane transport Inferred from experiment [Adams90]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0015190 - L-leucine transmembrane transporter activity Inferred from experiment [Adams90]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [GOA01, DiazMejia09, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids leucine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Unification Links: EcoliWiki:b3458 , ModBase:P04816 , PR:PRO_000023098 , Pride:P04816 , Protein Model Portal:P04816 , RefSeq:NP_417915 , SMR:P04816 , String:511145.b3458 , UniProt:P04816

Relationship Links: InterPro:IN-FAMILY:IPR000709 , InterPro:IN-FAMILY:IPR028081 , InterPro:IN-FAMILY:IPR028082 , Panther:IN-FAMILY:PTHR30483 , PDB:Structure:1USG , PDB:Structure:1USI , PDB:Structure:1USK , PDB:Structure:2LBP , Pfam:IN-FAMILY:PF01094 , Pfam:IN-FAMILY:PF13458 , Prints:IN-FAMILY:PR00337

Summary:
The crystal structure of LivK in both the apo- and ligand-bound form has been determined at 1.5 and 1.8 A resolution [Sack89, Magnusson04].

Essentiality data for livK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid transporter - membrane subunit

Gene: livM Accession Numbers: EG10541 (EcoCyc), b3456, ECK3440

Locations: inner membrane

Sequence Length: 425 AAs

Molecular Weight: 46.269 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015803 - branched-chain amino acid transport Inferred from experiment [Adams90]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0015658 - branched-chain amino acid transmembrane transporter activity Inferred from experiment [Adams90]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids isoleucine/valine
metabolism biosynthesis of building blocks amino acids leucine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: EcoliWiki:b3456 , PR:PRO_000023099 , Protein Model Portal:P22729 , RefSeq:NP_417913 , String:511145.b3456 , UniProt:P22729

Relationship Links: InterPro:IN-FAMILY:IPR001851 , InterPro:IN-FAMILY:IPR021807 , Pfam:IN-FAMILY:PF02653 , Pfam:IN-FAMILY:PF11862

Essentiality data for livM knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid transporter - membrane subunit

Synonyms: HrbC, HrbD, HrbB

Gene: livH Accession Numbers: EG10538 (EcoCyc), b3457, ECK3441

Locations: inner membrane

Sequence Length: 308 AAs

Molecular Weight: 32.982 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015803 - branched-chain amino acid transport Inferred from experiment [Adams90]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0015658 - branched-chain amino acid transmembrane transporter activity Inferred from experiment [Adams90]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids isoleucine/valine
metabolism biosynthesis of building blocks amino acids leucine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: EcoliWiki:b3457 , PR:PRO_000023096 , Protein Model Portal:P0AEX7 , RefSeq:NP_417914 , String:511145.b3457 , UniProt:P0AEX7

Relationship Links: InterPro:IN-FAMILY:IPR001851 , Pfam:IN-FAMILY:PF02653

Essentiality data for livH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid transporter - ATP binding subunit

Synonyms: HrbC, HrbD, HrbB

Gene: livG Accession Numbers: EG10537 (EcoCyc), b3455, ECK3439

Locations: inner membrane

Sequence Length: 255 AAs

Molecular Weight: 28.427 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0015803 - branched-chain amino acid transport Inferred from experiment [Adams90]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0015658 - branched-chain amino acid transmembrane transporter activity Inferred from experiment [Adams90]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids isoleucine/valine
metabolism biosynthesis of building blocks amino acids leucine
metabolism carbon utilization amino acids
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b3455 , ModBase:P0A9S7 , PR:PRO_000023095 , Pride:P0A9S7 , Protein Model Portal:P0A9S7 , RefSeq:NP_417912 , SMR:P0A9S7 , String:511145.b3455 , UniProt:P0A9S7

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Essentiality data for livG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of leucine ABC transporter: branched chain amino acid ABC transporter - ATP binding subunit

Gene: livF Accession Numbers: EG10536 (EcoCyc), b3454, ECK3438

Locations: inner membrane

Sequence Length: 237 AAs

Molecular Weight: 26.31 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006812 - cation transport Inferred from experiment [Adams90]
GO:0015803 - branched-chain amino acid transport Inferred from experiment [Adams90]
GO:0015804 - neutral amino acid transport Inferred from experiment [Adams90]
GO:0015807 - L-amino acid transport Inferred from experiment [Adams90]
GO:0098655 - cation transmembrane transport Inferred from experiment [Adams90]
GO:1902475 - L-alpha-amino acid transmembrane transport Inferred from experiment [Adams90]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0015658 - branched-chain amino acid transmembrane transporter activity Inferred from experiment [Adams90]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids isoleucine/valine
metabolism biosynthesis of building blocks amino acids leucine
metabolism carbon utilization amino acids
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b3454 , ModBase:P22731 , PR:PRO_000023094 , Pride:P22731 , Protein Model Portal:P22731 , RefSeq:NP_417911 , SMR:P22731 , String:511145.b3454 , Swiss-Model:P22731 , UniProt:P22731

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Essentiality data for livF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Adams90: Adams MD, Wagner LM, Graddis TJ, Landick R, Antonucci TK, Gibson AL, Oxender DL (1990). "Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." J Biol Chem 1990;265(20);11436-43. PMID: 2195019

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Igarashi99: Igarashi K, Kashiwagi K (1999). "Polyamine transport in bacteria and yeast." Biochem J 1999;344 Pt 3;633-42. PMID: 10585849

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Magnusson04: Magnusson U, Salopek-Sondi B, Luck LA, Mowbray SL (2004). "X-ray structures of the leucine-binding protein illustrate conformational changes and the basis of ligand specificity." J Biol Chem 279(10);8747-52. PMID: 14672931

Nazos85: Nazos PM, Mayo MM, Su TZ, Anderson JJ, Oxender DL (1985). "Identification of livG, a membrane-associated component of the branched-chain amino acid transport in Escherichia coli." J Bacteriol 1985;163(3);1196-202. PMID: 2993238

Sack89: Sack JS, Trakhanov SD, Tsigannik IH, Quiocho FA (1989). "Structure of the L-leucine-binding protein refined at 2.4 A resolution and comparison with the Leu/Ile/Val-binding protein structure." J Mol Biol 206(1);193-207. PMID: 2649683

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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