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Escherichia coli K-12 substr. MG1655 Transporter: glutamate / aspartate ABC transporter

Subunit composition of glutamate / aspartate ABC transporter = [GltI][GltK][GltJ][GltL]
         glutamate / aspartate ABC transporter - periplasmic binding protein = GltI (summary available)
         glutamate / aspartate ABC transporter - membrane subunit = GltK (summary available)
         glutamate / aspartate ABC transporter - membrane subunit = GltJ (summary available)
         glutamate / aspartate ABC transporter - ATP binding subunit = GltL (summary available)

Summary:
E. coli contains four transporters for the uptake of glutamate (GltIJKL, GltP, GltS, and GadC) and five transporters for the uptake of aspartate (GltIJKL, GltP, DctA, DcuA, and DcuB). GltIJKL accounts for approximately 15% of the total wild-type transport velocity of glutamate, and 25% of the total wild-type transport velocity of aspartate in aerobic growth with succinate and 40 mM NaCl [Schellenberg77].

The GltIJKL glutamate/aspartate transporter is a member of the ATP Binding Cassette (ABC) transporter superfamily [Linton98]. Sequence similarity with known ABC transporter components suggests that GltJK are integral membrane components and GltL is the ABC protein. GltI is the periplasmic-binding component of the GltIJKL glutamate ABC transporter [Linton98]. gltI was shown to be regulated by the FlhDC flagellar transcriptional regulator [Stafford05]. Expression of gltI is greatly reduced when E. coli K-12 strain CS101 is grown in rich medium [Kahane76]. Expression of gltI is also reduced when E. coli W strain D2W is grown with glucose rather than succinate [Willis75].

Citations: [Willis75a, Barash75, Urbanowski00]

Gene-Reaction Schematic: ?


Enzymatic reaction of: transport of L-aspartate (glutamate / aspartate ABC transporter)

EC Number: 3.6.3.21

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-aspartate
0.5
[Schellenberg77]


Enzymatic reaction of: transport of L-glutamate (glutamate / aspartate ABC transporter)

EC Number: 3.6.3.21

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-glutamate
0.5
[Schellenberg77]


Subunit of glutamate / aspartate ABC transporter: glutamate / aspartate ABC transporter - periplasmic binding protein

Synonyms: YzzK, YbeJ, GltBP

Gene: gltI Accession Numbers: G6359 (EcoCyc), b0655, ECK0648

Locations: periplasmic space

Sequence Length: 302 AAs

Molecular Weight: 33.42 kD (from nucleotide sequence)

Molecular Weight: 31.5 kD (experimental) [Urbanowski00]

pI: 9.1 [Barash75]

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Han13, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids glutamate
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Unification Links: DIP:DIP-11361N , EcoliWiki:b0655 , ModBase:P37902 , PR:PRO_000022807 , Pride:P37902 , Protein Model Portal:P37902 , RefSeq:NP_415188 , SMR:P37902 , String:511145.b0655 , UniProt:P37902

Relationship Links: InterPro:IN-FAMILY:IPR001638 , Pfam:IN-FAMILY:PF00497 , Prosite:IN-FAMILY:PS01039 , Smart:IN-FAMILY:SM00062

Summary:
GltI is the periplasmic binding component of the glutamate ABC transporter.

Essentiality data for gltI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of glutamate / aspartate ABC transporter: glutamate / aspartate ABC transporter - membrane subunit

Gene: gltK Accession Numbers: EG12662 (EcoCyc), b0653, ECK0646

Locations: inner membrane

Sequence Length: 224 AAs

Molecular Weight: 24.915 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids aspartate
metabolism biosynthesis of building blocks amino acids glutamate
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: EcoliWiki:b0653 , PR:PRO_000022809 , Protein Model Portal:P0AER5 , RefSeq:NP_415186 , String:511145.b0653 , UniProt:P0AER5

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR010065 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

Summary:
GltK is an integral membrane component of the glutamate ABC transporter.

Essentiality data for gltK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of glutamate / aspartate ABC transporter: glutamate / aspartate ABC transporter - membrane subunit

Gene: gltJ Accession Numbers: EG12661 (EcoCyc), b0654, ECK0647

Locations: inner membrane

Sequence Length: 246 AAs

Molecular Weight: 27.503 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids aspartate
metabolism biosynthesis of building blocks amino acids glutamate
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: DIP:DIP-35965N , EcoliWiki:b0654 , Mint:MINT-1264109 , PR:PRO_000022808 , Pride:P0AER3 , Protein Model Portal:P0AER3 , RefSeq:NP_415187 , String:511145.b0654 , UniProt:P0AER3

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR010065 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

Summary:
GltJ is an integral membrane component of the glutamate ABC transporter.

Essentiality data for gltJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of glutamate / aspartate ABC transporter: glutamate / aspartate ABC transporter - ATP binding subunit

Gene: gltL Accession Numbers: EG12663 (EcoCyc), b0652, ECK0645

Locations: cytosol, inner membrane

Sequence Length: 241 AAs

Molecular Weight: 26.661 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids aspartate
metabolism biosynthesis of building blocks amino acids glutamate
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: DIP:DIP-48246N , EcoliWiki:b0652 , Mint:MINT-1307893 , ModBase:P0AAG3 , PR:PRO_000022810 , Protein Model Portal:P0AAG3 , RefSeq:NP_415185 , SMR:P0AAG3 , String:511145.b0652 , Swiss-Model:P0AAG3 , UniProt:P0AAG3

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Summary:
GltL is the ATP binding component of the glutamate ABC transporter.

Essentiality data for gltL knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Barash75: Barash H, Halpern YS (1975). "Purification and properties of glutamate binding protein from the periplasmic space of Escherichia coli K-12." Biochim Biophys Acta 386(1);168-80. PMID: 236016

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han13: Han MJ, Kim JY, Kim JA (2013). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng. PMID: 24140104

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kahane76: Kahane S, Marcus M, Metzer E, Halpern YS (1976). "Effect of growth conditions on glutamate transport in the wild-type strain and glutamate-utilizing mutants of Escherichia coli." J Bacteriol 125(3);762-9. PMID: 767325

Linton98: Linton KJ, Higgins CF (1998). "The Escherichia coli ATP-binding cassette (ABC) proteins." Mol Microbiol 1998;28(1);5-13. PMID: 9593292

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Schellenberg77: Schellenberg GD, Furlong CE (1977). "Resolution of the multiplicity of the glutamate and aspartate transport systems of Escherichia coli." J Biol Chem 1977;252(24);9055-64. PMID: 336628

Stafford05: Stafford GP, Ogi T, Hughes C (2005). "Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2." Microbiology 151(Pt 6);1779-88. PMID: 15941987

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Urbanowski00: Urbanowski ML, Stauffer LT, Stauffer GV (2000). "The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli." Mol Microbiol 37(4);856-68. PMID: 10972807

Willis75: Willis RC, Furlong CE (1975). "Interactions of a glutamate-aspartate binding protein with the glutamate transport system of Escherichia coli." J Biol Chem 250(7);2581-6. PMID: 1091636

Willis75a: Willis RC, Furlong CE (1975). "Purification and properties of a periplasmic glutamate-aspartate binding protein from Escherichia coli K12 strain W3092." J Biol Chem 250(7);2574-80. PMID: 1091635


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC13B.