Abbrev Name: non-ribosomal peptide synthetase
The biosynthetic system for the production of a non-ribosomal peptide operates nucleic acid-free at the protein level. The specific condensation of amino acids and related carboxyl containing compounds is directed by protein templates, and each biosynthetic step generally requires a protein module. A minimal configuration consists of an adenylation domain for amino acid activation, a thiolation domain for transfer of activated intermediates, and a condensation domain.
Domains and modules are assembled at the gene level into gene clusters, which are translated into multifunctional proteins or multi-enzyme complexes, and post-translationally modified by addition of 4'-phosphopantetheine to the thiolation domain [Welker06].
Since a minimal module requires 3-3.5 kbp of genetic sequence, and since most non-ribosomal peptide synthetases (NRPSs) consist of several such modules, some NRPS genes are the largest known genes [Finking04].
SMILES: CC(C)(COP(=O)([O-])OCC(N[a non-ribosomal peptide synthetase])C(=O)[a non-ribosomal peptide synthetase])C(O)C(=O)NCCC(=O)NCCS
Instance reaction of [an acyl-carrier protein + coenzyme A → adenosine 3',5'-bisphosphate + a holo-[acyl-carrier protein] + H+] (18.104.22.168):
i1: [EntB aryl-carrier protein] + coenzyme A → a holo EntB isochorismatase/aryl-carrier protein + adenosine 3',5'-bisphosphate + H+ (22.214.171.124)
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